AB233519

Anti-ITGB6 antibody

Name: Anti-ITGB6 antibody (ab233519) | Abcam
Brand: Abcam Limited
SKU: AB233519
Price: 525 USD
Availability: InStock
Rating: 5 (3 reviews)

(3 Reviews)

(5 Publications)

Rabbit Polyclonal ITGB6 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Integrin beta-6 aa 100-400.

View Alternative Names

Integrin beta-6, ITGB6

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITGB6 antibody (AB233519)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITGB6 antibody (AB233519)

Formalin-fixed, paraffin-embedded human breast tissue stained for ITGB6 using ab233519 at 10 μg/ml in immunohistochemical analysis. AEC staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITGB6 antibody (AB233519)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for ITGB6 using ab233519 at 10 μg/ml in immunohistochemical analysis. AEC staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITGB6 antibody (AB233519)

Formalin-fixed, paraffin-embedded human prostate tissue stained for ITGB6 using ab233519 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITGB6 antibody (AB233519)

Formalin-fixed, paraffin-embedded human spleen tissue stained for ITGB6 using ab233519 at 10 μg/ml in immunohistochemical analysis. AEC staining.

Supplier Data

Western blot - Anti-ITGB6 antibody (AB233519)

All lanes:

Western blot - Anti-ITGB6 antibody (ab233519) at 2 µg/mL

All lanes:

Recombinant human ITGB6 protein

Predicted band size: 86 kDa

false

Supplier Data

Western blot - Anti-ITGB6 antibody (AB233519)

All lanes:

Western blot - Anti-ITGB6 antibody (ab233519) at 2 µg/mL

All lanes:

Pig skin tissue lysate

Predicted band size: 86 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Pig

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Integrin beta-6 aa 100-400. The exact immunogen used to generate this antibody is proprietary information.

P18564

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein ITGB6 also known as Integrin beta-6 is a part of the integrin family which are transmembrane receptors. ITGB6 has a molecular mass of approximately 88 kDa. It functions mechanically by binding to extracellular matrix proteins such as fibronectin vitronectin and tenascin. ITGB6 pairs exclusively with the integrin alpha-V subunit to form the integrin alpha-V beta-6 (αvβ6) heterodimer. Its expression mainly occurs in epithelial cells particularly in the skin lungs and gastrointestinal tract.

Biological function summary

ITGB6 plays important roles in cell adhesion migration and signaling. It is part of the αv integrin complex which is significant in the regulation of transforming growth factor-beta (TGF-β) activation. This activation influences epithelial-mesenchymal transition (EMT) tissue repair and fibrosis. ITGB6 impacts processes such as inflammation and wound healing due to its expression in epithelial tissues thereby contributing to maintaining tissue integrity and function.

Pathways

ITGB6 interacts in the TGF-β signaling and epithelial cell migration pathways. TGF-β activation via ITGB6 plays a critical role in controlling immune responses and cellular proliferation. ITGB6 is interconnected with other integrin family members such as ITGAV (Integrin alpha-V) which collaborates in cellular mechanisms involving cell adhesion and signaling processes. These interactions contribute to cellular responses to extracellular signals that influence pathophysiological processes.

ITGB6 has associations with fibrosis and cancer. In fibrotic diseases ITGB6 facilitates excess activation of TGF-β leading to aberrant tissue remodeling in organs such as the lungs and liver. Regarding cancer ITGB6 influences tumor progression and metastasis particularly in epithelial-derived cancers due to its role in EMT and tissue invasion. ITGB6's interactions with other integrins and ECM proteins contribute to tumorigenesis and fibrotic tissue changes making it a therapeutic target in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin alpha-V : beta-6 (ITGAV : ITGB6) is a receptor for fibronectin and cytotactin (PubMed : 17158881, PubMed : 17545607). It recognizes the sequence R-G-D in its ligands (PubMed : 17158881, PubMed : 17545607). Internalization of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion (PubMed : 17158881, PubMed : 17545607). ITGAV : ITGB6 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed : 17158881). Integrin alpha-V : beta-6 (ITGAV : ITGB6) mediates R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP), thereby playing a key role in TGF-beta-1 activation (PubMed : 15184403, PubMed : 22278742, PubMed : 28117447).. (Microbial infection) Integrin ITGAV : ITGB6 acts as a receptor for Coxsackievirus A9 and Coxsackievirus B1.. (Microbial infection) Integrin ITGAV : ITGB6 acts as a receptor for Herpes simplex virus-1/HHV-1 (PubMed : 24367260).

See full target information Integrin beta-6

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Annals of translational medicine 9:803 PubMed34268416

2021

Silencing of ITGB6 inhibits the progression of cervical carcinoma via regulating JAK/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxia Zheng,Yanan Zhu,Xiaoping Wang,Yanmei Hou,Yingji Fang

Archives of medical science : AMS 20:267-279 PubMed38414469

2021

may promote proliferation and invasion in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Zhong,Zhi-Xi Li,Ling-Jing Yang,Gang Wu,Bo Xiang,Yu-Lan Wang,Qing Zhou

American journal of physiology. Renal physiology 320:F87-F96 PubMed33283645

2020

Renoprotective effect of deletion in murine aristolochic acid nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Wenguang Feng,Wei-Zhong Ying,Xingsheng Li,Lisa M Curtis,Paul W Sanders

Bone research 8:35 PubMed33083095

2020

Toward diagnostic relevance of the αβ, αβ, and αβ integrins in OA: expression within human cartilage and spinal osteophytes.

Applications

Unspecified application

Species

Unspecified reactive species

Edith Charlier,Céline Deroyer,Sophie Neuville,Zelda Plener,Olivier Malaise,Federica Ciregia,Philippe Gillet,Gilles Reuter,Mallory Salvé,Nadia Withofs,Roland Hustinx,Dominique de Seny,Michel G Malaise

Expert review of proteomics 16:363-373 PubMed30779878

2019

Prediction of lymph node metastasis in oral squamous cell carcinoma based on protein profile.

Applications

Unspecified application

Species

Unspecified reactive species

Binbin Yu,Wei Cao,Chenping Zhang,Ronghui Xia,Jinlin Liu,Ming Yan,Wantao Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ITGB6 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITGB6 antibody (AB233519)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITGB6 antibody (AB233519)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITGB6 antibody (AB233519)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITGB6 antibody (AB233519)

Western blot - Anti-ITGB6 antibody (AB233519)

Western blot - Anti-ITGB6 antibody (AB233519)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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