AB233032

Anti-ITIH1/SHAP antibody

Name: Anti-ITIH1/SHAP antibody (ab233032) | Abcam
Brand: Abcam Limited
SKU: AB233032
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal ITIH1/SHAP antibody. Suitable for WB, IHC-P and reacts with Recombinant fragment - Human, Pig, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ITIH1 aa 500-850.

View Alternative Names

IGHEP1, ITIH1, Inter-alpha-trypsin inhibitor heavy chain H1, ITI heavy chain H1, ITI-HC1, Inter-alpha-inhibitor heavy chain 1, Inter-alpha-trypsin inhibitor complex component III, Serum-derived hyaluronan-associated protein, SHAP

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH1/SHAP antibody (AB233032)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH1/SHAP antibody (AB233032)

Formalin-fixed, paraffin-embedded human liver tissue stained for ITIH1/SHAP using ab233032 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH1/SHAP antibody (AB233032)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for ITIH1/SHAP using ab233032 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH1/SHAP antibody (AB233032)

Formalin-fixed, paraffin-embedded human lung cancer tissue stained for ITIH1/SHAP using ab233032 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH1/SHAP antibody (AB233032)

Formalin-fixed, paraffin-embedded human stomach cancer tissue stained for ITIH1/SHAP using ab233032 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH1/SHAP antibody (AB233032)

Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for ITIH1/SHAP using ab233032 at 10 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH1/SHAP antibody (AB233032)

Formalin-fixed, paraffin-embedded human prostate tissue stained for ITIH1/SHAP using ab233032 at 10 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-ITIH1/SHAP antibody (AB233032)

All lanes:

Western blot - Anti-ITIH1/SHAP antibody (ab233032) at 3 µg/mL

All lanes:

Recombinant human ITIH1/SHAP protein

Secondary

All lanes:

HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution

Predicted band size: 101 kDa

false

Supplier Data

Western blot - Anti-ITIH1/SHAP antibody (AB233032)

All lanes:

Western blot - Anti-ITIH1/SHAP antibody (ab233032) at 3 µg/mL

Lane 1:

Human serum

Lane 2:

Pig heart lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution

Predicted band size: 101 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Pig

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ITIH1 aa 500-850. The exact immunogen used to generate this antibody is proprietary information.

P19827

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target ITIH1 also known as Inter-alpha-trypsin inhibitor heavy chain H1 is a component of the inter-alpha-inhibitor family. ITIH1 has an approximate mass of 100 kDa. This protein is mainly expressed in the liver and secreted into the blood participating in the extracellular matrix. ITIH1 works by stabilizing urinary trypsin inhibitors and playing a role in maintaining structural integrity in tissues especially during inflammation and tissue repair.

Biological function summary

ITIH1 interacts with other macromolecules to form complexes that modulate the extracellular environment. It is part of a complex that involves proteins like bikunin which is important in the formation of the inter-alpha-inhibitor complex. This complex plays important roles in modulating inflammation and is implicated in various physiological processes like immunological responses.

Pathways

ITIH1 is integrated into the acute-phase response pathway which responds to inflammation. It cooperates with proteins such as ITIH2 another member of the inter-alpha-trypsin inhibitor family to mediate immune and inflammatory processes. Furthermore ITIH1 participates in the protective mechanisms against pathogenic invasion through the complement pathway.

ITIH1 has associations with conditions such as sepsis and liver cirrhosis. In sepsis ITIH1 levels may be altered influencing the inflammatory response and affecting patient outcomes. The interaction between ITIH1 and proteins involved in these diseases such as tumor necrosis factor-alpha (TNF-alpha) suggests its role as a potential biomarker or therapeutic target in these pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.. Contains a potential peptide which could stimulate a broad spectrum of phagocytotic cells.

See full target information ITIH1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:1213-1227 PubMed40259083

2025

Multi-omic and spatial analysis of mouse kidneys highlights sex-specific differences in gene regulation across the lifespan.

Applications

Unspecified application

Species

Unspecified reactive species

Siqi Chen,Ruiyang Liu,Chia-Kuei Mo,Michael C Wendl,Andrew Houston,Preet Lal,Yanyan Zhao,Wagma Caravan,Andrew T Shinkle,Atieh Abedin-Do,Nataly Naser Al Deen,Kazuhito Sato,Xiang Li,André Luiz N Targino da Costa,Yize Li,Alla Karpova,John M Herndon,Maxim N Artyomov,Joshua B Rubin,Sanjay Jain,Xue Li,Sheila A Stewart,Li Ding,Feng Chen

Experimental and therapeutic medicine 28:368 PubMed39091412

2024

ITIH1 suppresses carcinogenesis in renal cell carcinoma through regulation of the NF‑κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Gao,Gang Yu,Yan Yan,Weifeng Hu,Dayong Hu,Weibing Wang,Guoxian Yang,Jing Wei,Shiquan Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ITIH1/SHAP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH1/SHAP antibody (AB233032)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH1/SHAP antibody (AB233032)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH1/SHAP antibody (AB233032)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH1/SHAP antibody (AB233032)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH1/SHAP antibody (AB233032)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH1/SHAP antibody (AB233032)

Western blot - Anti-ITIH1/SHAP antibody (AB233032)

Western blot - Anti-ITIH1/SHAP antibody (AB233032)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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