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AB70048

Anti-ITIH1/SHAP antibody

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(6 Publications)

Mouse Polyclonal ITIH1/SHAP antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ITIH1.

View Alternative Names

IGHEP1, ITIH1, Inter-alpha-trypsin inhibitor heavy chain H1, ITI heavy chain H1, ITI-HC1, Inter-alpha-inhibitor heavy chain 1, Inter-alpha-trypsin inhibitor complex component III, Serum-derived hyaluronan-associated protein, SHAP

1 Images
Western blot - Anti-ITIH1/SHAP antibody (AB70048)
  • WB

Unknown

Western blot - Anti-ITIH1/SHAP antibody (AB70048)

All lanes:

Western blot - Anti-ITIH1/SHAP antibody (ab70048) at 1/500 dilution

Lane 1:

ITIH1/SHAP transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 101 kDa

Observed band size: 102 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ITIH1.

P19827

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Alternative Products

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Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] (AB204513)

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH1/SHAP antibody (AB198809)

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  • 0
Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH1/SHAP antibody (AB233032)

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  • 0

Complementary Products

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Primary Antibodies

AB183322

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] (AB183322)

  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target ITIH1 also known as Inter-alpha-trypsin inhibitor heavy chain H1 is a component of the inter-alpha-inhibitor family. ITIH1 has an approximate mass of 100 kDa. This protein is mainly expressed in the liver and secreted into the blood participating in the extracellular matrix. ITIH1 works by stabilizing urinary trypsin inhibitors and playing a role in maintaining structural integrity in tissues especially during inflammation and tissue repair.
Biological function summary

ITIH1 interacts with other macromolecules to form complexes that modulate the extracellular environment. It is part of a complex that involves proteins like bikunin which is important in the formation of the inter-alpha-inhibitor complex. This complex plays important roles in modulating inflammation and is implicated in various physiological processes like immunological responses.

Pathways

ITIH1 is integrated into the acute-phase response pathway which responds to inflammation. It cooperates with proteins such as ITIH2 another member of the inter-alpha-trypsin inhibitor family to mediate immune and inflammatory processes. Furthermore ITIH1 participates in the protective mechanisms against pathogenic invasion through the complement pathway.

ITIH1 has associations with conditions such as sepsis and liver cirrhosis. In sepsis ITIH1 levels may be altered influencing the inflammatory response and affecting patient outcomes. The interaction between ITIH1 and proteins involved in these diseases such as tumor necrosis factor-alpha (TNF-alpha) suggests its role as a potential biomarker or therapeutic target in these pathologies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.. Contains a potential peptide which could stimulate a broad spectrum of phagocytotic cells.
See full target information ITIH1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed39671234

2024

Human birth tissue products as a non-opioid medicine to inhibit post-surgical pain.

Applications

Unspecified application

Species

Unspecified reactive species

Chi Zhang,Qian Huang,Neil C Ford,Nathachit Limjunyawong,Qing Lin,Fei Yang,Xiang Cui,Ankit Uniyal,Jing Liu,Megha Mahabole,Hua He,Xuewei Wang,Irina Duff,Yiru Wang,Jieru Wan,Guangwu Zhu,Srinivasa N Raja,Hongpeng Jia,Dazhi Yang,Xinzhong Dong,Xu Cao,Scheffer C Tseng,Shaoqiu He,Yun Guan

Stem cells (Dayton, Ohio) 39:280-295 PubMed33373496

2021

HC-HA/PTX3 from amniotic membrane reverts senescent limbal niche cells to Pax6+ neural crest progenitors to support limbal epithelial progenitors.

Applications

Unspecified application

Species

Unspecified reactive species

Szu-Yu Chen,Yingting Zhu,Yuan Zhang,David Hsu,Scheffer C G Tseng

Scientific reports 7:43736 PubMed28252047

2017

Inhibition of Proliferation and Epithelial Mesenchymal Transition in Retinal Pigment Epithelial Cells by Heavy Chain-Hyaluronan/Pentraxin 3.

Applications

Unspecified application

Species

Unspecified reactive species

Hua He,Ajay E Kuriyan,Chen-Wei Su,Megha Mahabole,Yuan Zhang,Ying-Ting Zhu,Harry W Flynn,Jean-Marie Parel,Scheffer C G Tseng

Scientific reports 7:42195 PubMed28165063

2017

Heavy Chain-Hyaluronan/Pentraxin 3 from Amniotic Membrane Suppresses Inflammation and Scarring in Murine Lacrimal Gland and Conjunctiva of Chronic Graft-versus-Host Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yoko Ogawa,Hua He,Shin Mukai,Toshihiro Imada,Shigeru Nakamura,Chen-Wei Su,Megha Mahabole,Scheffer C G Tseng,Kazuo Tsubota

The Journal of biological chemistry 289:13531-42 PubMed24652286

2014

Constitutive expression of pentraxin 3 (PTX3) protein by human amniotic membrane cells leads to formation of the heavy chain (HC)-hyaluronan (HA)-PTX3 complex.

Applications

Unspecified application

Species

Unspecified reactive species

Suzhen Zhang,Ying-Ting Zhu,Szu-Yu Chen,Hua He,Scheffer C G Tseng

The Journal of biological chemistry 288:25792-803 PubMed23878196

2013

Immobilized heavy chain-hyaluronic acid polarizes lipopolysaccharide-activated macrophages toward M2 phenotype.

Applications

WB

Species

Human

Hua He,Suzhen Zhang,Sean Tighe,Ji Son,Scheffer C G Tseng
View all publications
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Product promise

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