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Rabbit Recombinant Monoclonal ITIH1/SHAP antibody. Carrier free. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] - BSA and Azide free (AB251413), expandable thumbnail
  • Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] - BSA and Azide free (AB251413), expandable thumbnail
  • Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] - BSA and Azide free (AB251413), expandable thumbnail
  • Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] - BSA and Azide free (AB251413), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Target data

Function

May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes. Contains a potential peptide which could stimulate a broad spectrum of phagocytotic cells.

Alternative names

IGHEP1, ITIH1, Inter-alpha-trypsin inhibitor heavy chain H1, ITI heavy chain H1, ITI-HC1, Inter-alpha-inhibitor heavy chain 1, Inter-alpha-trypsin inhibitor complex component III, Serum-derived hyaluronan-associated protein, SHAP

Recommended products

Rabbit Recombinant Monoclonal ITIH1/SHAP antibody. Carrier free. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR17138-29
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251413 is the carrier-free version of Anti-ITIH1/SHAP antibody [EPR17138-29] ab204513.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The target ITIH1 also known as Inter-alpha-trypsin inhibitor heavy chain H1 is a component of the inter-alpha-inhibitor family. ITIH1 has an approximate mass of 100 kDa. This protein is mainly expressed in the liver and secreted into the blood participating in the extracellular matrix. ITIH1 works by stabilizing urinary trypsin inhibitors and playing a role in maintaining structural integrity in tissues especially during inflammation and tissue repair.

Biological function summary

ITIH1 interacts with other macromolecules to form complexes that modulate the extracellular environment. It is part of a complex that involves proteins like bikunin which is important in the formation of the inter-alpha-inhibitor complex. This complex plays important roles in modulating inflammation and is implicated in various physiological processes like immunological responses.

Pathways

ITIH1 is integrated into the acute-phase response pathway which responds to inflammation. It cooperates with proteins such as ITIH2 another member of the inter-alpha-trypsin inhibitor family to mediate immune and inflammatory processes. Furthermore ITIH1 participates in the protective mechanisms against pathogenic invasion through the complement pathway.

Associated diseases and disorders

ITIH1 has associations with conditions such as sepsis and liver cirrhosis. In sepsis ITIH1 levels may be altered influencing the inflammatory response and affecting patient outcomes. The interaction between ITIH1 and proteins involved in these diseases such as tumor necrosis factor-alpha (TNF-alpha) suggests its role as a potential biomarker or therapeutic target in these pathologies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] - BSA and Azide free (ab251413), expandable thumbnail

    Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] - BSA and Azide free (ab251413)

    This data was developed using Anti-ITIH1/SHAP antibody [EPR17138-29] ab204513, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] (Anti-ITIH1/SHAP antibody [EPR17138-29] ab204513) at 1/1000 dilution

    Lane 1: Human fetal heart tissue lysate at 10 µg

    Lane 2: Human fetal kidney tissue lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 101 kDa

    Observed band size: 125 kDa, 250 kDa, 80 kDa

    Exposure time: 1min

  • Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] - BSA and Azide free (ab251413), expandable thumbnail

    Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] - BSA and Azide free (ab251413)

    This data was developed using Anti-ITIH1/SHAP antibody [EPR17138-29] ab204513, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] (Anti-ITIH1/SHAP antibody [EPR17138-29] ab204513) at 1/10000 dilution

    All lanes: Human brain lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 101 kDa

    Observed band size: 125 kDa, 250 kDa, 80 kDa

    Exposure time: 3min

  • Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] - BSA and Azide free (ab251413), expandable thumbnail

    Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] - BSA and Azide free (ab251413)

    This data was developed using Anti-ITIH1/SHAP antibody [EPR17138-29] ab204513, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] (Anti-ITIH1/SHAP antibody [EPR17138-29] ab204513) at 1/20000 dilution

    All lanes: Human plasma lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 101 kDa

    Observed band size: 125 kDa, 250 kDa, 80 kDa

    Exposure time: 1s

  • Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] - BSA and Azide free (ab251413), expandable thumbnail

    Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] - BSA and Azide free (ab251413)

    This data was developed using Anti-ITIH1/SHAP antibody [EPR17138-29] ab204513, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] (Anti-ITIH1/SHAP antibody [EPR17138-29] ab204513) at 1/50000 dilution

    All lanes: Human serum lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution

    Predicted band size: 101 kDa

    Observed band size: 125 kDa, 250 kDa, 80 kDa

    Exposure time: 2s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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