AB251413

Anti-ITIH1/SHAP antibody [EPR17138-29] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal ITIH1/SHAP antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

IGHEP1, ITIH1, Inter-alpha-trypsin inhibitor heavy chain H1, ITI heavy chain H1, ITI-HC1, Inter-alpha-inhibitor heavy chain 1, Inter-alpha-trypsin inhibitor complex component III, Serum-derived hyaluronan-associated protein, SHAP

4 Images

Supplier Data

Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] - BSA and Azide free (AB251413)

This data was developed using ab204513, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] (<a href='/en-us/products/primary-antibodies/itih1-shap-antibody-epr17138-29-ab204513'>ab204513</a>) at 1/50000 dilution

All lanes:

Human serum lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 101 kDa

Observed band size: 125 kDa,250 kDa,80 kDa

false

Exposure time: 2s

Supplier Data

Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] - BSA and Azide free (AB251413)

This data was developed using ab204513, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] (<a href='/en-us/products/primary-antibodies/itih1-shap-antibody-epr17138-29-ab204513'>ab204513</a>) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 101 kDa

Observed band size: 125 kDa,250 kDa,80 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] - BSA and Azide free (AB251413)

This data was developed using ab204513, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] (<a href='/en-us/products/primary-antibodies/itih1-shap-antibody-epr17138-29-ab204513'>ab204513</a>) at 1/20000 dilution

All lanes:

Human plasma lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 101 kDa

Observed band size: 125 kDa,250 kDa,80 kDa

false

Exposure time: 1s

Supplier Data

Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] - BSA and Azide free (AB251413)

This data was developed using ab204513, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] (<a href='/en-us/products/primary-antibodies/itih1-shap-antibody-epr17138-29-ab204513'>ab204513</a>) at 1/10000 dilution

All lanes:

Human brain lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 101 kDa

Observed band size: 125 kDa,250 kDa,80 kDa

false

Exposure time: 3min

Unconjugated

Anti-ITIH1/SHAP antibody [EPR17138-29]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17138-29

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

ab251413 is the carrier-free version of ab204513.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target ITIH1 also known as Inter-alpha-trypsin inhibitor heavy chain H1 is a component of the inter-alpha-inhibitor family. ITIH1 has an approximate mass of 100 kDa. This protein is mainly expressed in the liver and secreted into the blood participating in the extracellular matrix. ITIH1 works by stabilizing urinary trypsin inhibitors and playing a role in maintaining structural integrity in tissues especially during inflammation and tissue repair.

Biological function summary

ITIH1 interacts with other macromolecules to form complexes that modulate the extracellular environment. It is part of a complex that involves proteins like bikunin which is important in the formation of the inter-alpha-inhibitor complex. This complex plays important roles in modulating inflammation and is implicated in various physiological processes like immunological responses.

Pathways

ITIH1 is integrated into the acute-phase response pathway which responds to inflammation. It cooperates with proteins such as ITIH2 another member of the inter-alpha-trypsin inhibitor family to mediate immune and inflammatory processes. Furthermore ITIH1 participates in the protective mechanisms against pathogenic invasion through the complement pathway.

ITIH1 has associations with conditions such as sepsis and liver cirrhosis. In sepsis ITIH1 levels may be altered influencing the inflammatory response and affecting patient outcomes. The interaction between ITIH1 and proteins involved in these diseases such as tumor necrosis factor-alpha (TNF-alpha) suggests its role as a potential biomarker or therapeutic target in these pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.. Contains a potential peptide which could stimulate a broad spectrum of phagocytotic cells.

See full target information ITIH1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:3596 PubMed34155205

2021

ERRγ enhances cardiac maturation with T-tubule formation in human iPSC-derived cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Kenji Miki,Kohei Deguchi,Misato Nakanishi-Koakutsu,Antonio Lucena-Cacace,Shigeru Kondo,Yuya Fujiwara,Takeshi Hatani,Masako Sasaki,Yuki Naka,Chikako Okubo,Megumi Narita,Ikue Takei,Stephanie C Napier,Tsukasa Sugo,Sachiko Imaichi,Taku Monjo,Tatsuya Ando,Norihisa Tamura,Kenichi Imahashi,Tomoyuki Nishimoto,Yoshinori Yoshida

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ITIH1/SHAP antibody [EPR17138-29] - BSA and Azide free

Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] - BSA and Azide free (AB251413)

Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] - BSA and Azide free (AB251413)

Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] - BSA and Azide free (AB251413)

Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] - BSA and Azide free (AB251413)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise