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AB254773

Anti-ITIH2/SHAP antibody

5

(1 Review)

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(1 Publication)

Rabbit Polyclonal ITIH2/SHAP antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ITIH2 aa 150-300.

View Alternative Names

IGHEP2, ITIH2, Inter-alpha-trypsin inhibitor heavy chain H2, ITI heavy chain H2, ITI-HC2, Inter-alpha-inhibitor heavy chain 2, Inter-alpha-trypsin inhibitor complex component II, Serum-derived hyaluronan-associated protein, SHAP

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH2/SHAP antibody (AB254773)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH2/SHAP antibody (AB254773)

Paraffin-embedded human tonsil tissue stained for ITIH2/SHAP using ab254773 at 1/500 dilution in immunohistochemical analysis. No positivity observed.

Immunocytochemistry/ Immunofluorescence - Anti-ITIH2/SHAP antibody (AB254773)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ITIH2/SHAP antibody (AB254773)

PFA fixed, Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling ITIH2/SHAP using ab254773 at 4μg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH2/SHAP antibody (AB254773)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH2/SHAP antibody (AB254773)

Paraffin-embedded human Fallopian tube tissue stained for ITIH2/SHAP using ab254773 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH2/SHAP antibody (AB254773)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH2/SHAP antibody (AB254773)

Paraffin-embedded human endometrium tissue stained for ITIH2/SHAP using ab254773 at 1/500 dilution in immunohistochemical analysis. No positivity observed.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH2/SHAP antibody (AB254773)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH2/SHAP antibody (AB254773)

Paraffin-embedded human duodenum tissue stained for ITIH2/SHAP using ab254773 at 1/500 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ITIH2 aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

P19823

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ITIH2 also known as Inter-alpha-trypsin inhibitor heavy chain H2 is a protein involved in matrix stabilization during inflammation and tissue repair. With a mass of approximately 125 kDa this protein is primarily expressed in the liver and secreted into the bloodstream. It forms part of the inter-alpha-trypsin inhibitor family where it binds to small glycosaminoglycans such as hyaluronan playing a role in the structural integrity of extracellular matrices.
Biological function summary

ITIH2 functions as a stabilizer in extracellular matrices especially during inflammation and tissue repair processes. It is part of a complex known as the serum-derived hyaluronan-associated protein (SHAP) which contributes to the VCAN (versican) network of the extracellular matrix. This complex participates in organizing matrix assembly and modulating cellular responses during tissue injury.

Pathways

In inflammatory and repair mechanisms ITIH2 plays an important role. It engages in pathways involving extracellular matrix degradation and repair as well as inflammatory response signaling. ITIH2 interacts with proteins such as hyaluronan synthase to influence matrix dynamics. This interaction highlights its participation in significant pathways like those regulating tissue turnover and inflammatory responses.

ITIH2 has been linked to inflammatory conditions and liver disorders. In the context of liver diseases altered levels of ITIH2 may reflect liver pathology such as cirrhosis or hepatitis. Its connection to hyaluronan suggests a relationship with fibrosis where matrix remodeling occurs. Moreover in conditions like rheumatoid arthritis it may interact with C-reactive protein further implying its role in disease processes related to inflammation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.
See full target information ITIH2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinics (Sao Paulo, Brazil) 78:100129 PubMed36473368

2022

Mesenchymal stem cells-derived exosomal miR-653-5p suppresses laryngeal papilloma progression by inhibiting BZW2.

Applications

Unspecified application

Species

Unspecified reactive species

Binya Hu,Min Huang,Lihua Tao,Yun Li,Yuting Kuang,Guangliang Liu,Sijun Zhao
View all publications
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Product promise

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