AB240221

Anti-ITIH4 antibody [EPR11233(2)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal ITIH4 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

IHRP, ITIHL1, PK120, PRO1851, ITIH4, Inter-alpha-trypsin inhibitor heavy chain H4, ITI heavy chain H4, ITI-HC4, Inter-alpha-inhibitor heavy chain 4, Inter-alpha-trypsin inhibitor family heavy chain-related protein, Plasma kallikrein sensitive glycoprotein 120, Gp120, PK-120

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] - BSA and Azide free (AB240221)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] - BSA and Azide free (AB240221)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ITIH4 with ab180139 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180139).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] - BSA and Azide free (AB240221)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling ITIH4 with ab180139 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180139).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-ITIH4 antibody [EPR11233(2)] - BSA and Azide free (AB240221)

Western blot analysis of ITIH4 in immunoprecipitation pellets from Human plasma lysate. ab180139 used at 1/60 dilution for immunoprecipitation. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000. Blocking buffer and concentration : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180139).

All lanes:

Immunoprecipitation - Anti-ITIH4 antibody [EPR11233(2)] (<a href='/en-us/products/primary-antibodies/itih4-antibody-epr112332-ab180139'>ab180139</a>)

Predicted band size: 103 kDa

Observed band size: 120 kDa

false

Unconjugated

Anti-ITIH4 antibody [EPR11233(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11233(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab240221 is the carrier-free version of ab180139.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ITIH4 also known as inter-alpha-trypsin inhibitor heavy chain H4 is a serum protein with a molecular mass of approximately 120 kDa. It belongs to the inter-alpha-trypsin inhibitor (ITI) family. This protein is predominantly expressed in the liver and circulates in the blood. ITIH4 operates mechanically by binding to hyaluronic acid giving it a protective capability in the stabilization of the extracellular matrix.

Biological function summary

ITIH4 contributes to the inflammatory response and tissue repair processes. It forms part of a protease inhibitor complex known as ITI comprised of heavy and light chains that interact together. This complex acts in protecting tissues from damage by inhibiting serine proteases. Additionally ITIH4 has adaptive roles in modulating the inflammatory response facilitating tissue restoration and engaging in immune cell regulation.

Pathways

ITIH4 associates with inflammation and extracellular matrix organization pathways. Inflammation-related pathways involve interactions with several cytokines and matrix metalloproteinases contributing to the regulation of inflammatory responses. Within these pathways ITIH4 exhibits connection with proteins such as interleukin 6 (IL-6) playing roles in inflammation and tissue communication.

ITIH4 has been implicated in liver diseases and certain cancers. Its altered expression and functionality have associations with liver fibrosis where it may influence the extracellular matrix dynamics and hepatocellular carcinoma potentially impacting tumor microenvironments. ITIH4's connections to liver disease also relate it to alpha-1 antitrypsin which plays a part in liver protection and disease modulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Type II acute-phase protein (APP) involved in inflammatory responses to trauma. May also play a role in liver development or regeneration.

See full target information ITIH4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Analytical cellular pathology (Amsterdam) 2021:7852710 PubMed34540569

2021

Endoplasmic Reticulum Stress Is Associated with the Mesencephalic Dopaminergic Neuron Injury in Stressed Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Shiba Niu,Weibo Shi,Yingmin Li,Shanyong Yi,Yang Li,Xia Liu,Bin Cong,Guanglong He

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ITIH4 antibody [EPR11233(2)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] - BSA and Azide free (AB240221)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] - BSA and Azide free (AB240221)

Immunoprecipitation - Anti-ITIH4 antibody [EPR11233(2)] - BSA and Azide free (AB240221)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise