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AB308352

Anti-ITK antibody [HL1264]

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Rabbit Recombinant Monoclonal ITK/EMT antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ITK.

View Alternative Names

EMT, LYK, ITK, Tyrosine-protein kinase ITK/TSK, Interleukin-2-inducible T-cell kinase, Kinase EMT, T-cell-specific kinase, Tyrosine-protein kinase Lyk, IL-2-inducible T-cell kinase

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-ITK antibody [HL1264] (AB308352)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ITK antibody [HL1264] (AB308352)

For immunofluorescence analysis, Jurkat cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of ITK protein using ab308352 at 1/500 dilution (green). Nuclei (blue) is stained with fluoroshield with DAPI.

Immunohistochemistry paraffin embedded sections - Anti-ITK antibody [HL1264] (AB308352)
  • IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-ITK antibody [HL1264] (AB308352)

Immunohistochemical analysis of mouse kidney tissue labeling ITK using ab308352 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Immunohistochemistry paraffin embedded sections - Anti-ITK antibody [HL1264] (AB308352)
  • IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-ITK antibody [HL1264] (AB308352)

Immunohistochemical analysis of mouse lymph node tissue labeling ITK using ab308352 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Western blot - Anti-ITK antibody [HL1264] (AB308352)
  • WB

Supplier Data

Western blot - Anti-ITK antibody [HL1264] (AB308352)

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ITK antibody [HL1264] (ab308352) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-ITK antibody [HL1264] (ab308352) at 1/1000 dilution

Lane 1:

THP-1 cell lysate at 30 µg

Lane 2:

Jurkat cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 71.8 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1264

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ITK. The exact immunogen used to generate this antibody is proprietary information.

Q08881

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ITK also known as Interleukin-2-inducible T-cell kinase is a protein belonging to the Tec family of non-receptor tyrosine kinases. It weighs approximately 72 kDa and plays a significant role in signal transduction in immune cells specifically T cells. ITK is widely expressed in lymphoid tissues particularly in thymocytes and peripheral T cells. ITK products often include variants like ITK-1 and ITK kit which are useful in various scientific applications.
Biological function summary

ITK is integral to the activation and development of T cells which are critical components of the adaptive immune response. ITK functions as part of a larger signaling complex interacting with proteins such as LAT and SLP-76 to mediate downstream signaling events following T-cell receptor engagement. This interaction facilitates the phosphorylation of multiple substrates which is necessary for T-cell proliferation differentiation and cytokine production.

Pathways

ITK plays an important role in T-cell receptor signaling and the downstream Ras/MAPK pathway. It interacts with proteins like ZAP-70 and LCK in these pathways which are important for initiating T-cell activation and function. The modulation of these pathways by ITK is essential for proper immune function and response to antigens.

ITK is linked to immunodeficiency and autoimmune disorders. Dysregulation of ITK activity can lead to conditions like X-linked lymphoproliferative syndrome and systemic lupus erythematosus. The EMT protein also ties with ITK concerning certain neoplastic conditions where aberrant signaling contributes to the pathogenesis. Researchers study ITK's interactions with other proteins such as BSA EMT to understand its role in these diseases better and explore potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins : the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation (PubMed : 12186560, PubMed : 12682224, PubMed : 21725281). Required for TCR-mediated calcium response in gamma-delta T-cells, may also be involved in the modulation of the transcriptomic signature in the Vgamma2-positive subset of immature gamma-delta T-cells (By similarity). Phosphorylates TBX21 at 'Tyr-530' and mediates its interaction with GATA3 (By similarity).
See full target information ITK
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Product promise

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