AB32507

Anti-ITK/EMT antibody [Y402]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal ITK/EMT antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

EMT, LYK, ITK, Tyrosine-protein kinase ITK/TSK, Interleukin-2-inducible T-cell kinase, Kinase EMT, T-cell-specific kinase, Tyrosine-protein kinase Lyk, IL-2-inducible T-cell kinase

3 Images

Lab

Immunoprecipitation - Anti-ITK/EMT antibody [Y402] (AB32507)

ab32507 (purified) at 1 : 20 dilution (1.0µg) immunoprecipitating ITK/EMT in Jurkat whole cell lysate.
Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-ITK/EMT antibody [Y402] (ab32507) at 1/20 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 10 µg

Lane 2:

ab32507 & Jurkat whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/smarcc1-baf155-antibody-epr12395-chip-grade-ab172638'>ab172638</a> in Jurkat whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 72 kDa

false

Unknown

Western blot - Anti-ITK/EMT antibody [Y402] (AB32507)

All lanes:

Western blot - Anti-ITK/EMT antibody [Y402] (ab32507) at 1/1000 dilution

All lanes:

Jurkat cell lysate

Predicted band size: 72 kDa

Observed band size: 72 kDa

false

Lab

Western blot - Anti-ITK/EMT antibody [Y402] (AB32507)

Blocking and diluting buffer : 5% NFDM/TBST

Lane 1:

Western blot - Anti-ITK/EMT antibody [Y402] (ab32507) at 1/2000 dilution

Lane 2:

Western blot - Anti-ITK/EMT antibody [Y402] (ab32507) at 1/10000 dilution

All lanes:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 72 kDa

false

Carrier free

Anti-ITK/EMT antibody [Y402] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

Y402

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20", "IP-species-notes": "For unpurified use at 1/50.", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "For unpurified use at 1/1000.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ITK also known as Interleukin-2-inducible T-cell kinase is a protein belonging to the Tec family of non-receptor tyrosine kinases. It weighs approximately 72 kDa and plays a significant role in signal transduction in immune cells specifically T cells. ITK is widely expressed in lymphoid tissues particularly in thymocytes and peripheral T cells. ITK products often include variants like ITK-1 and ITK kit which are useful in various scientific applications.

Biological function summary

ITK is integral to the activation and development of T cells which are critical components of the adaptive immune response. ITK functions as part of a larger signaling complex interacting with proteins such as LAT and SLP-76 to mediate downstream signaling events following T-cell receptor engagement. This interaction facilitates the phosphorylation of multiple substrates which is necessary for T-cell proliferation differentiation and cytokine production.

Pathways

ITK plays an important role in T-cell receptor signaling and the downstream Ras/MAPK pathway. It interacts with proteins like ZAP-70 and LCK in these pathways which are important for initiating T-cell activation and function. The modulation of these pathways by ITK is essential for proper immune function and response to antigens.

ITK is linked to immunodeficiency and autoimmune disorders. Dysregulation of ITK activity can lead to conditions like X-linked lymphoproliferative syndrome and systemic lupus erythematosus. The EMT protein also ties with ITK concerning certain neoplastic conditions where aberrant signaling contributes to the pathogenesis. Researchers study ITK's interactions with other proteins such as BSA EMT to understand its role in these diseases better and explore potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins : the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation (PubMed : 12186560, PubMed : 12682224, PubMed : 21725281). Required for TCR-mediated calcium response in gamma-delta T-cells, may also be involved in the modulation of the transcriptomic signature in the Vgamma2-positive subset of immature gamma-delta T-cells (By similarity). Phosphorylates TBX21 at 'Tyr-530' and mediates its interaction with GATA3 (By similarity).

See full target information ITK

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Clinical cancer research : an official journal of the American Association for Cancer Research 23:3734-3743 PubMed28034907

2016

Comparison of Acalabrutinib, A Selective Bruton Tyrosine Kinase Inhibitor, with Ibrutinib in Chronic Lymphocytic Leukemia Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Viralkumar Patel,Kumudha Balakrishnan,Elena Bibikova,Mary Ayres,Michael J Keating,William G Wierda,Varsha Gandhi

The Annals of thoracic surgery 100:1817-24; discussion 1824-5 PubMed26298168

2015

Triptolide Inhibits Lung Cancer Cell Migration, Invasion, and Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Theresa A Reno,Jae Y Kim,Dan J Raz

The Journal of clinical investigation 119:1350-8 PubMed19425169

2009

Girls homozygous for an IL-2-inducible T cell kinase mutation that leads to protein deficiency develop fatal EBV-associated lymphoproliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Kirsten Huck,Oliver Feyen,Tim Niehues,Franz Rüschendorf,Norbert Hübner,Hans-Jürgen Laws,Tanja Telieps,Stefan Knapp,Hans-Heinrich Wacker,Alfons Meindl,Hassan Jumaa,Arndt Borkhardt

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ITK/EMT antibody [Y402]

Immunoprecipitation - Anti-ITK/EMT antibody [Y402] (AB32507)

Western blot - Anti-ITK/EMT antibody [Y402] (AB32507)

Western blot - Anti-ITK/EMT antibody [Y402] (AB32507)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise