JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB157802

Anti-ITK/EMT antibody [Y402] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal ITK/EMT antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

View Alternative Names

EMT, LYK, ITK, Tyrosine-protein kinase ITK/TSK, Interleukin-2-inducible T-cell kinase, Kinase EMT, T-cell-specific kinase, Tyrosine-protein kinase Lyk, IL-2-inducible T-cell kinase

3 Images
Immunoprecipitation - Anti-ITK/EMT antibody [Y402] - BSA and Azide free (AB157802)
  • IP

Lab

Immunoprecipitation - Anti-ITK/EMT antibody [Y402] - BSA and Azide free (AB157802)

This data was developed using ab32507, the same antibody clone in a different buffer formulation.

ab32507 (purified) at 1 : 20 dilution (1.0µg) immunoprecipitating ITK/EMT in Jurkat whole cell lysate.
Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-ITK/EMT antibody [Y402] (<a href='/en-us/products/primary-antibodies/itk-emt-antibody-y402-ab32507'>ab32507</a>) at 1/20 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 10 µg

Lane 2:

<a href='/en-us/products/primary-antibodies/itk-emt-antibody-y402-ab32507'>ab32507</a> & Jurkat whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/smarcc1-baf155-antibody-epr12395-chip-grade-ab172638'>ab172638</a> in Jurkat whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 72 kDa

false

Western blot - Anti-ITK/EMT antibody [Y402] - BSA and Azide free (AB157802)
  • WB

Unknown

Western blot - Anti-ITK/EMT antibody [Y402] - BSA and Azide free (AB157802)

This data was developed using ab32507, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ITK/EMT antibody [Y402] (<a href='/en-us/products/primary-antibodies/itk-emt-antibody-y402-ab32507'>ab32507</a>) at 1/1000 dilution

All lanes:

Jurkat cell lysate

Predicted band size: 72 kDa

Observed band size: 72 kDa

false

Western blot - Anti-ITK/EMT antibody [Y402] - BSA and Azide free (AB157802)
  • WB

Lab

Western blot - Anti-ITK/EMT antibody [Y402] - BSA and Azide free (AB157802)

This data was developed using ab32507, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer : 5% NFDM/TBST

Lane 1:

Western blot - Anti-ITK/EMT antibody [Y402] (<a href='/en-us/products/primary-antibodies/itk-emt-antibody-y402-ab32507'>ab32507</a>) at 1/2000 dilution

Lane 2:

Western blot - Anti-ITK/EMT antibody [Y402] (<a href='/en-us/products/primary-antibodies/itk-emt-antibody-y402-ab32507'>ab32507</a>) at 1/10000 dilution

All lanes:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 72 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

Y402

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

ab157802 is the carrier-free version of ab32507.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ITK also known as Interleukin-2-inducible T-cell kinase is a protein belonging to the Tec family of non-receptor tyrosine kinases. It weighs approximately 72 kDa and plays a significant role in signal transduction in immune cells specifically T cells. ITK is widely expressed in lymphoid tissues particularly in thymocytes and peripheral T cells. ITK products often include variants like ITK-1 and ITK kit which are useful in various scientific applications.
Biological function summary

ITK is integral to the activation and development of T cells which are critical components of the adaptive immune response. ITK functions as part of a larger signaling complex interacting with proteins such as LAT and SLP-76 to mediate downstream signaling events following T-cell receptor engagement. This interaction facilitates the phosphorylation of multiple substrates which is necessary for T-cell proliferation differentiation and cytokine production.

Pathways

ITK plays an important role in T-cell receptor signaling and the downstream Ras/MAPK pathway. It interacts with proteins like ZAP-70 and LCK in these pathways which are important for initiating T-cell activation and function. The modulation of these pathways by ITK is essential for proper immune function and response to antigens.

ITK is linked to immunodeficiency and autoimmune disorders. Dysregulation of ITK activity can lead to conditions like X-linked lymphoproliferative syndrome and systemic lupus erythematosus. The EMT protein also ties with ITK concerning certain neoplastic conditions where aberrant signaling contributes to the pathogenesis. Researchers study ITK's interactions with other proteins such as BSA EMT to understand its role in these diseases better and explore potential therapeutic targets.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins : the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation (PubMed : 12186560, PubMed : 12682224, PubMed : 21725281). Required for TCR-mediated calcium response in gamma-delta T-cells, may also be involved in the modulation of the transcriptomic signature in the Vgamma2-positive subset of immature gamma-delta T-cells (By similarity). Phosphorylates TBX21 at 'Tyr-530' and mediates its interaction with GATA3 (By similarity).
See full target information ITK
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB45141

Anti-JAK3 antibody [EP909Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-JAK3 antibody [EP909Y] (AB45141)

  • 5
  • 1
Primary Antibodies

AB208937

Anti-BTK antibody [EPR20445]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTK antibody [EPR20445] (AB208937)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB32368

Anti-Tec antibody [Y398]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Tec antibody [Y398] (AB32368)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com