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Rabbit Recombinant Monoclonal ITLN1 antibody. Carrier free. Suitable for WB, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (AB251518), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (AB251518), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (AB251518), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (AB251518), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (AB251518), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-FrIHC-P
Human
Tested
Expected
Expected
Mouse
Expected
Tested
Expected
Rat
Expected
Expected
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).

Expected
Expected

Species
Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Human, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Lectin that specifically recognizes microbial carbohydrate chains in a calcium-dependent manner (PubMed:11313366, PubMed:26148048). Binds to microbial glycans that contain a terminal acyclic 1,2-diol moiety, including beta-linked D-galactofuranose (beta-Galf), D-phosphoglycerol-modified glycans, D-glycero-D-talo-oct-2-ulosonic acid (KO) and 3-deoxy-D-manno-oct-2-ulosonic acid (KDO) (PubMed:26148048). Binds to glycans from Gram-positive and Gram-negative bacteria, including K.pneumoniae, S.pneumoniae, Y.pestis, P.mirabilis and P.vulgaris (PubMed:26148048). Does not bind human glycans (PubMed:26148048). Probably plays a role in the defense system against microorganisms (Probable). May function as adipokine that has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes (PubMed:16531507). Increases AKT phosphorylation in the absence and presence of insulin (PubMed:16531507). May interact with lactoferrin/LTF and increase its uptake, and may thereby play a role in iron absorption (PubMed:11747454, PubMed:23921499).

Additional Targets

ITLN2

Alternative names

INTL, ITLN, LFR, UNQ640/PRO1270, ITLN1, Intelectin-1, ITLN-1, Endothelial lectin HL-1, Galactofuranose-binding lectin, Intestinal lactoferrin receptor, Omentin

Recommended products

Rabbit Recombinant Monoclonal ITLN1 antibody. Carrier free. Suitable for WB, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR20311
Purification technique
Affinity purification Protein A
Specificity

ITLN1 mRNA expression in stomach has been reported [PMID: 19549487]. Another report [PMID: 22768319] shows no staining on stomach at protein level. In our hands this antibody showed weak cytoplasmic staining on stomach samples.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251518 is the carrier-free version of Anti-ITLN1 + ITLN2 antibody [EPR20311] ab210483.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ITLN1 and ITLN2 also known as intelectin-1 and intelectin-2 are carbohydrate-binding proteins. ITLN1 has a molecular mass approximately 35 kDa while ITLN2 is similar in size. Both proteins are mainly expressed in human adipose tissue small intestine and the respiratory tract. These proteins have a significant presence in epithelial cells and are secreted as part of the extracellular matrix. They belong to the lectin family and recognize galactofuranose-containing glycans in microorganisms.

Biological function summary

Intelectins modulate immune responses and have been identified in promoting microbial defense. They are known for their role in binding specific sugars and facilitating the neutralization of pathogens. ITLN1 and ITLN2 do not form a complex but share functional similarities in immune surveillance. Their activity contributes to interactions with lipid metabolism and insulin signaling influencing glucose homeostasis and inflammation regulation.

Pathways

Intelectins integrate with the immune response pathway and insulin signaling pathway. They influence immune system activation by recognizing carbohydrate structures unique to microorganisms. The proteins interact with immune molecules like complement proteins and play supportive roles in the insulin signaling cascade linking them to proteins such as GLUT4 and IRS-1. Their functions promote the maintenance of metabolic balance and protect against microbial infections.

Associated diseases and disorders

ITLN1 and ITLN2 play roles in metabolic diseases and respiratory infections. In obesity dysregulation of ITLN1 links to altered insulin sensitivity associating the protein with adiponectin. In asthma differential expression of these proteins relates to inflammation and airway resistance connecting ITLN2 to interleukins. These associations highlight intelectins as potential biomarkers or therapeutic targets in metabolic and inflammatory conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518), expandable thumbnail

    Western blot - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)

    This data was developed using Anti-ITLN1 + ITLN2 antibody [EPR20311] ab210483, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (Anti-ITLN1 + ITLN2 antibody [EPR20311] ab210483) at 1/1000 dilution

    Lane 1: Recombinant human ITLN1 protein (ab109151) at 0.01 µg

    Lane 2: Human ITLN2 His-tagged recombinant protein (aa27-325) at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa

    Observed band size: 36 kDa, 37 kDa

    Exposure time: 10s

  • Immunohistochemistry (Frozen sections) - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)

    This data was developed using Anti-ITLN1 + ITLN2 antibody [EPR20311] ab210483, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse colon tissue labeling ITLN1 + ITLN2 with Anti-ITLN1 + ITLN2 antibody [EPR20311] ab210483 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on goblet cells of mouse colon is observed [PMID: 22768319] [PMID: 19549487]. The nuclear counterstain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)

    This data was developed using Anti-ITLN1 + ITLN2 antibody [EPR20311] ab210483, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human colon tissue labeling ITLN1 + ITLN2 with Anti-ITLN1 + ITLN2 antibody [EPR20311] ab210483 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasmic staining on goblet cells of human colon is observed [PMID: 22768319] [PMID: 19549487]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)

    This data was developed using Anti-ITLN1 + ITLN2 antibody [EPR20311] ab210483, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling ITLN1 + ITLN2 with Anti-ITLN1 + ITLN2 antibody [EPR20311] ab210483 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasmic staining on goblet cells of mouse colon is observed [PMID: 22768319] [PMID: 19549487]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)

    This data was developed using Anti-ITLN1 + ITLN2 antibody [EPR20311] ab210483, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling ITLN1 + ITLN2 with Anti-ITLN1 + ITLN2 antibody [EPR20311] ab210483 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Cytoplasmic staining on goblet cells of rat colon is observed [PMID: 22768319] [PMID: 19549487]. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518), expandable thumbnail

    Western blot - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)

    This data was developed using Anti-ITLN1 + ITLN2 antibody [EPR20311] ab210483, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Spleen is a negative control (PMID: 11181563).

    All lanes: Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (Anti-ITLN1 + ITLN2 antibody [EPR20311] ab210483) at 1/5000 dilution

    Lane 1: Human colon lysate at 20 µg

    Lane 2: Human fetal spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa

    Observed band size: 40 kDa

    Exposure time: 5s

  • Western blot - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518), expandable thumbnail

    Western blot - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)

    This data was developed using Anti-ITLN1 + ITLN2 antibody [EPR20311] ab210483, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST. Exposure times: Lanes 1-2: 1 minute; Lanes 3-4: 30 seconds.

    The observed molecular weight is consistent with the literature (PMID: 11313366, 20628387).

    All lanes: Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (Anti-ITLN1 + ITLN2 antibody [EPR20311] ab210483) at 1/5000 dilution

    Lane 1: Mouse colon lysate at 20 µg

    Lane 2: Rat small intestine lysate at 20 µg

    Lane 3: Mouse small intestine lysate at 20 µg

    Lane 4: Rat colon lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa

    Observed band size: 40 kDa

  • Western blot - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518), expandable thumbnail

    Western blot - Anti-ITLN1+ITLN2 antibody [EPR20311] - BSA and Azide free (ab251518)

    This data was developed using Anti-ITLN1 + ITLN2 antibody [EPR20311] ab210483, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The observed molecular weight is consistent with the literature (PMID: 11313366 and 20628387).

    All lanes: Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (Anti-ITLN1 + ITLN2 antibody [EPR20311] ab210483) at 1/5000 dilution

    All lanes: Human small intestine lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 35 kDa

    Observed band size: 40 kDa

    Exposure time: 1s

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Product protocols

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