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AB203620

Anti-ITM2A antibody

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(1 Publication)

Rabbit Polyclonal ITM2A antibody. Suitable for IHC-P, ICC/IF and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ITM2A aa 50-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

UNQ603/PRO1189, ITM2A, Integral membrane protein 2A, Protein E25

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITM2A antibody (AB203620)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITM2A antibody (AB203620)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Rat brain tissue labeling ITM2A with ab203620 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

Immunocytochemistry/ Immunofluorescence - Anti-ITM2A antibody (AB203620)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ITM2A antibody (AB203620)

Immunofluorescent analysis of 4% PFA fixed rat brain vascular endothelial cells (RBE4) labeling ITM2A with ab203620 at 1/100 dilution, followed by conjugated secondary antibody(green) and DAPI staining (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITM2A antibody (AB203620)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITM2A antibody (AB203620)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling ITM2A with ab203620 at 1/200 dilution overnight at 4°C followed by Goat Anti-Rabbit IgG, Cy3 at 1/200 dilution for 40 minutes at 37°C.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human ITM2A aa 50-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O43736

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe the recommended dilution is 1/50 – 1/200.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The integral membrane protein 2A (ITM2A) also known as E25B and BRICD2B is a transmembrane protein with a molecular weight of approximately 30 kDa. It plays a mechanical role in protein processing and transport within cells. ITM2A is mainly expressed in the brain cartilage and lymphocytes. The expression is particularly notable during the early stages of lymphocyte development and in cartilage during early growth phases.
Biological function summary

ITM2A influences cellular development processes by modulating cell proliferation and differentiation. It does not form a part of any larger complex but acts independently to affect the maturation of cells particularly in tissues where it is expressed. ITM2A impacts various cellular pathways influencing how cells transition from one stage of development to the next and is significant in normal tissue development and function.

Pathways

ITM2A interacts within the BMP signaling pathway and influences cell growth and differentiation. BMP signaling known for its role in cartilage and bone formation finds ITM2A as an important component in modulating these processes. ITM2A is related to the BMP family of proteins impacting how these signaling cascades unfold particularly in developmental stages guiding cellular responses in these pathways.

Altered expression of ITM2A has been associated with cartilage-related conditions such as osteoarthritis and certain lymphocyte-linked disorders. ITM2A ties with disease progression by impacting protein interactions relevant to these conditions. While involved in osteoarthritis it connects with matrix metalloproteinase (MMP) proteins which play a role in cartilage degradation hinting at its involvement in cartilage homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information ITM2A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Critical reviews in eukaryotic gene expression 32:11-20 PubMed35997114

2022

LncRNA PCED1B-AS1 Upregulation in Hepatocellular Carcinoma and Regulation of the miR-10a/BCL6 Axis to Promote Cell Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Haitao Ding,Juan He,Weili Xiao,Zhihong Ren,Wanting Gao
View all publications
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