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AB150420

Anti-ITPA antibody [EPR8779]

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(1 Publication)

Rabbit Recombinant Monoclonal ITPA antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C20orf37, My049, OK/SW-cl.9, ITPA, Inosine triphosphate pyrophosphatase, ITPase, Inosine triphosphatase, Non-canonical purine NTP pyrophosphatase, Non-standard purine NTP pyrophosphatase, Nucleoside-triphosphate diphosphatase, Nucleoside-triphosphate pyrophosphatase, Putative oncogene protein hlc14-06-p, NTPase

2 Images
Western blot - Anti-ITPA antibody [EPR8779] (AB150420)
  • WB

Lab

Western blot - Anti-ITPA antibody [EPR8779] (AB150420)

Lanes 1-3 : Merged signal (red and green). Green - ab150420 observed at 21 kDa. Red - loading control ab8245 observed at 36 kDa.

ab150420 Anti-ITPA antibody [EPR8779] was shown to specifically react with ITPA in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266679 (knockout cell lysate ab258477) was used. Wild-type and ITPA knockout samples were subjected to SDS-PAGE. ab150420 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ITPA antibody [EPR8779] (ab150420) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

ITPA knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human ITPA knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-itpa-knockout-hek-293t-cell-line-ab266679'>ab266679</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

Western blot - Anti-ITPA antibody [EPR8779] (AB150420)
  • WB

Unknown

Western blot - Anti-ITPA antibody [EPR8779] (AB150420)

All lanes:

Western blot - Anti-ITPA antibody [EPR8779] (ab150420) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8779

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/10 - 1/100", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ITPA also known as inosine triphosphate pyrophosphatase is an important enzyme responsible for catalyzing the hydrolysis of inosine triphosphate (ITP) to inosine monophosphate (IMP). This enzyme prevents the accumulation of non-canonical nucleotides in the cell. ITPA has a mass of approximately 21 kDa and is expressed ubiquitously in various tissues indicating its importance across different cell types. The enzyme is also present in HEK 293T cells as researchers often use these cells for studies due to their growth characteristics and transfection efficiency.
Biological function summary

The enzyme plays a pivotal role in nucleotide metabolism by regulating the levels of ITP deoxyinosine triphosphate (dITP) and xanthosine triphosphate (XTP). By hydrolyzing these non-standard nucleotides ITPA maintains genomic integrity and prevents mutations during DNA replication. It is not typically considered part of a larger complex but acts independently to safeguard the nucleotide pool from potentially harmful nucleotide analogs.

Pathways

ITPA's activity is essential in the purine metabolism pathway where it helps maintain a proper balance of nucleotide synthesis and degradation. This function helps prevent the incorporation of non-standard bases into DNA and RNA which can lead to genetic instability. ITPA activity is also intertwined with the salvage pathway of purine nucleotides. This connection highlights its indirect relationship with proteins involved in nucleotide synthesis and repair such as DNA polymerases and other nucleotide-metabolizing enzymes.

ITPA has associations with certain pathological conditions. For example ITPA deficiency can lead to inosine triphosphate accumulation which might contribute to hemolytic anemia. Mutations in the ITPA gene can also impact drug metabolism notably in patients receiving thiopurine drugs such as those used for leukemia treatment. These mutations may lead to adverse drug reactions due to the altered nucleotide balance. The connection with these conditions emphasizes the role of ITPA in maintaining cellular homeostasis and its potential influence on pharmacogenomics.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pyrophosphatase that hydrolyzes the non-canonical purine nucleotides inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triphosphate (dHAPTP) and xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.
See full target information ITPA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Progress in neurobiology 238:102632 PubMed38821345

2024

A molecularly defined orbitofrontal cortical neuron population controls compulsive-like behavior, but not inflexible choice or habit.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie T Yount,Silu Wang,Aylet T Allen,Lauren P Shapiro,Laura M Butkovich,Shannon L Gourley
View all publications
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