Rabbit Polyclonal ITPKA antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Inositol-trisphosphate 3-kinase A aa 300-400.
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Publications
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- Molecular genetics & genomic medicine 9:e15982021Relationship of ITPKA expression with the prognosis of breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Zhang,Sujie Zhang,Xiaoyan Li,Hongying PiPubMed 33624455
- OncoTargets and therapy 13:10515-105232020ITPKA1 Promotes Growth, Migration and Invasion of Renal Cell Carcinoma via Activation of mTOR Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Zhu et. al.PubMed 33116630
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Inositol-trisphosphate 3-kinase A aa 300-400. The exact immunogen used to generate this antibody is proprietary information. Database link P23677
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2500.00000 - 1/5000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/2500.00000 - 1/5000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Catalyzes the phosphorylation of 1D-myo-inositol 1,4,5-trisphosphate (InsP3) into 1D-myo-inositol 1,3,4,5-tetrakisphosphate and participates to the regulation of calcium homeostasis.
Alternative names
Inositol-trisphosphate 3-kinase A, IP3 3-kinase A, IP3K A, InsP 3-kinase A, ITPKA
Recommended products
Rabbit Polyclonal ITPKA antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Inositol-trisphosphate 3-kinase A aa 300-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Inositol-trisphosphate 3-kinase A aa 300-400. The exact immunogen used to generate this antibody is proprietary information. Database link P23677
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ITPKA or inositol-trisphosphate 3-kinase A is an enzyme known for adding a phosphate group to inositol 145-trisphosphate (IP3) forming inositol 1345-tetrakisphosphate (IP4). The protein weighs approximately 51 kDa and plays an important role in regulating calcium signaling within cells. ITPKA expression is highly found in the brain where it influences various neuronal functions. ITPKA has an important role in the nervous system due to its enzyme activity.
- Biological function summary
ITPKA participates in modifying phosphoinositide signaling by altering IP3 and IP4 levels. This protein does not form part of a larger complex but acts independently to influence calcium released from intracellular stores. By altering levels of IP3 ITPKA indirectly influences cellular activities like synaptic plasticity and neuronal excitability. Its kinase activity has links to other cellular functions which are less well-defined but suggest involvement in broader cellular processes.
- Pathways
ITPKA participates in the inositol phosphate metabolism and postsynaptic density pathways. It plays an essential part in modulating the release of calcium through its interaction with IP3 important for various downstream effects. ITPKA has associations with proteins such as IP3 receptors due to its enzymatic products. This interaction is also important for further downstream effects in signaling pathways affecting cellular functions like synaptic strength and plasticity.
- Associated diseases and disorders
ITPKA has associations with neuropsychiatric disorders and cancer. Aberrant ITPKA levels have links to psychiatric disorders due to impacts on neuronal signaling and plasticity. In cancer ITPKA may influence cell proliferation and migration potentially through its downstream effect on calcium signals. Other proteins such as phospholipase C connect to ITPKA activity in pathways altered in these conditions highlighting the broader impact of its enzymatic activity in disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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9 product images
Immunocytochemistry/ Immunofluorescence - Anti-ITPKA antibody (ab251867)
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for ITPKA (green) using ab251867 at 4 µg/ml in ICC/IF.
Western blot - Anti-ITPKA antibody (ab251867)
Western blot analysis of human cerebral cortex tissue lysate labeling ITPKA with ab251867 at 0.04 ug/ml.
All lanes: Western blot - Anti-ITPKA antibody (ab251867) at 0.04 µg/mL
All lanes: Human cerebral cortex tissue lysate
Predicted band size: 51 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPKA antibody (ab251867)
Formalin-fixed, paraffin-embedded mouse hypothalamus tissue stained for ITPKA with ab251867 at a 1/2500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPKA antibody (ab251867)
Paraffin-embedded human cerebral cortex tissue stained for ITPKA using ab251867 at 1/2500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPKA antibody (ab251867)
Paraffin-embedded mouse subiculum tissue stained for ITPKA using ab251867 at 1/2500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPKA antibody (ab251867)
Paraffin-embedded human glioma tissue stained for ITPKA using ab251867 at 1/2500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPKA antibody (ab251867)
Paraffin-embedded human caudate tissue stained for ITPKA using ab251867 at 1/2500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPKA antibody (ab251867)
Paraffin-embedded human liver tissue shows absence of immunoreactivity for ITPKA when incubated with ab251867 at 1/2500 dilution (negative control).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPKA antibody (ab251867)
Paraffin-embedded mouse brain tissue stained for ITPKA using ab251867 at 1/2500 dilution in immunohistochemical analysis.
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