AB251867

Anti-ITPKA antibody

Name: Anti-ITPKA antibody (ab251867) | Abcam
Brand: Abcam Limited
SKU: AB251867
Price: 655 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal ITPKA antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Inositol-trisphosphate 3-kinase A aa 300-400.

View Alternative Names

Inositol-trisphosphate 3-kinase A, IP3 3-kinase A, IP3K A, InsP 3-kinase A, ITPKA

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPKA antibody (AB251867)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPKA antibody (AB251867)

Paraffin-embedded human liver tissue shows absence of immunoreactivity for ITPKA when incubated with ab251867 at 1/2500 dilution (negative control).

Immunocytochemistry/ Immunofluorescence - Anti-ITPKA antibody (AB251867)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ITPKA antibody (AB251867)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for ITPKA (green) using ab251867 at 4 µg/ml in ICC/IF.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPKA antibody (AB251867)

Paraffin-embedded human cerebral cortex tissue stained for ITPKA using ab251867 at 1/2500 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPKA antibody (AB251867)

Paraffin-embedded mouse brain tissue stained for ITPKA using ab251867 at 1/2500 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPKA antibody (AB251867)

Paraffin-embedded mouse subiculum tissue stained for ITPKA using ab251867 at 1/2500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-ITPKA antibody (AB251867)

Western blot analysis of human cerebral cortex tissue lysate labeling ITPKA with ab251867 at 0.04 ug/ml.

All lanes:

Western blot - Anti-ITPKA antibody (ab251867) at 0.04 µg/mL

All lanes:

Human cerebral cortex tissue lysate

Predicted band size: 51 kDa

false

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPKA antibody (AB251867)

Paraffin-embedded human glioma tissue stained for ITPKA using ab251867 at 1/2500 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPKA antibody (AB251867)

Paraffin-embedded human caudate tissue stained for ITPKA using ab251867 at 1/2500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPKA antibody (AB251867)

Formalin-fixed, paraffin-embedded mouse hypothalamus tissue stained for ITPKA with ab251867 at a 1/2500 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Inositol-trisphosphate 3-kinase A aa 300-400. The exact immunogen used to generate this antibody is proprietary information.

P23677

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2500 - 1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2500 - 1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ITPKA or inositol-trisphosphate 3-kinase A is an enzyme known for adding a phosphate group to inositol 145-trisphosphate (IP3) forming inositol 1345-tetrakisphosphate (IP4). The protein weighs approximately 51 kDa and plays an important role in regulating calcium signaling within cells. ITPKA expression is highly found in the brain where it influences various neuronal functions. ITPKA has an important role in the nervous system due to its enzyme activity.

Biological function summary

ITPKA participates in modifying phosphoinositide signaling by altering IP3 and IP4 levels. This protein does not form part of a larger complex but acts independently to influence calcium released from intracellular stores. By altering levels of IP3 ITPKA indirectly influences cellular activities like synaptic plasticity and neuronal excitability. Its kinase activity has links to other cellular functions which are less well-defined but suggest involvement in broader cellular processes.

Pathways

ITPKA participates in the inositol phosphate metabolism and postsynaptic density pathways. It plays an essential part in modulating the release of calcium through its interaction with IP3 important for various downstream effects. ITPKA has associations with proteins such as IP3 receptors due to its enzymatic products. This interaction is also important for further downstream effects in signaling pathways affecting cellular functions like synaptic strength and plasticity.

ITPKA has associations with neuropsychiatric disorders and cancer. Aberrant ITPKA levels have links to psychiatric disorders due to impacts on neuronal signaling and plasticity. In cancer ITPKA may influence cell proliferation and migration potentially through its downstream effect on calcium signals. Other proteins such as phospholipase C connect to ITPKA activity in pathways altered in these conditions highlighting the broader impact of its enzymatic activity in disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the phosphorylation of 1D-myo-inositol 1,4,5-trisphosphate (InsP3) into 1D-myo-inositol 1,3,4,5-tetrakisphosphate and participates to the regulation of calcium homeostasis.

See full target information Inositol-trisphosphate 3-kinase A

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e35303 PubMed39170313

2024

ITPKA phosphorylates PYCR1 and promotes the progression of glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangying Luo,Tao Chen,Junyi Deng,Ziyuan Liu,Changlong Bi,Song Lan

Frontiers in oncology 11:792285 PubMed35047403

2022

Mediator Complex Subunit 19 Promotes the Development of Hepatocellular Carcinoma by Regulating the AKT/mTOR Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuting Zhang,Peifang Qin,Xingfeng Xu,Mao Li,Haitao Huang,Jianguo Yan,Yali Zhou

Molecular genetics & genomic medicine 9:e1598 PubMed33624455

2021

Relationship of ITPKA expression with the prognosis of breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhang,Sujie Zhang,Xiaoyan Li,Hongying Pi

OncoTargets and therapy 13:10515-10523 PubMed33116630

2020

ITPKA1 Promotes Growth, Migration and Invasion of Renal Cell Carcinoma via Activation of mTOR Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Zhu,An Xu,Yang Zhang,Nan Huo,Rui Cong,Luyuan Ma,Zhong Chu,Zhi Tang,Xiaofeng Kang,Shaozhong Xian,Xiaojie Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ITPKA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPKA antibody (AB251867)

Immunocytochemistry/ Immunofluorescence - Anti-ITPKA antibody (AB251867)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPKA antibody (AB251867)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPKA antibody (AB251867)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPKA antibody (AB251867)

Western blot - Anti-ITPKA antibody (AB251867)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPKA antibody (AB251867)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPKA antibody (AB251867)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPKA antibody (AB251867)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise