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AB188007

Anti-ITPKC antibody

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(1 Publication)

Rabbit Polyclonal ITPKC antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ITPKC aa 250-400.

View Alternative Names

IP3KC, ITPKC, Inositol-trisphosphate 3-kinase C, IP3 3-kinase C, IP3K C, InsP 3-kinase C

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ITPKC antibody (AB188007)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ITPKC antibody (AB188007)

Immunocytochemistry/immunofluorescent analysis of U-2OS cells staining ITPKC antibody with ab18807 at 1μg/ml (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPKC antibody (AB188007)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPKC antibody (AB188007)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling ITPKC with ab188007 at 1/1000 dilution.

Western blot - Anti-ITPKC antibody (AB188007)
  • WB

Supplier Data

Western blot - Anti-ITPKC antibody (AB188007)

All lanes:

Western blot - Anti-ITPKC antibody (ab188007) at 1/500 dilution

Lane 1:

RT4 cell lysate

Lane 2:

U-251MG sp cell lysate

Predicted band size: 75 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ITPKC aa 250-400. The exact immunogen used to generate this antibody is proprietary information.

Q96DU7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ITPKC also known as inositol 145-trisphosphate 3-kinase C is an enzyme involved in the phosphorylation of inositol 145-trisphosphate (IP3) converting it into inositol 1345-tetrakisphosphate (IP4). This protein has a molecular mass of approximately 55 kDa and is expressed in various tissues including the heart brain and skeletal muscle. Its role in cellular signaling processes makes it an important target for research in multiple biological functions.
Biological function summary

The enzyme ITPKC plays an important role in calcium signaling by modulating intracellular calcium levels. It does not typically form a complex with other proteins but acts independently to regulate IP3 a secondary messenger critical for calcium release from the endoplasmic reticulum. This regulation affects processes such as muscle contraction cell growth and apoptosis. The enzyme's function in maintaining cellular calcium levels highlights its importance in various physiological contexts.

Pathways

ITPKC operates within the phosphoinositide pathway playing an important role in calcium signaling and homeostasis. The regulation of IP3 and IP4 levels by ITPKC impacts signal transduction pathways influencing activities like cell proliferation and immune responses. Key interactions occur with proteins such as IP3 receptors on the endoplasmic reticulum which facilitate calcium release and uptake into cellular compartments.

ITPKC influences the development of certain conditions such as Kawasaki disease and autoimmune disorders. Genetic variations in the ITPKC gene have been linked to an increased risk of Kawasaki disease a condition affecting blood vessels in children. In autoimmune diseases the altered regulation of calcium signaling pathways often involving proteins like calcineurin highlights the role of ITPKC in immune cell activation and inflammation. These associations emphasize the clinical importance of ITPKC in therapeutic research and potential treatments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the phosphorylation of 1D-myo-inositol 1,4,5-trisphosphate (InsP3) into 1D-myo-inositol 1,3,4,5-tetrakisphosphate and participates to the regulation of calcium homeostasis (PubMed : 11085927, PubMed : 12747803). Can phosphorylate inositol 2,4,5-triphosphate to inositol 2,4,5,6-tetraphosphate (By similarity).
See full target information ITPKC
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 7:12148 PubMed27401954

2016

Golgi membrane fission requires the CtBP1-S/BARS-induced activation of lysophosphatidic acid acyltransferase δ.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandro Pagliuso,Carmen Valente,Lucia Laura Giordano,Angela Filograna,Guiling Li,Diego Circolo,Gabriele Turacchio,Vincenzo Manuel Marzullo,Luigi Mandrich,Mikhail A Zhukovsky,Fabio Formiggini,Roman S Polishchuk,Daniela Corda,Alberto Luini
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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