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AB264282

Anti-ITPR3 antibody

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(1 Publication)

Rabbit Polyclonal ITPR3 antibody. Suitable for IHC-P, IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ITPR3 aa 600-700.

View Alternative Names

IP3 receptor isoform 3, IP3R-3, InsP3R3, Type 3 InsP3 receptor, ITPR3

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPR3 antibody (AB264282)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITPR3 antibody (AB264282)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human stomach carcinoma tissue labeling ITPR3 with ab264282 at 1/1,000 dilution. Detection : DAB.

Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Immunoprecipitation - Anti-ITPR3 antibody (AB264282)
  • IP

Supplier Data

Immunoprecipitation - Anti-ITPR3 antibody (AB264282)

ITPR3 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264282 at 3 μg per reaction. Western blot was performed from the immunoprecipitate using ab264282 at 1 μg/ml.

Lane 1 : ab264282 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

Exposure time : 3 mins.

All lanes:

Immunoprecipitation - Anti-ITPR3 antibody (ab264282)

Predicted band size: 304 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human ITPR3 aa 600-700. The exact immunogen used to generate this antibody is proprietary information.

Q14573

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The inositol 145-trisphosphate receptor type 3 (ITPR3) also known as IP3 receptor 3 is a calcium channel located on the membrane of the endoplasmic reticulum. It plays a critical role in the regulation of calcium ion concentration within cells. With an approximate mass of 313 kDa ITPR3 is expressed in various tissues with higher levels found in the brain pancreas and lung tissues. This protein facilitates the release of calcium from the endoplasmic reticulum into the cytoplasm triggering various cellular processes.
Biological function summary

The inositol 145-trisphosphate receptor type 3 regulates intracellular calcium signaling which is central for numerous physiological functions such as muscle contraction secretion metabolism and cell growth. ITPR3 functions as part of a channel complex that interacts with other proteins to mediate these calcium-dependent cellular responses. The protein's ability to bind with inositol 145-trisphosphate determines its channel activity influencing multiple downstream effects.

Pathways

Inositol 145-trisphosphate receptor type 3 integrates into the phosphoinositide signaling pathway and interacts with proteins such as STIM1 (stromal interaction molecule 1) and ORAI1 which are important for store-operated calcium entry (SOCE). The phosphoinositide pathway ensures efficient calcium mobilization and is essential for proper immune response and neurotransmission. ITPR3's function in these pathways highlights its fundamental role in managing calcium-dependent physiological processes.

Dysregulation of inositol 145-trisphosphate receptor type 3 can contribute to conditions like pancreatitis and neurodegenerative disorders. An imbalance in calcium homeostasis influenced by ITPR3 malfunction can lead to abnormal cell behavior and tissue damage. Additionally ITPR3 is connected to calmodulin in these diseases as both proteins play roles in calcium signaling pathways that when disrupted can exacerbate disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inositol 1,4,5-trisphosphate-gated calcium channel that, upon 1D-myo-inositol 1,4,5-trisphosphate binding, transports calcium from the endoplasmic reticulum lumen to cytoplasm, thus releasing the intracellular calcium and therefore participates in cellular calcium ion homeostasis (PubMed : 32949214, PubMed : 37898605, PubMed : 8081734, PubMed : 8288584, PubMed : 39560673). 1D-myo-inositol 1,4,5-trisphosphate binds to the ligand-free channel without altering its global conformation, yielding the low-energy resting state, then progresses through resting-to preactivated transitions to the higher energy preactivated state, which increases affinity for calcium, promoting binding of the low basal cytosolic calcium at the juxtamembrane domain (JD) site, favoring the transition through the ensemble of high-energy intermediate states along the trajectory to the fully-open activated state (PubMed : 30013099, PubMed : 35301323, PubMed : 37898605). Upon opening, releases calcium in the cytosol where it can bind to the low-affinity cytoplasmic domain (CD) site and stabilizes the inhibited state to terminate calcium release (PubMed : 30013099, PubMed : 35301323, PubMed : 37898605).
See full target information ITPR3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 40:65 PubMed33573671

2021

ITPR3 facilitates tumor growth, metastasis and stemness by inducing the NF-ĸB/CD44 pathway in urinary bladder carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Mengzhao Zhang,Lu Wang,Yangyang Yue,Lu Zhang,Tianjie Liu,Minxuan Jing,Xiao Liang,Minghai Ma,Shan Xu,Ke Wang,Xinyang Wang,Jinhai Fan
View all publications
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Product promise

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