AB264283

Anti-ITPR3 antibody

Name: Anti-ITPR3 antibody (ab264283) | Abcam
Brand: Abcam Limited
SKU: AB264283
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal ITPR3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ITPR3 aa 1750-1850.

View Alternative Names

IP3 receptor isoform 3, IP3R-3, InsP3R3, Type 3 InsP3 receptor, ITPR3

3 Images

Supplier Data

Immunoprecipitation - Anti-ITPR3 antibody (AB264283)

ITPR3 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264283 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab264283 at 1 μg/ml.

Lane 1 : ab264283 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

Exposure time : 10 secs.

All lanes:

Immunoprecipitation - Anti-ITPR3 antibody (ab264283)

Predicted band size: 304 kDa

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Lab

Western blot - Anti-ITPR3 antibody (AB264283)

Western blot : Anti-ITPR3 antibody ab264283 staining at 1/1000 dilution, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta.

A band was observed at 304 kDa in Wild-type HeLa UNBOILED cell lysates with no signal observed at this size in ITPR3 knockout HeLa UNBOILED cell line.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.

Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-ITPR3 antibody (ab264283) at 1/1000 dilution

Lane 1:

Wild-type HeLa UNBOILED at 20 µg

Lane 2:

ITPR3 knockout HeLa UNBOILED at 20 µg

Lane 3:

SH-SY5Y UNBOILED at 20 µg

Lane 4:

SK-N-FI UNBOILED at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 304 kDa

Observed band size: 304 kDa

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Supplier Data

Western blot - Anti-ITPR3 antibody (AB264283)

All lanes:

Western blot - Anti-ITPR3 antibody (ab264283) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 304 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ITPR3 aa 1750-1850. The exact immunogen used to generate this antibody is proprietary information.

Q14573

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The inositol 145-trisphosphate receptor type 3 (ITPR3) also known as IP3 receptor 3 is a calcium channel located on the membrane of the endoplasmic reticulum. It plays a critical role in the regulation of calcium ion concentration within cells. With an approximate mass of 313 kDa ITPR3 is expressed in various tissues with higher levels found in the brain pancreas and lung tissues. This protein facilitates the release of calcium from the endoplasmic reticulum into the cytoplasm triggering various cellular processes.

Biological function summary

The inositol 145-trisphosphate receptor type 3 regulates intracellular calcium signaling which is central for numerous physiological functions such as muscle contraction secretion metabolism and cell growth. ITPR3 functions as part of a channel complex that interacts with other proteins to mediate these calcium-dependent cellular responses. The protein's ability to bind with inositol 145-trisphosphate determines its channel activity influencing multiple downstream effects.

Pathways

Inositol 145-trisphosphate receptor type 3 integrates into the phosphoinositide signaling pathway and interacts with proteins such as STIM1 (stromal interaction molecule 1) and ORAI1 which are important for store-operated calcium entry (SOCE). The phosphoinositide pathway ensures efficient calcium mobilization and is essential for proper immune response and neurotransmission. ITPR3's function in these pathways highlights its fundamental role in managing calcium-dependent physiological processes.

Dysregulation of inositol 145-trisphosphate receptor type 3 can contribute to conditions like pancreatitis and neurodegenerative disorders. An imbalance in calcium homeostasis influenced by ITPR3 malfunction can lead to abnormal cell behavior and tissue damage. Additionally ITPR3 is connected to calmodulin in these diseases as both proteins play roles in calcium signaling pathways that when disrupted can exacerbate disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inositol 1,4,5-trisphosphate-gated calcium channel that, upon 1D-myo-inositol 1,4,5-trisphosphate binding, transports calcium from the endoplasmic reticulum lumen to cytoplasm, thus releasing the intracellular calcium and therefore participates in cellular calcium ion homeostasis (PubMed : 32949214, PubMed : 37898605, PubMed : 8081734, PubMed : 8288584). 1D-myo-inositol 1,4,5-trisphosphate binds to the ligand-free channel without altering its global conformation, yielding the low-energy resting state, then progresses through resting-to preactivated transitions to the higher energy preactivated state, which increases affinity for calcium, promoting binding of the low basal cytosolic calcium at the juxtamembrane domain (JD) site, favoring the transition through the ensemble of high-energy intermediate states along the trajectory to the fully-open activated state (PubMed : 30013099, PubMed : 35301323, PubMed : 37898605). Upon opening, releases calcium in the cytosol where it can bind to the low-affinity cytoplasmic domain (CD) site and stabilizes the inhibited state to terminate calcium release (PubMed : 30013099, PubMed : 35301323, PubMed : 37898605).

See full target information ITPR3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:25532 PubMed39462136

2024

Ca and Nε-lysine acetylation regulate the CALR-ATG9A interaction in the lumen of the endoplasmic reticulum.

Applications

Unspecified application

Species

Unspecified reactive species

Megan M Braun,Brendan K Sheehan,Samantha L Shapiro,Yun Ding,C Dustin Rubinstein,Brent P Lehman,Luigi Puglielli

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ITPR3 antibody

Immunoprecipitation - Anti-ITPR3 antibody (AB264283)

Western blot - Anti-ITPR3 antibody (AB264283)

Western blot - Anti-ITPR3 antibody (AB264283)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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