Anti-ITPR3 antibody [EPR25276-304]

• WB

Lab

Western blot - Anti-ITPR3 antibody [EPR25276-304] (AB325503)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

The identity of the bands lower than 130 kDa are unknown.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa).

All lanes:

Western blot - Anti-ITPR3 antibody [EPR25276-304] (ab325503) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control fresh whole cell lysate at 50 µg

Lane 2:

HeLa transfected with siRNA specifically targeting ITPR3 fresh whole cell lysate at 50 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 280 kDa,124 kDa

false

Exposure time: 15s

• WB

Lab

Western blot - Anti-ITPR3 antibody [EPR25276-304] (AB325503)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

To minimize protein degradation, cells (lanes 2-3) were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

The identity of the bands lower than 130 kDa are unknown.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa).

All lanes:

Western blot - Anti-ITPR3 antibody [EPR25276-304] (ab325503) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

DLD-1 (human colorectal adenocarcinoma epithelial cell) fresh whole cell lysate at 20 µg

Lane 3:

SH-SY5Y (human neuroblastoma epithelial cell) fresh whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 280 kDa,124 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-ITPR3 antibody [EPR25276-304] (AB325503)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human ITPR2 and ITPR1.

In Western blot, Anti-His tag® antibody (ab15149) (1 : 1000)(30-75KDa);Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-ITPR3 antibody [EPR25276-304] (ab325503) at 1/1000 dilution

Lane 1:

293T cells transfected with a human ITPR3 expression vector containing a his-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human ITPR2 expression vector containing a his-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human ITPR1 expression vector containing a his-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 74 kDa,30-75 kDa,36 kDa

false

Exposure time: 10s