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AB243509

Anti-IVD antibody

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(1 Publication)

Rabbit Polyclonal IVD antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human IVD aa 1-150.

View Alternative Names

IVD, Butyryl-CoA dehydrogenase

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IVD antibody (AB243509)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IVD antibody (AB243509)

Formalin-fixed, paraffin-embedded human thyroid gland tissue stained for IVD using ab243509 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IVD antibody (AB243509)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IVD antibody (AB243509)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for IVD using ab243509 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-IVD antibody (AB243509)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IVD antibody (AB243509)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for IVD (green) using ab243509 at 4 μg/ml in ICC/IF analysis.

Western blot - Anti-IVD antibody (AB243509)
  • WB

Supplier Data

Western blot - Anti-IVD antibody (AB243509)

All lanes:

Western blot - Anti-IVD antibody (ab243509) at 0.4 µg/mL

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) cell lysate

Predicted band size: 46 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human IVD aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P26440

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target IVD also known as isovaleryl-CoA dehydrogenase is an enzyme weighing approximately 49 kDa. It is a mitochondrial matrix enzyme where it is primarily expressed. IVD facilitates the third step of the leucine catabolic pathway by catalyzing the dehydrogenation of isovaleryl-CoA to 3-methylcrotonyl-CoA. This protein plays a critical role in the breakdown of leucine an essential amino acid contributing to energy production and metabolic processes.
Biological function summary

Isovaleryl-CoA dehydrogenase acts as an important enzyme in branched-chain amino acid metabolism. It is part of a complex enzyme system involved in leucine degradation. As such it contributes to maintaining energy homeostasis by converting leucine into acetoacetate and acetyl-CoA important components of energy production and lipid synthesis. IVD's functional activity supports metabolic processes and regulates amino acid levels in cells.

Pathways

Isovaleryl-CoA dehydrogenase participates in the leucine degradation pathway and the wider branched-chain amino acid degradation pathway. This pathway integrates with other metabolic pathways such as the tricarboxylic acid (TCA) cycle and the ketone body synthesis pathway. Related proteins in this context include enzymes such as 3-methylcrotonyl-CoA carboxylase and HMG-CoA reductase which also play roles in the metabolism of leucine and ketone body formation.

Disruptions in the activity of isovaleryl-CoA dehydrogenase can lead to isovaleric acidemia a metabolic disorder characterized by the inability to properly process leucine. This condition results in the accumulation of toxic metabolites leading to severe neurological deficits and metabolic crises. The protein is also linked to propionic acidemia where deficiencies in related enzymes like propionyl-CoA carboxylase or methylmalonyl-CoA mutase exacerbate symptoms highlighting the interconnected nature of metabolic pathways and the critical role of IVD.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the conversion of isovaleryl-CoA/3-methylbutanoyl-CoA to 3-methylbut-2-enoyl-CoA as an intermediate step in the leucine (Leu) catabolic pathway (PubMed : 7640268). To a lesser extent, is also able to catalyze the oxidation of other saturated short-chain acyl-CoA thioesters as pentanoyl-CoA, hexenoyl-CoA and butenoyl-CoA (PubMed : 7640268).
See full target information IVD
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadv8640 PubMed40512859

2025

Interaction between NF-κB and PLAC8 impairs autophagy providing a survival advantage to prostate cells transformed by cadmium.

Applications

Unspecified application

Species

Unspecified reactive species

Vaibhav Shukla,Ashish Tyagi,Balaji Chandrasekaran,Bhawna Tyagi,Balpreet Singh,Thulasidharan Nair Devanarayanan,Venkatesh Kolluru,Murali K Ankem,Chendil Damodaran
View all publications
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