Rabbit Polyclonal IVD antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IVD aa 1-150.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human IVD aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P26440
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Cow | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Catalyzes the conversion of isovaleryl-CoA/3-methylbutanoyl-CoA to 3-methylbut-2-enoyl-CoA as an intermediate step in the leucine (Leu) catabolic pathway (PubMed:7640268). To a lesser extent, is also able to catalyze the oxidation of other saturated short-chain acyl-CoA thioesters as pentanoyl-CoA, hexenoyl-CoA and butenoyl-CoA (PubMed:7640268).
Alternative names
IVD, Butyryl-CoA dehydrogenase
Recommended products
Rabbit Polyclonal IVD antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IVD aa 1-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human IVD aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link P26440
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The target IVD also known as isovaleryl-CoA dehydrogenase is an enzyme weighing approximately 49 kDa. It is a mitochondrial matrix enzyme where it is primarily expressed. IVD facilitates the third step of the leucine catabolic pathway by catalyzing the dehydrogenation of isovaleryl-CoA to 3-methylcrotonyl-CoA. This protein plays a critical role in the breakdown of leucine an essential amino acid contributing to energy production and metabolic processes.
- Biological function summary
Isovaleryl-CoA dehydrogenase acts as an important enzyme in branched-chain amino acid metabolism. It is part of a complex enzyme system involved in leucine degradation. As such it contributes to maintaining energy homeostasis by converting leucine into acetoacetate and acetyl-CoA important components of energy production and lipid synthesis. IVD's functional activity supports metabolic processes and regulates amino acid levels in cells.
- Pathways
Isovaleryl-CoA dehydrogenase participates in the leucine degradation pathway and the wider branched-chain amino acid degradation pathway. This pathway integrates with other metabolic pathways such as the tricarboxylic acid (TCA) cycle and the ketone body synthesis pathway. Related proteins in this context include enzymes such as 3-methylcrotonyl-CoA carboxylase and HMG-CoA reductase which also play roles in the metabolism of leucine and ketone body formation.
- Associated diseases and disorders
Disruptions in the activity of isovaleryl-CoA dehydrogenase can lead to isovaleric acidemia a metabolic disorder characterized by the inability to properly process leucine. This condition results in the accumulation of toxic metabolites leading to severe neurological deficits and metabolic crises. The protein is also linked to propionic acidemia where deficiencies in related enzymes like propionyl-CoA carboxylase or methylmalonyl-CoA mutase exacerbate symptoms highlighting the interconnected nature of metabolic pathways and the critical role of IVD.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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4 product images
Western blot - Anti-IVD antibody (ab243509)
All lanes: Western blot - Anti-IVD antibody (ab243509) at 0.4 µg/mL
Lane 1: RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2: U-251 MG (human brain glioma cell line) cell lysate
Predicted band size: 46 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IVD antibody (ab243509)
Formalin-fixed, paraffin-embedded human thyroid gland tissue stained for IVD using ab243509 at 1/200 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IVD antibody (ab243509)
Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for IVD using ab243509 at 1/200 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-IVD antibody (ab243509)
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for IVD (green) using ab243509 at 4 μg/ml in ICC/IF analysis.
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