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AB95994

Anti-IVD antibody

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(1 Publication)

Rabbit Polyclonal IVD antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human IVD aa 1-200.

View Alternative Names

IVD, Butyryl-CoA dehydrogenase

3 Images
Western blot - Anti-IVD antibody (AB95994)
  • WB

Unknown

Western blot - Anti-IVD antibody (AB95994)

10% SDS PAGE

All lanes:

Western blot - Anti-IVD antibody (ab95994) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 30 µg

Lane 2:

Jurkat whole cell lysate at 30 µg

Predicted band size: 46 kDa

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Western blot - Anti-IVD antibody (AB95994)
  • WB

Supplier Data

Western blot - Anti-IVD antibody (AB95994)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-IVD antibody (ab95994) at 1/1000 dilution

All lanes:

Mouse brain lysate at 50 µg

Predicted band size: 47 kDa

false

Western blot - Anti-IVD antibody (AB95994)
  • WB

Supplier Data

Western blot - Anti-IVD antibody (AB95994)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-IVD antibody (ab95994) at 1/1000 dilution

All lanes:

PC-12 whole cell lysate at 30 µg

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human, Mouse

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human IVD aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P26440

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target IVD also known as isovaleryl-CoA dehydrogenase is an enzyme weighing approximately 49 kDa. It is a mitochondrial matrix enzyme where it is primarily expressed. IVD facilitates the third step of the leucine catabolic pathway by catalyzing the dehydrogenation of isovaleryl-CoA to 3-methylcrotonyl-CoA. This protein plays a critical role in the breakdown of leucine an essential amino acid contributing to energy production and metabolic processes.
Biological function summary

Isovaleryl-CoA dehydrogenase acts as an important enzyme in branched-chain amino acid metabolism. It is part of a complex enzyme system involved in leucine degradation. As such it contributes to maintaining energy homeostasis by converting leucine into acetoacetate and acetyl-CoA important components of energy production and lipid synthesis. IVD's functional activity supports metabolic processes and regulates amino acid levels in cells.

Pathways

Isovaleryl-CoA dehydrogenase participates in the leucine degradation pathway and the wider branched-chain amino acid degradation pathway. This pathway integrates with other metabolic pathways such as the tricarboxylic acid (TCA) cycle and the ketone body synthesis pathway. Related proteins in this context include enzymes such as 3-methylcrotonyl-CoA carboxylase and HMG-CoA reductase which also play roles in the metabolism of leucine and ketone body formation.

Disruptions in the activity of isovaleryl-CoA dehydrogenase can lead to isovaleric acidemia a metabolic disorder characterized by the inability to properly process leucine. This condition results in the accumulation of toxic metabolites leading to severe neurological deficits and metabolic crises. The protein is also linked to propionic acidemia where deficiencies in related enzymes like propionyl-CoA carboxylase or methylmalonyl-CoA mutase exacerbate symptoms highlighting the interconnected nature of metabolic pathways and the critical role of IVD.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

A mitochondrial matrix enzyme that catalyzes the third step in leucine catabolism, where isovaleryl-CoA (3-methylbutanoyl-CoA) is metabolized to 3-methylbut-2-enoyl-CoA (PubMed : 7640268). To a lesser extent, it also participates in the first step in fatty acid beta-oxidation, in which it catalyzes the proR-proR stereospecific alpha,beta-dehydrogenation of other saturated short-chain acyl-CoA thioesters such as pentanoyl-CoA, hexanoyl-CoA and butanoyl-CoA, using the electron transfer flavoprotein (ETF) as their physiologic electron acceptor(PubMed : 7640268).
See full target information IVD
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular human reproduction 17:669-78 PubMed21593214

2011

Highly purified spermatozoal RNA obtained by a novel method indicates an unusual 28S/18S rRNA ratio and suggests impaired ribosome assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Heike Cappallo-Obermann,Wolfgang Schulze,Holger Jastrow,Vera Baukloh,Andrej-Nikolai Spiess
View all publications
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Product promise

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