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AB211626

Anti-IZUMO1 antibody [125]

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(1 Publication)

Rat Monoclonal IZUMO1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing Izumo1 protein.

View Alternative Names

Izumo sperm-egg fusion protein 1, Oocyte binding/fusion factor, Sperm-specific protein izumo, OBF, IZUMO1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-IZUMO1 antibody [125] (AB211626)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IZUMO1 antibody [125] (AB211626)

Immunofluorescent analysis of mouse spermatozoa labeling IZUMO1 using ab211626 at 4 μg/mL. An Alexa Fluor 488 conjugated anti-rat IgG was used as secondary antibody.

Western blot - Anti-IZUMO1 antibody [125] (AB211626)
  • WB

Supplier Data

Western blot - Anti-IZUMO1 antibody [125] (AB211626)

All lanes:

Western blot - Anti-IZUMO1 antibody [125] (ab211626) at 1/1000 dilution

Lane 1:

WT mouse sperm lysate at 10 µg

Lane 2:

IZUMO1 Knock out mouse sperm lysate at 10 µg

Predicted band size: 39 kDa,64 kDa

Observed band size: 65 kDa

false

Western blot - Anti-IZUMO1 antibody [125] (AB211626)
  • WB

Supplier Data

Western blot - Anti-IZUMO1 antibody [125] (AB211626)

All lanes:

Western blot - Anti-IZUMO1 antibody [125] (ab211626) at 1/100 dilution

Lane 1:

human testis lysate

Lane 2:

human sperm lysate

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab97057'>ab97057</a>) at 1/10000 dilution

Predicted band size: 39 kDa

false

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

125

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Cell preparation containing Izumo1 protein. The exact immunogen used to generate this antibody is proprietary information.

Q9D9J7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Ion exchange chromatography
Storage buffer
pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IZUMO1 also known as Immunoglobulin superfamily member 9 is a protein important to the process of fertilization. IZUMO1 has a molecular mass of approximately 37 kDa. It localizes specifically to the acrosomal region of sperm cells. This protein plays a mechanical role in sperm-egg fusion an essential step for successful fertilization allowing sperm cells to recognize and bind to the egg cell membrane. Expression of IZUMO1 is limited to mature sperm assuring its involvement exclusively during fertilization.
Biological function summary

The protein acts when spermatozoa interact with oocytes. IZUMO1 facilitates the binding through forming a complex with Juno the receptor on the egg surface. This interaction is necessary for the initial contact and fusion between the sperm and egg membranes. The IZUMO1-Juno pairing ensures proper docking and fusion enabling the combination of genetic materials from both gametes. The specificity of this binding prevents cross-species fertilization maintaining species integrity during reproduction.

Pathways

IZUMO1 has involvement in the fertilization pathway. It works directly in the sperm's fusion process an important part of the reproductive process. IZUMO1 interacts closely with other sperm proteins such as CD9 which play roles in adhesion and fusion events. These pathways incorporate IZUMO1 within the larger reproductive framework where it functions alongside these proteins to ensure successful gamete fusion and genetic transfer.

IZUMO1's functionality connects to issues of infertility. Defects or deficiencies in IZUMO1 expression or function can lead to male infertility due to ineffective sperm-egg fusion. Additionally fertility research identifies Juno as a complementary protein in this context as abnormalities in either protein may disrupt fertilization processes and contribute to reproductive challenges. Understanding IZUMO1 offers insight into diagnostic and therapeutic possibilities for treating infertility cases where sperm-egg membrane fusion is compromised.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1 : IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Acts a ligand for the human-specific oolemma epitope FCRL3/MAIA during fertilization (PubMed : 36070373). FCRL3/MAIA replaces IZUMO1R/JUNO as IZUMO1 receptor after sperm-egg adhesion, which permits species-specific gamete fusion (PubMed : 36070373). Plays a critical role in sperm-oolemma binding prior to plasma membrane fusion. Can mediate cell-cell fusion in cultured mammalian cells independently of its binding to IZUMO1R/JUNO (By similarity).
See full target information IZUMO1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 8:70142-70155 PubMed29050267

2017

Reversible regulation of ORC2 SUMOylation by PIAS4 and SENP2.

Applications

Unspecified application

Species

Unspecified reactive species

Ronghua Wang,Fangming Liu,Yongxu Zhao,Dan Wu,Lihan Chen,Edward T H Yeh,Chao Huang
View all publications
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Product promise

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