JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB211623

Anti-IZUMO1 antibody

Be the first to review this product! Submit a review

|

(6 Publications)

Rabbit Polyclonal IZUMO1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human IZUMO1 aa 200-300 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Izumo sperm-egg fusion protein 1, Oocyte binding/fusion factor, Sperm-specific protein izumo, OBF, IZUMO1

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-IZUMO1 antibody (AB211623)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IZUMO1 antibody (AB211623)

Immunofluorescent analysis of human sperm labeling IZUMO1 using ab211623 at 1/100 dilution. An Alexa Fluor594 conjugated anti-rabbit IgG at 1/1000 dilution was used as secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IZUMO1 antibody (AB211623)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IZUMO1 antibody (AB211623)

Immunohistochemical analysis of formalin fixed and paraffin embedded mouse testis labeling IZUMO1 using ab211623 at 1/100 dilution followed by secondary detection usin an Alexafluor 488 goat anti rabbit (H&L) IgG at 1/1000 dilution (green). Nuclear counterstain : DAPI (blue).

Western blot - Anti-IZUMO1 antibody (AB211623)
  • WB

Supplier Data

Western blot - Anti-IZUMO1 antibody (AB211623)

10-20% SDS Page gradient gel.

All lanes:

Western blot - Anti-IZUMO1 antibody (ab211623) at 1/1000 dilution

All lanes:

mouse sperm lysate at 10 µg

Predicted band size: 39 kDa

false

Western blot - Anti-IZUMO1 antibody (AB211623)
  • WB

Supplier Data

Western blot - Anti-IZUMO1 antibody (AB211623)

All lanes:

Western blot - Anti-IZUMO1 antibody (ab211623) at 1/1000 dilution

All lanes:

human sperm lysate at 20 µg

Predicted band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

This product was produced with the following immunogens:

The exact immunogen used to generate this antibody is proprietary information.

Synthetic Peptide within Human IZUMO1 aa 300 to C-terminus conjugated to Keyhole Limpet Haemocyanin. Database link Q8IYV9

The exact immunogen used to generate this antibody is proprietary information.

Synthetic Peptide within Human IZUMO1 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. Database link Q8IYV9

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/300", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
pH: 6 - 8.5 Preservative: 0.1% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IZUMO1 also known as Immunoglobulin superfamily member 9 is a protein important to the process of fertilization. IZUMO1 has a molecular mass of approximately 37 kDa. It localizes specifically to the acrosomal region of sperm cells. This protein plays a mechanical role in sperm-egg fusion an essential step for successful fertilization allowing sperm cells to recognize and bind to the egg cell membrane. Expression of IZUMO1 is limited to mature sperm assuring its involvement exclusively during fertilization.
Biological function summary

The protein acts when spermatozoa interact with oocytes. IZUMO1 facilitates the binding through forming a complex with Juno the receptor on the egg surface. This interaction is necessary for the initial contact and fusion between the sperm and egg membranes. The IZUMO1-Juno pairing ensures proper docking and fusion enabling the combination of genetic materials from both gametes. The specificity of this binding prevents cross-species fertilization maintaining species integrity during reproduction.

Pathways

IZUMO1 has involvement in the fertilization pathway. It works directly in the sperm's fusion process an important part of the reproductive process. IZUMO1 interacts closely with other sperm proteins such as CD9 which play roles in adhesion and fusion events. These pathways incorporate IZUMO1 within the larger reproductive framework where it functions alongside these proteins to ensure successful gamete fusion and genetic transfer.

IZUMO1's functionality connects to issues of infertility. Defects or deficiencies in IZUMO1 expression or function can lead to male infertility due to ineffective sperm-egg fusion. Additionally fertility research identifies Juno as a complementary protein in this context as abnormalities in either protein may disrupt fertilization processes and contribute to reproductive challenges. Understanding IZUMO1 offers insight into diagnostic and therapeutic possibilities for treating infertility cases where sperm-egg membrane fusion is compromised.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1 : IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Acts a ligand for the human-specific oolemma epitope FCRL3/MAIA during fertilization (PubMed : 36070373). FCRL3/MAIA replaces IZUMO1R/JUNO as IZUMO1 receptor after sperm-egg adhesion, which permits species-specific gamete fusion (PubMed : 36070373).
See full target information IZUMO1
style
left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 24:e70023 PubMed40248988

2025

Deciphering the Contribution of Circular RNAs to Age-Related Decline in Sertoli Cell Survivor.

Applications

Unspecified application

Species

Unspecified reactive species

Francesco Manfrevola,Nicola Mosca,Vincenza Grazia Mele,Teresa Chioccarelli,Guillaume Martinez,Charles Coutton,Monica Mattia,Mariaceleste Pezzullo,Silvia Fasano,Gilda Cobellis,Nicoletta Potenza,Rosanna Chianese

Human reproduction (Oxford, England) 39:1628-1644 PubMed38885964

2024

Variation of sperm quality and circular RNA content in men exposed to environmental contamination with heavy metals in 'Land of Fires', Italy.

Applications

Unspecified application

Species

Unspecified reactive species

Vincenza Grazia Mele,Teresa Chioccarelli,Nadia Diano,Donato Cappetta,Bruno Ferraro,Marialucia Telesca,Martina Moggio,Veronica Porreca,Antonella De Angelis,Liberato Berrino,Silvia Fasano,Gilda Cobellis,Rosanna Chianese,Francesco Manfrevola

Animals : an open access journal from MDPI 14: PubMed38612267

2024

Detection of Protein Biomarkers Relevant to Sperm Characteristics and Fertility in Semen in Three Wild Felidae: The Flat-Headed Cat (), Fishing Cat (), and Asiatic Golden Cat ().

Applications

Unspecified application

Species

Unspecified reactive species

Manita Wittayarat,Supalak Kiatsomboon,Navapol Kupthammasan,Wanlaya Tipkantha,Surasak Yimprasert,Ampika Thongphakdee,Saritvich Panyaboriban

International journal of biological sciences 19:2234-2255 PubMed37151878

2023

Cytochalasin D restores nuclear size acting on F-actin and IZUMO1 localization in low-quality spermatozoa.

Applications

Unspecified application

Species

Unspecified reactive species

Guillaume Martinez,Donato Cappetta,Marialucia Telesca,Konrad Urbanek,Giuseppe Castaldo,Magali Dhellemmes,Vincenza Grazia Mele,Teresa Chioccarelli,Veronica Porreca,Anne-Laure Barbotin,Angèle Boursier,Florian Guillou,Charles Coutton,Sophie Brouillet,Antonella De Angelis,Liberato Berrino,Riccardo Pierantoni,Gilda Cobellis,Rosanna Chianese,Francesco Manfrevola

International forum of allergy & rhinology 13:230-241 PubMed35950767

2022

Chronic interleukin-13 expression in mouse olfactory mucosa results in regional aneuronal epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Anirudh Saraswathula,Melissa M Liu,Heather Kulaga,Andrew P Lane

eLife 9: PubMed32484434

2020

TMEM95 is a sperm membrane protein essential for mammalian fertilization.

Applications

Unspecified application

Species

Unspecified reactive species

Ismael Lamas-Toranzo,Julieta G Hamze,Enrica Bianchi,Beatriz Fernández-Fuertes,Serafín Pérez-Cerezales,Ricardo Laguna-Barraza,Raúl Fernández-González,Pat Lonergan,Alfonso Gutiérrez-Adán,Gavin J Wright,María Jiménez-Movilla,Pablo Bermejo-Álvarez
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB211626

Anti-IZUMO1 antibody [125]

Immunocytochemistry/ Immunofluorescence - Anti-IZUMO1 antibody [125] (AB211626)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB252881

Anti-GFP antibody [3H9]

BOND RX™ Validated|Recombinant|Lab Essentials|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [3H9] (AB252881)

  • 0
  • 0
Proteins & Peptides

AB127522

Recombinant Human IZUMO1 protein

  • 0
  • 0
Primary Antibodies

AB37415

Rabbit IgG, polyclonal - Isotype Control

Flow Cytometry - Rabbit IgG, polyclonal - Isotype Control (AB37415)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com