Rabbit Polyclonal IZUMO1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human IZUMO1 aa 200-300 conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- International journal of biological sciences 19:2234-22552023Cytochalasin D restores nuclear size acting on F-actin and IZUMO1 localization in low-quality spermatozoa.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuillaume Martinez,Donato Cappetta,Marialucia Telesca,Konrad Urbanek,Giuseppe Castaldo,Magali Dhellemmes,Vincenza Grazia Mele,Teresa Chioccarelli,Veronica Porreca,Anne-Laure Barbotin,Angèle Boursier,Florian Guillou,Charles Coutton,Sophie Brouillet,Antonella De Angelis,Liberato Berrino,Riccardo Pierantoni,Gilda Cobellis,Rosanna Chianese,Francesco ManfrevolaPubMed 37151878
- eLife 9:2020TMEM95 is a sperm membrane protein essential for mammalian fertilization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIsmael Lamas-Toranzo et. al.PubMed 32484434
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6 - 8.5
Preservative: 0.1% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human IZUMO1 aa 200-300 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IYV9
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Mouse | Tested | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes Perform enzymatic antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/300.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Acts a ligand for the human-specific oolemma epitope FCRL3/MAIA during fertilization (PubMed:36070373). FCRL3/MAIA replaces IZUMO1R/JUNO as IZUMO1 receptor after sperm-egg adhesion, which permits species-specific gamete fusion (PubMed:36070373).
Alternative names
Izumo sperm-egg fusion protein 1, Oocyte binding/fusion factor, Sperm-specific protein izumo, OBF, IZUMO1
Recommended products
Rabbit Polyclonal IZUMO1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human IZUMO1 aa 200-300 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6 - 8.5
Preservative: 0.1% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
- Immunogens
- Synthetic Peptide within Human IZUMO1 aa 200-300 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IYV9
- Purity
- Whole antiserum
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IZUMO1 also known as Immunoglobulin superfamily member 9 is a protein important to the process of fertilization. IZUMO1 has a molecular mass of approximately 37 kDa. It localizes specifically to the acrosomal region of sperm cells. This protein plays a mechanical role in sperm-egg fusion an essential step for successful fertilization allowing sperm cells to recognize and bind to the egg cell membrane. Expression of IZUMO1 is limited to mature sperm assuring its involvement exclusively during fertilization.
- Biological function summary
The protein acts when spermatozoa interact with oocytes. IZUMO1 facilitates the binding through forming a complex with Juno the receptor on the egg surface. This interaction is necessary for the initial contact and fusion between the sperm and egg membranes. The IZUMO1-Juno pairing ensures proper docking and fusion enabling the combination of genetic materials from both gametes. The specificity of this binding prevents cross-species fertilization maintaining species integrity during reproduction.
- Pathways
IZUMO1 has involvement in the fertilization pathway. It works directly in the sperm's fusion process an important part of the reproductive process. IZUMO1 interacts closely with other sperm proteins such as CD9 which play roles in adhesion and fusion events. These pathways incorporate IZUMO1 within the larger reproductive framework where it functions alongside these proteins to ensure successful gamete fusion and genetic transfer.
- Associated diseases and disorders
IZUMO1's functionality connects to issues of infertility. Defects or deficiencies in IZUMO1 expression or function can lead to male infertility due to ineffective sperm-egg fusion. Additionally fertility research identifies Juno as a complementary protein in this context as abnormalities in either protein may disrupt fertilization processes and contribute to reproductive challenges. Understanding IZUMO1 offers insight into diagnostic and therapeutic possibilities for treating infertility cases where sperm-egg membrane fusion is compromised.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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4 product images
Western blot - Anti-IZUMO1 antibody (ab211623)
All lanes: Western blot - Anti-IZUMO1 antibody (ab211623) at 1/1000 dilution
All lanes: human sperm lysate at 20 µg
Predicted band size: 39 kDa
Western blot - Anti-IZUMO1 antibody (ab211623)
10-20% SDS Page gradient gel.
All lanes: Western blot - Anti-IZUMO1 antibody (ab211623) at 1/1000 dilution
All lanes: mouse sperm lysate at 10 µg
Predicted band size: 39 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IZUMO1 antibody (ab211623)
Immunohistochemical analysis of formalin fixed and paraffin embedded mouse testis labeling IZUMO1 using ab211623 at 1/100 dilution followed by secondary detection usin an Alexafluor 488 goat anti rabbit (H&L) IgG at 1/1000 dilution (green). Nuclear counterstain: DAPI (blue).
Immunocytochemistry/ Immunofluorescence - Anti-IZUMO1 antibody (ab211623)
Immunofluorescent analysis of human sperm labeling IZUMO1 using ab211623 at 1/100 dilution. An Alexa Fluor594 conjugated anti-rabbit IgG at 1/1000 dilution was used as secondary antibody.
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