Rabbit Polyclonal JAB1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human COPS5.
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Publications
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- The EMBO journal 38:2019Unanchored tri-NEDD8 inhibits PARP-1 to protect from oxidative stress-induced cell death.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatthew J Keuss et. al.PubMed 30804002
- PloS one 11:e01503642016Expression Atlas of the Deubiquitinating Enzymes in the Adult Mouse Retina, Their Evolutionary Diversification and Phenotypic Roles.Applications:IHCReactive species:Unspecified reactive speciesMariona Esquerdo et. al.PubMed 26934049
- Nature medicine 18:774-822012Adora2b-elicited Per2 stabilization promotes a HIF-dependent metabolic switch crucial for myocardial adaptation to ischemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTobias Eckle et. al.PubMed 22504483
- PloS one 6:e160712011Regulation of cullin RING E3 ubiquitin ligases by CAND1 in vivo.Applications:WBReactive species:HumanYee Shin Chua,Boon Kim Boh,Wanpen Ponyeam,Thilo HagenPubMed 21249194
- Biochemical and biophysical research communication 398:588-932010Biochemical and cellular effects of inhibiting Nedd8 conjugation.
- The Journal of biological chemistry 282:17032-402007Substrate-mediated regulation of cullin neddylation.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human COPS5. The exact immunogen used to generate this antibody is proprietary information. Database link Q92905
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Guinea pig | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
| Turkey | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000.00000 - 1/25000.00000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Orangutan, Xenopus tropicalis | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/250.00000 - 1/2000.00000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/250.00000 - 1/2000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Orangutan, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. Promotes the proteasomal degradation of BRSK2.
Alternative names
CSN5, JAB1, COPS5, COP9 signalosome complex subunit 5, SGN5, Signalosome subunit 5, Jun activation domain-binding protein 1
Recommended products
Rabbit Polyclonal JAB1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human COPS5.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human COPS5. The exact immunogen used to generate this antibody is proprietary information. Database link Q92905
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified using the peptide immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Centrifuge tube to remove product from lid.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
JAB1 also known as c-Jun activation domain-binding protein 1 or COP9 signalosome complex subunit 5 (COPS5) plays a mechanical role as part of the COP9 signalosome. It has a mass of around 37 kDa. JAB1 expresses in various tissues and connects with several proteins for controlling diverse cellular processes. The protein acts as a coactivator for transcription factors like c-Jun playing a part in cellular response to environmental changes.
- Biological function summary
The COP9 signalosome of which JAB1 is a part regulates protein degradation by modifying the activity of cullin-RING ubiquitin ligases. This complex plays an important part in processes like cell cycle progression and signal transduction. JAB1 influences cellular responses to stimuli by affecting the degradation of key regulatory proteins making it a component in maintaining protein homeostasis.
- Pathways
JAB1 integrates into signaling pathways like the TGF-β signaling pathway and the cell cycle regulation pathway. JAB1 interacts with other proteins including Smad7 in the TGF-β pathway facilitating the degradation of inhibitory Smads and therefore modulating the signal transduction. Through such interactions JAB1 contributes to the regulation of cellular proliferation and differentiation.
- Associated diseases and disorders
JAB1 relates to cancer progression and neurodegenerative diseases. Overexpression and misregulation can contribute to tumorigenesis as it influences the stability of proteins involved in cell growth. In the context of neurodegenerative diseases JAB1 interacts with proteins like p27kip1 impacting neuronal survival. Understanding these connections aids in studying their impact on disease development and progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
This image is courtesy of an anonymous customer reviewWestern blot - Anti-JAB1 antibody (ab12323)
All lanes: Western blot - Anti-JAB1 antibody (ab12323) at 1/2500 dilution
Lane 1: HEK293 whole cell lysate at 35 µg
Lane 2: HCT116 whole cell lysate at 35 µg
Lane 3: U2OS whole cell lysate at 35 µg
Lane 4: Mouse embryonic fibroblast whole cell lysate at 35 µg
SecondaryAll lanes: HRP-conjugated Goat anti-rabbit polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 35 kDa
Exposure time: 10s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAB1 antibody (ab12323)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse teratoma (right) tissues labelling JAB1 with ab12323 at 1/500 (2µg/ml). Detection: DAB.
Western blot - Anti-JAB1 antibody (ab12323)
Detection of Human CSN5 by Western Blot. Samples: Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN5 expression construct (T). Antibody: Affinity purified rabbit anti-CSN5 antibody BL1062 used at the indicated concentrations. Detection: Chemiluminescence with a 10 second exposure.
Detection of Human CSN5 by Western Blot. Samples: Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN5 expression construct (T). Antibody: Affinity purified rabbit anti-CSN5 antibody BL1062 used at the indicated concentrations. Detection: Chemiluminescence with a 10 second exposure.
All lanes: Western blot - Anti-JAB1 antibody (ab12323)
Predicted band size: 38 kDa
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