AB12323

Anti-JAB1 antibody

Name: Anti-JAB1 antibody (ab12323) | Abcam
Brand: Abcam Limited
SKU: AB12323
Price: 525 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(7 Publications)

Rabbit Polyclonal JAB1 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human COPS5.

View Alternative Names

CSN5, JAB1, COPS5, COP9 signalosome complex subunit 5, SGN5, Signalosome subunit 5, Jun activation domain-binding protein 1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAB1 antibody (AB12323)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAB1 antibody (AB12323)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse teratoma (right) tissues labelling JAB1 with ab12323 at 1/500 (2µg/ml). Detection : DAB.

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Western blot - Anti-JAB1 antibody (AB12323)

Detection of Human CSN5 by Western Blot. Samples : Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN5 expression construct (T). Antibody : Affinity purified rabbit anti-CSN5 antibody BL1062 used at the indicated concentrations. Detection : Chemiluminescence with a 10 second exposure.

Detection of Human CSN5 by Western Blot. Samples : Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN5 expression construct (T). Antibody : Affinity purified rabbit anti-CSN5 antibody BL1062 used at the indicated concentrations. Detection : Chemiluminescence with a 10 second exposure.

All lanes:

Western blot - Anti-JAB1 antibody (ab12323)

Predicted band size: 38 kDa

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AbReview40182****

Western blot - Anti-JAB1 antibody (AB12323)

All lanes:

Western blot - Anti-JAB1 antibody (ab12323) at 1/2500 dilution

Lane 1:

HEK293 whole cell lysate at 35 µg

Lane 2:

HCT116 whole cell lysate at 35 µg

Lane 3:

U2OS whole cell lysate at 35 µg

Lane 4:

Mouse embryonic fibroblast whole cell lysate at 35 µg

Secondary

All lanes:

HRP-conjugated Goat anti-rabbit polyclonal at 1/5000 dilution

Predicted band size: 38 kDa

Observed band size: 35 kDa

true

Exposure time: 10s

This image is courtesy of an anonymous Abreview

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human COPS5. The exact immunogen used to generate this antibody is proprietary information.

Q92905

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/25000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/2000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Turkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Centrifuge tube to remove product from lid.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified using the peptide immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

JAB1 also known as c-Jun activation domain-binding protein 1 or COP9 signalosome complex subunit 5 (COPS5) plays a mechanical role as part of the COP9 signalosome. It has a mass of around 37 kDa. JAB1 expresses in various tissues and connects with several proteins for controlling diverse cellular processes. The protein acts as a coactivator for transcription factors like c-Jun playing a part in cellular response to environmental changes.

Biological function summary

The COP9 signalosome of which JAB1 is a part regulates protein degradation by modifying the activity of cullin-RING ubiquitin ligases. This complex plays an important part in processes like cell cycle progression and signal transduction. JAB1 influences cellular responses to stimuli by affecting the degradation of key regulatory proteins making it a component in maintaining protein homeostasis.

Pathways

JAB1 integrates into signaling pathways like the TGF-β signaling pathway and the cell cycle regulation pathway. JAB1 interacts with other proteins including Smad7 in the TGF-β pathway facilitating the degradation of inhibitory Smads and therefore modulating the signal transduction. Through such interactions JAB1 contributes to the regulation of cellular proliferation and differentiation.

JAB1 relates to cancer progression and neurodegenerative diseases. Overexpression and misregulation can contribute to tumorigenesis as it influences the stability of proteins involved in cell growth. In the context of neurodegenerative diseases JAB1 interacts with proteins like p27kip1 impacting neuronal survival. Understanding these connections aids in studying their impact on disease development and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. Promotes the proteasomal degradation of BRSK2.

See full target information COPS5

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 26:1344-1366 PubMed39915298

2025

HSC70 coordinates COP9 signalosome and SCF ubiquitin ligase activity to enable a prompt stress response.

Applications

Unspecified application

Species

Unspecified reactive species

Shunsuke Nishimura,Hidetaka Kioka,Shan Ding,Hideyuki Hakui,Haruki Shinomiya,Kazuya Tanabe,Tatsuro Hitsumoto,Ken Matsuoka,Hisakazu Kato,Osamu Tsukamoto,Yoshihiro Asano,Seiji Takashima,Radoslav I Enchev,Yasushi Sakata

The EMBO journal 38: PubMed30804002

2019

Unanchored tri-NEDD8 inhibits PARP-1 to protect from oxidative stress-induced cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew J Keuss,Roland Hjerpe,Oliver Hsia,Robert Gourlay,Richard Burchmore,Matthias Trost,Thimo Kurz

PloS one 11:e0150364 PubMed26934049

2016

Expression Atlas of the Deubiquitinating Enzymes in the Adult Mouse Retina, Their Evolutionary Diversification and Phenotypic Roles.

Applications

IHC

Species

Unspecified reactive species

Mariona Esquerdo,Xavier Grau-Bové,Alejandro Garanto,Vasileios Toulis,Sílvia Garcia-Monclús,Erica Millo,Ma José López-Iniesta,Víctor Abad-Morales,Iñaki Ruiz-Trillo,Gemma Marfany

Nature medicine 18:774-82 PubMed22504483

2012

Adora2b-elicited Per2 stabilization promotes a HIF-dependent metabolic switch crucial for myocardial adaptation to ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Tobias Eckle,Katherine Hartmann,Stephanie Bonney,Susan Reithel,Michel Mittelbronn,Lori A Walker,Brian D Lowes,Jun Han,Christoph H Borchers,Peter M Buttrick,Douglas J Kominsky,Sean P Colgan,Holger K Eltzschig

PloS one 6:e16071 PubMed21249194

2011

Regulation of cullin RING E3 ubiquitin ligases by CAND1 in vivo.

Applications

Species

Human

Yee Shin Chua,Boon Kim Boh,Wanpen Ponyeam,Thilo Hagen

Biochemical and biophysical research communication 398:588-93 PubMed20603103

2010

Biochemical and cellular effects of inhibiting Nedd8 conjugation.

Applications

Species

Human

Yee Chin Leck,Yin Yin Choo,Chia Yee Tan,Peter G Smith,Thilo Hagen

The Journal of biological chemistry 282:17032-40 PubMed17439941

2007

Substrate-mediated regulation of cullin neddylation.

Applications

Species

Human

Eng-Hui Chew,Thilo Hagen

View all publications

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Product promise

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Anti-JAB1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAB1 antibody (AB12323)

Western blot - Anti-JAB1 antibody (AB12323)

Western blot - Anti-JAB1 antibody (AB12323)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise