AB124720

Anti-JAB1 antibody [EPR1350]

RabMAb
Recombinant
What is this?

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(10 Publications)

Rabbit Recombinant Monoclonal JAB1 antibody. Suitable for WB and reacts with Human, Rat, Mouse samples. Cited in 10 publications.

View Alternative Names

CSN5, JAB1, COPS5, COP9 signalosome complex subunit 5, SGN5, Signalosome subunit 5, Jun activation domain-binding protein 1

2 Images

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Western blot - Anti-JAB1 antibody [EPR1350] (AB124720)

All lanes:

Western blot - Anti-JAB1 antibody [EPR1350] (ab124720) at 1/1000 dilution

Lane 1:

NIH 3T3 cell lysate at 10 µg

Lane 2:

U87-MG cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Lane 4:

PC12 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 38 kDa

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OI-RD Scanning - Anti-JAB1 antibody [EPR1350] (AB124720)

OI-RD Scanning

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OI-RD Scanning - Anti-JAB1 antibody [EPR1350] (AB124720)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1350

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

JAB1 also known as c-Jun activation domain-binding protein 1 or COP9 signalosome complex subunit 5 (COPS5) plays a mechanical role as part of the COP9 signalosome. It has a mass of around 37 kDa. JAB1 expresses in various tissues and connects with several proteins for controlling diverse cellular processes. The protein acts as a coactivator for transcription factors like c-Jun playing a part in cellular response to environmental changes.

Biological function summary

The COP9 signalosome of which JAB1 is a part regulates protein degradation by modifying the activity of cullin-RING ubiquitin ligases. This complex plays an important part in processes like cell cycle progression and signal transduction. JAB1 influences cellular responses to stimuli by affecting the degradation of key regulatory proteins making it a component in maintaining protein homeostasis.

Pathways

JAB1 integrates into signaling pathways like the TGF-β signaling pathway and the cell cycle regulation pathway. JAB1 interacts with other proteins including Smad7 in the TGF-β pathway facilitating the degradation of inhibitory Smads and therefore modulating the signal transduction. Through such interactions JAB1 contributes to the regulation of cellular proliferation and differentiation.

JAB1 relates to cancer progression and neurodegenerative diseases. Overexpression and misregulation can contribute to tumorigenesis as it influences the stability of proteins involved in cell growth. In the context of neurodegenerative diseases JAB1 interacts with proteins like p27kip1 impacting neuronal survival. Understanding these connections aids in studying their impact on disease development and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. Promotes the proteasomal degradation of BRSK2.

See full target information COPS5

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastroenterology 28:2437-2456 PubMed35979263

2022

18β-glycyrrhetinic acid regulates mitochondrial ribosomal protein L35-associated apoptosis signaling pathways to inhibit proliferation of gastric carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Yuan,Yi Yang,Xia Li,Xin Zhou,Yu-Hua Du,Wen-Jing Liu,Lei Zhang,Lei Yu,Ting-Ting Ma,Jia-Xin Li,Yan Chen,Yi Nan

Genes 13: PubMed35627173

2022

Activation of Esterase D by FPD5 Inhibits Growth of A549 Lung Cancer Cells via JAB1/p53 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Yao,Yuejun Yang,Xinpeng Chen,Xiaoling Cui,Bangzhao Zhou,Baoxiang Zhao,Zhaomin Lin,Junying Miao

Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] 21:630-641 PubMed33707115

2021

Shikonin-mediated PD-L1 degradation suppresses immune evasion in pancreatic cancer by inhibiting NF-κB/STAT3 and NF-κB/CSN5 signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyan Ruan,Minhua Liang,Ling Shang,Manxiang Lai,Xiangliang Deng,Xinguo Su

Biomaterials 225:119539 PubMed31622821

2019

Nongenetic optical modulation of neural stem cell proliferation and neuronal/glial differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Meitian Wang,Zhiliang Xu,Qiao Liu,Wenjie Sun,Baichun Jiang,Kun Yang,Jiangxia Li,Yaoqin Gong,Qiji Liu,Duo Liu,Xi Li

eLife 6: PubMed28475037

2017

SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function.

Applications

Unspecified application

Species

Unspecified reactive species

Kate E Coleman,Miklós Békés,Jessica R Chapman,Sarah B Crist,Mathew Jk Jones,Beatrix M Ueberheide,Tony T Huang

The Journal of endocrinology 232:451-459 PubMed28053002

2017

Jab1 is a T2-dependent coactivator or a T3-dependent corepressor of TRB1-mediated gene regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriela Hernández-Puga,Arturo Mendoza,Alfonso León-Del-Río,Aurea Orozco

The Journal of biological chemistry 290:9299-309 PubMed25713139

2015

COPS5 protein overexpression increases amyloid plaque burden, decreases spinophilin-immunoreactive puncta, and exacerbates learning and memory deficits in the mouse brain.

Applications

Unspecified application

Species

Mouse

Ruizhi Wang,Hongjie Wang,Ivan Carrera,Shaohua Xu,Madepalli K Lakshmana

Journal of cellular physiology 230:1630-9 PubMed25545366

2014

miR-1470 mediates lapatinib induced p27 upregulation by targeting c-jun.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Nie,Wei Song,Wenwen Zhang,Yanru Wang,Aiyu Zhu,Jiaqing Shao,Xiaoxiang Guan

The Journal of biological chemistry 288:26668-77 PubMed23926111

2013

COPS5 (Jab1) protein increases β site processing of amyloid precursor protein and amyloid β peptide generation by stabilizing RanBP9 protein levels.

Applications

Unspecified application

Species

Unspecified reactive species

Hongjie Wang,Debleena Dey,Ivan Carrera,Dmitriy Minond,Elisabetta Bianchi,Shaohua Xu,Madepalli K Lakshmana

Biochimica et biophysica acta 1832:595-605 PubMed23357576

2013

X-linked intellectual disability gene CUL4B targets Jab1/CSN5 for degradation and regulates bone morphogenetic protein signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Fengjuan He,Defen Lu,Baichun Jiang,Yan Wang,Qiao Liu,Qiji Liu,Changshun Shao,Xi Li,Yaoqin Gong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-JAB1 antibody [EPR1350]

Western blot - Anti-JAB1 antibody [EPR1350] (AB124720)

OI-RD Scanning - Anti-JAB1 antibody [EPR1350] (AB124720)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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