Skip to main content

Rabbit Recombinant Monoclonal JAB1 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human, Rat samples.

Be the first to review this product! Submit a review

Images

OI-RD Scanning - Anti-JAB1 antibody [EPR1350] - BSA and Azide free (AB247980), expandable thumbnail
  • Western blot - Anti-JAB1 antibody [EPR1350] - BSA and Azide free (AB247980), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWB
Human
Not recommended
Tested
Mouse
Not recommended
Tested
Rat
Not recommended
Tested

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Target data

Function

Probable protease subunit of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of the SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. In the complex, it probably acts as the catalytic center that mediates the cleavage of Nedd8 from cullins. It however has no metalloprotease activity by itself and requires the other subunits of the CSN complex. Interacts directly with a large number of proteins that are regulated by the CSN complex, confirming a key role in the complex. Promotes the proteasomal degradation of BRSK2.

Alternative names

CSN5, JAB1, COPS5, COP9 signalosome complex subunit 5, SGN5, Signalosome subunit 5, Jun activation domain-binding protein 1

Recommended products

Rabbit Recombinant Monoclonal JAB1 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR1350
Purification technique
Affinity purification Protein A
Dissociation constant
6.45 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab247980 is the carrier-free version of Anti-JAB1 antibody [EPR1350] ab124720.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

JAB1 also known as c-Jun activation domain-binding protein 1 or COP9 signalosome complex subunit 5 (COPS5) plays a mechanical role as part of the COP9 signalosome. It has a mass of around 37 kDa. JAB1 expresses in various tissues and connects with several proteins for controlling diverse cellular processes. The protein acts as a coactivator for transcription factors like c-Jun playing a part in cellular response to environmental changes.

Biological function summary

The COP9 signalosome of which JAB1 is a part regulates protein degradation by modifying the activity of cullin-RING ubiquitin ligases. This complex plays an important part in processes like cell cycle progression and signal transduction. JAB1 influences cellular responses to stimuli by affecting the degradation of key regulatory proteins making it a component in maintaining protein homeostasis.

Pathways

JAB1 integrates into signaling pathways like the TGF-β signaling pathway and the cell cycle regulation pathway. JAB1 interacts with other proteins including Smad7 in the TGF-β pathway facilitating the degradation of inhibitory Smads and therefore modulating the signal transduction. Through such interactions JAB1 contributes to the regulation of cellular proliferation and differentiation.

Associated diseases and disorders

JAB1 relates to cancer progression and neurodegenerative diseases. Overexpression and misregulation can contribute to tumorigenesis as it influences the stability of proteins involved in cell growth. In the context of neurodegenerative diseases JAB1 interacts with proteins like p27kip1 impacting neuronal survival. Understanding these connections aids in studying their impact on disease development and progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • OI-RD Scanning - Anti-JAB1 antibody [EPR1350] - BSA and Azide free (ab247980), expandable thumbnail

    OI-RD Scanning - Anti-JAB1 antibody [EPR1350] - BSA and Azide free (ab247980)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Western blot - Anti-JAB1 antibody [EPR1350] - BSA and Azide free (ab247980), expandable thumbnail

    Western blot - Anti-JAB1 antibody [EPR1350] - BSA and Azide free (ab247980)

    This data was developed using Anti-JAB1 antibody [EPR1350] ab124720, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-JAB1 antibody [EPR1350] (Anti-JAB1 antibody [EPR1350] ab124720) at 1/1000 dilution

    Lane 1: NIH 3T3 cell lysate at 10 µg

    Lane 2: U87-MG cell lysate at 10 µg

    Lane 3: MCF7 cell lysate at 10 µg

    Lane 4: PC12 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 38 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com