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AB324802

Anti-Jagged 2/JAG2 antibody [EPR29026-90] - BSA and Azide free

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Rabbit Recombinant Monoclonal Jagged 2/JAG2 antibody. Carrier free. Suitable for Dot, WB and reacts with Recombinant fragment - Human, Human samples.

View Alternative Names

Protein jagged-2, Jagged2, hJ2, JAG2

5 Images
Western blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] - BSA and Azide free (AB324802)
  • WB

Supplier Data

Western blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] - BSA and Azide free (AB324802)

This data was developed using ab322107, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot ab322107 was shown to bind specifically to JAG2. Target of interest was observed at 180 kDa in wild-type Hela cell lysates (lane 1) with no signal observed at this size in JAR2 knockout cell line (lane 2) (lane 2 knockout cell line ab265966.

To minimize protein degradation cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

The identity of the bands between 30 kDa and 40 kDa are unknown.

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] (<a href='/en-us/products/primary-antibodies/jagged-2-jag2-antibody-epr29026-90-ab322107'>ab322107</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 60 µg

Lane 2:

Western blot - Human JAG2 (Jagged 2) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-jag2-jagged-2-knockout-hela-cell-line-ab265966'>ab265966</a>) at 60 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 180 kDa,36 kDa

false

Exposure time: 26s

Western blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] - BSA and Azide free (AB324802)
  • WB

Supplier Data

Western blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] - BSA and Azide free (AB324802)

This data was developed using ab322107, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Raji (PMID : 20133585).

To minimize protein degradation cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

The identity of the bands between 30 kDa and 40 kDa are unknown.

In Western blot Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] (<a href='/en-us/products/primary-antibodies/jagged-2-jag2-antibody-epr29026-90-ab322107'>ab322107</a>) at 1/1000 dilution

Lane 1:

SR (human pleural effusion lymphoblast) whole cell lysate at 20 µg

Lane 2:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 180 kDa,124 kDa

false

Exposure time: 26s

Western blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] - BSA and Azide free (AB324802)
  • WB

Supplier Data

Western blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] - BSA and Azide free (AB324802)

This data was developed using ab322107, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

To minimize protein degradation cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

The identity of the bands between 30 kDa and 40 kDa are unknown.

Exposure time : Lane 1 : 114 seconds Lane2 : 26 seconds

All lanes:

Western blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] (<a href='/en-us/products/primary-antibodies/jagged-2-jag2-antibody-epr29026-90-ab322107'>ab322107</a>) at 1/1000 dilution

Lane 1:

F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 60 µg

Lane 2:

HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 180 kDa

false

Dot Blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] - BSA and Azide free (AB324802)
  • Dot

Supplier Data

Dot Blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] - BSA and Azide free (AB324802)

This data was developed using ab322107, the same antibody clone in a different buffer formulation.

Dot blot analysis of Jagged 2/JAG2 using ab322107 at 1 : 1000 (0.529 ug/ml) followed by a Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) at 1 : 100000 dilution.

Lane1 : His-tagged human JAG2 fragment
Lane2 : His-tagged human JAG1

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human JAG1.

In Dot Blot Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Dot Blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] (<a href='/en-us/products/primary-antibodies/jagged-2-jag2-antibody-epr29026-90-ab322107'>ab322107</a>) at 1/1000 dilution

Lane 1:

His-tagged human JAG2 fragment

Lane 2:

His-tagged human JAG1 fragment

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

Western blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] - BSA and Azide free (AB324802)
  • WB

Supplier Data

Western blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] - BSA and Azide free (AB324802)

This data was developed using ab322107, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

To minimize protein degradation cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

The identity of the bands between 30 kDa and 40 kDa are unknown.

All lanes:

Western blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] (<a href='/en-us/products/primary-antibodies/jagged-2-jag2-antibody-epr29026-90-ab322107'>ab322107</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse testis tissue lysate at 20 µg

Lane 3:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 180 kDa

false

Exposure time: 114s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29026-90

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab324802 is the carrier-free version of ab322107.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Putative Notch ligand involved in the mediation of Notch signaling. Involved in limb development (By similarity).
See full target information JAG2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com