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AB233101

Anti-Jagged1 antibody

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(1 Publication)

Rabbit Polyclonal Jagged1 antibody. Suitable for WB, IHC-P and reacts with Pig, Mouse, Recombinant fragment - Human, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human JAG1 aa 800-1050.

View Alternative Names

CD339, JAGL1, JAG1, Protein jagged-1, Jagged1, hJ1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody (AB233101)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody (AB233101)

Paraffin-embedded human colon tissue stained for Jagged1 using ab233101 at 20 μg/ml in immunohistochemical analysis. AEC staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody (AB233101)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody (AB233101)

Paraffin-embedded human colon tissue stained for Jagged1 using ab233101 at 20 μg/ml in immunohistochemical analysis. AEC staining.

Western blot - Anti-Jagged1 antibody (AB233101)
  • WB

Unknown

Western blot - Anti-Jagged1 antibody (AB233101)

All lanes:

Western blot - Anti-Jagged1 antibody (ab233101) at 3 µg/mL

All lanes:

Recombinant human Jagged1 protein

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution

Predicted band size: 134 kDa,21 kDa

false

Western blot - Anti-Jagged1 antibody (AB233101)
  • WB

Unknown

Western blot - Anti-Jagged1 antibody (AB233101)

All lanes:

Western blot - Anti-Jagged1 antibody (ab233101) at 3 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 3:

Pig liver lysate

Lane 4:

Mouse liver lysate

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 134 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Pig

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human JAG1 aa 800-1050. The exact immunogen used to generate this antibody is proprietary information.

P78504

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab233101 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Jagged1 also known as JAG1 or Jagged 1 protein is a transmembrane protein involved in the Notch signaling pathway. It has a mass of approximately 150 kDa and is expressed in various tissues including the cardiovascular system liver and mammary glands. Not only does it play a role in cell-cell communication but it also engages in controlling cell differentiation proliferation and apoptosis. Additionally researchers studying its molecular nature frequently employ Jagged1 ELISA kits to measure its expression and activity levels.
Biological function summary

The Jagged1 protein acts as a ligand binding to Notch receptors forming a large complex with other critical proteins within the pathway. This interaction initiates the signal transduction necessary for various cellular processes reinforcing the influence Jagged1 has on development and tissue homeostasis. Its role as a ligand amplifies its importance and scientists often use Jagged1 ELISA and Jag1 ELISA assays to explore these biological interactions further.

Pathways

Research shows that Jagged1 is an integral component of the Notch signaling and cardiovascular development pathways. It binds to Notch1 and Notch2 receptors facilitating important steps in both pathways which are related to embryonic development and adult tissue maintenance. This Notch-Jagged1 interaction also connects to Delta-like ligands creating an intricate network of proteins and pathways critical for a wide spectrum of cellular functions.

Jagged1 exhibits significant connections to Alagille syndrome and some cancers. Mutations in Jagged1 or disrupted interactions with Notch receptors often lead to the developmental defects characteristic of Alagille syndrome where both Jagged1 and Notch2 play significant roles. Additionally aberrant activation of the Notch signaling pathway involving Jagged1 has links to tumorigenesis in certain cancers opining avenues for potential therapeutic interventions against these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ligand for multiple Notch receptors and involved in the mediation of Notch signaling (PubMed : 18660822, PubMed : 20437614). May be involved in cell-fate decisions during hematopoiesis (PubMed : 9462510). Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).
See full target information JAG1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 9:eadf2695 PubMed37058569

2023

A conditional knockout rat resource of mitochondrial protein-coding genes via a DdCBE-induced premature stop codon.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Tan,Xiaolong Qi,Weining Kong,Jiachuan Jin,Dan Lu,Xu Zhang,Yue Wang,Siting Wang,Wei Dong,Xudong Shi,Wei Chen,Jianying Wang,Keru Li,Yuan Xie,Lijuan Gao,Feifei Guan,Kai Gao,Chaojun Li,Cheng Wang,Zhibin Hu,Lianfeng Zhang,Xuejiang Guo,Bin Shen,Yuanwu Ma
View all publications
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Product promise

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