Rabbit Polyclonal Jagged1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 90 publications. Immunogen corresponding to Synthetic Peptide within Human JAG1 aa 100-150 conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- Communications biology 6:5382023Identification of acquired Notch3 dependency in metastatic Head and Neck Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria Kondratyev,Aleksandra Pesic,Troy Ketela,Natalie Stickle,Christine Beswick,Zvi Shalev,Stefano Marastoni,Soroush Samadian,Anna Dvorkin-Gheva,Azin Sayad,Mikhail Bashkurov,Pedro Boasquevisque,Alessandro Datti,Trevor J Pugh,Carl Virtanen,Jason Moffat,Reidar A Grénman,Marianne Koritzinsky,Bradly G WoutersPubMed 37202533
- Environmental toxicology 38:1628-16402023Di-(2-ethylhexyl) phthalate (DEHP) promoted hepatic lipid accumulation by activating Notch signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuezhu Zhang,Honghao Qian,Jia Wang,Ying Zhu,Xiaohan Miao,Xu Li,Jianli Yin,Ruxuan Zhang,Jiaming Ye,Chuanyi Huo,Weisen Zhao,Lin YePubMed 36988346
- International archives of allergy and immunology 184:122-1312022B Lymphocytes Balance Th1/Th2 to Participate in Fibrotic Hypersensitivity Pneumonia Induced by Pigeon Shedding.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Wu,Wei Ding,Yafang Li,Wenyi Wang,Zhichuang Lian,Xirennayi Muhataer,Yuan Zhou,Qifeng Li,Xiaohong YangPubMed 36323228
- Investigative ophthalmology & visual science 63:142022RO4929097, a Selective γ-Secretase Inhibitor, Inhibits Subretinal Fibrosis Via Suppressing Notch and ERK1/2 Signaling in Laser-Induced Mouse Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChaoyang Zhang,Shiyue Qin,Hai Xie,Qinghua Qiu,Haiyan Wang,Jingting Zhang,Dawei Luo,Jingfa ZhangPubMed 36155746
- Andrology 10:1644-16592022TGF-β3 induces lactate production in Sertoli cell through inhibiting the Notch pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Xu,Shuyi Jiang,Ying Hu,Qiang Zhang,Wenhui SuPubMed 36057850
- Cellular and molecular life sciences : CMLS 79:4462022APE1 controls DICER1 expression in NSCLC through miR-33a and miR-130b.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiulia Antoniali,Emiliano Dalla,Giovanna Mangiapane,Xiaolong Zhao,Xinming Jing,Yi Cheng,Veronica De Sanctis,Dilara Ayyildiz,Silvano Piazza,Mengxia Li,Gianluca TellPubMed 35876890
- Annals of translational medicine 10:2262022LncRNA promotes the malignant progression of melanoma by targeting .Applications:Unspecified applicationReactive species:Unspecified reactive speciesYijie Xie,Guangxiong ChenPubMed 35280401
- Frontiers in endocrinology 12:7899392022Adipocytes-Derived Extracellular Vesicle-miR-26b Promotes Apoptosis of Cumulus Cells and Induces Polycystic Ovary Syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuannan Zhou,Yuanyuan Gu,Fangyue Zhou,Hongdao Zhang,Menglei Zhang,Ganrong Zhang,Ligang Wu,Keqin Hua,Jingxin DingPubMed 35222263
- Cell death & disease 13:1602022HOXA-AS2 contributes to regulatory T cell proliferation and immune tolerance in glioma through the miR-302a/KDM2A/JAG1 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuanhong Zhong,Bei Tao,Xianglong Li,Wei Xiang,Lilei Peng,Tangming Peng,Ligang Chen,Xiangguo Xia,Jian You,Xiaobo YangPubMed 35181676
- Journal of inflammation research 15:635-6482022IL-17 is a Potential Therapeutic Target in a Rodent Model of Otitis Media with Effusion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNanfeng Zhang,Tingting Qian,Shan Sun,Wei Cao,Zhixian Wang,Danling Liu,Peifan Li,Jingfang Wu,Huawei Li,Jianming YangPubMed 35140496
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human JAG1 aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P78504
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/2000 | Notes 150 kDa in mouse liver lysate. |
Species Human | Dilution info 1/500 - 1/2000 | Notes 150 kDa in mouse liver lysate. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Ligand for multiple Notch receptors and involved in the mediation of Notch signaling (PubMed:18660822, PubMed:20437614). May be involved in cell-fate decisions during hematopoiesis (PubMed:9462510). Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).
Alternative names
CD339, JAGL1, JAG1, Protein jagged-1, Jagged1, hJ1
Recommended products
Rabbit Polyclonal Jagged1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 90 publications. Immunogen corresponding to Synthetic Peptide within Human JAG1 aa 100-150 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human JAG1 aa 100-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P78504
- Purification technique
- Affinity purification Protein A
- Specificity
A BLAST analysis was used to suggest cross reactivity with Jagged-1 protein from human, chimpanzee, rat and mouse based on 100% homology with the immunizing sequence. Partial reactivity is expected against dog (81%) and Xenopus laevis (85%) based on partial sequence homologies as indicated. Reactivity against homologues from other sources is not known.
- Concentration
- Purification notes
This protein A purified antibody is directed against human Jagged-1 protein. The product was purified from mono-specific antiserum by affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Jagged1 also known as JAG1 or Jagged 1 protein is a transmembrane protein involved in the Notch signaling pathway. It has a mass of approximately 150 kDa and is expressed in various tissues including the cardiovascular system liver and mammary glands. Not only does it play a role in cell-cell communication but it also engages in controlling cell differentiation proliferation and apoptosis. Additionally researchers studying its molecular nature frequently employ Jagged1 ELISA kits to measure its expression and activity levels.
- Biological function summary
The Jagged1 protein acts as a ligand binding to Notch receptors forming a large complex with other critical proteins within the pathway. This interaction initiates the signal transduction necessary for various cellular processes reinforcing the influence Jagged1 has on development and tissue homeostasis. Its role as a ligand amplifies its importance and scientists often use Jagged1 ELISA and Jag1 ELISA assays to explore these biological interactions further.
- Pathways
Research shows that Jagged1 is an integral component of the Notch signaling and cardiovascular development pathways. It binds to Notch1 and Notch2 receptors facilitating important steps in both pathways which are related to embryonic development and adult tissue maintenance. This Notch-Jagged1 interaction also connects to Delta-like ligands creating an intricate network of proteins and pathways critical for a wide spectrum of cellular functions.
- Associated diseases and disorders
Jagged1 exhibits significant connections to Alagille syndrome and some cancers. Mutations in Jagged1 or disrupted interactions with Notch receptors often lead to the developmental defects characteristic of Alagille syndrome where both Jagged1 and Notch2 play significant roles. Additionally aberrant activation of the Notch signaling pathway involving Jagged1 has links to tumorigenesis in certain cancers opining avenues for potential therapeutic interventions against these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
This image is courtesy of the Martin Kast LaboratoryImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody (ab7771)
Formalin-fixed paraffin embedded IHC of normal human cervial tissues (40x magnification) stained with ab7771 (1/100 dilution). The slides were counterstained with hematoxylin
This image is courtesy of Aihua Ma, Univdersity of CardiffImmunocytochemistry/ Immunofluorescence - Anti-Jagged1 antibody (ab7771)
Staining of Human corneal epithelial cells with anti-Jagged1 (ab7771, 1/500). The Jagged1 (green staining) is localised to the cytoplasm and cell nucleus and is consistent with reports in the literature. The nucleus is stained with Bis benzimide (1/500, blue staining).
This image is courtesy of the Martin Kast LaboratoryImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody (ab7771)
Formalin-fixed paraffin embedded IHC of human cervical cancer tissues (40x magnification) stained with ab7771 (1/100 dilution). The slides were counterstained with hematoxylin
Western blot - Anti-Jagged1 antibody (ab7771)
ab7771 Rabbit polyclonal to Jagged1 (1/500) with secondary Goat anti-rabbit IgG antibody Goat Anti-Rabbit IgG H&L (HRP) ab6721 (1/5000)
Exposure time: 1 minute. Expected molecular weight: 134 kDaLanes 1 to 3 and 5: 20μg of cell lysate per lane
Lane 4: 20 μl of 1x LDS loading bufferLane 1: Human Brain tissue lysate
Lane 2: Human Kidney tissue Lysate
Lane 3: Human liver tissue lysate
Lane 4: 1x LDS loading buffer
Lane 5: Mouse liver lysateAll lanes: Western blot - Anti-Jagged1 antibody (ab7771)
Predicted band size: 134 kDa
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