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Rabbit Recombinant Monoclonal Jagged1 antibody. Suitable for IP, ICC/IF, WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.


Images

Western blot - Anti-Jagged1 antibody [EPR26134-17] (AB300561), expandable thumbnail
  • Western blot - Anti-Jagged1 antibody [EPR26134-17] (AB300561), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Jagged1 antibody [EPR26134-17] (AB300561), expandable thumbnail
  • Immunoprecipitation - Anti-Jagged1 antibody [EPR26134-17] (AB300561), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)ICC/IFIHC-PWB
Human
Expected
Not recommended
Expected
Not recommended
Tested
Mouse
Tested
Not recommended
Tested
Not recommended
Tested
Rat
Expected
Not recommended
Expected
Not recommended
Tested

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
1/30
Notes

-

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Target data

Function

Ligand for multiple Notch receptors and involved in the mediation of Notch signaling (PubMed:18660822, PubMed:20437614). May be involved in cell-fate decisions during hematopoiesis (PubMed:9462510). Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).

Alternative names

CD339, JAGL1, JAG1, Protein jagged-1, Jagged1, hJ1

Recommended products

Rabbit Recombinant Monoclonal Jagged1 antibody. Suitable for IP, ICC/IF, WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26134-17
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Jagged1 also known as JAG1 or Jagged 1 protein is a transmembrane protein involved in the Notch signaling pathway. It has a mass of approximately 150 kDa and is expressed in various tissues including the cardiovascular system liver and mammary glands. Not only does it play a role in cell-cell communication but it also engages in controlling cell differentiation proliferation and apoptosis. Additionally researchers studying its molecular nature frequently employ Jagged1 ELISA kits to measure its expression and activity levels.

Biological function summary

The Jagged1 protein acts as a ligand binding to Notch receptors forming a large complex with other critical proteins within the pathway. This interaction initiates the signal transduction necessary for various cellular processes reinforcing the influence Jagged1 has on development and tissue homeostasis. Its role as a ligand amplifies its importance and scientists often use Jagged1 ELISA and Jag1 ELISA assays to explore these biological interactions further.

Pathways

Research shows that Jagged1 is an integral component of the Notch signaling and cardiovascular development pathways. It binds to Notch1 and Notch2 receptors facilitating important steps in both pathways which are related to embryonic development and adult tissue maintenance. This Notch-Jagged1 interaction also connects to Delta-like ligands creating an intricate network of proteins and pathways critical for a wide spectrum of cellular functions.

Associated diseases and disorders

Jagged1 exhibits significant connections to Alagille syndrome and some cancers. Mutations in Jagged1 or disrupted interactions with Notch receptors often lead to the developmental defects characteristic of Alagille syndrome where both Jagged1 and Notch2 play significant roles. Additionally aberrant activation of the Notch signaling pathway involving Jagged1 has links to tumorigenesis in certain cancers opining avenues for potential therapeutic interventions against these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Jagged1 antibody [EPR26134-17] (ab300561), expandable thumbnail

    Western blot - Anti-Jagged1 antibody [EPR26134-17] (ab300561)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    200-kDa full length and 23-kDa C-terminal JAG1 are observed. The molecular weights are consistent with what has been described in the literature (PMID: 30890522, 30890522).

    Lane 3 of this blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-Jagged1 antibody [EPR26134-17] (ab300561) at 1/1000 dilution

    Lane 1: Hepa1-6 (mouse hepatoma epithelial cell), whole cell lysate

    Lane 2: bEnd.3 (mouse brain endothelioma), whole cell lysate

    Lane 3: AR42J (rat pancreatic tumor epithelial cell), whole cell lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 200 kDa, 23 kDa

  • Western blot - Anti-Jagged1 antibody [EPR26134-17] (ab300561), expandable thumbnail

    Western blot - Anti-Jagged1 antibody [EPR26134-17] (ab300561)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative controls: Jurkat, MOLT-4 (PMID: 30231940)

    200-kDa full length and 23-kDa C-terminal JAG1 are observed. The molecular weights are consistent with what has been described in the literature (PMID: 30890522, 30890522).

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-Jagged1 antibody [EPR26134-17] (ab300561) at 1/1000 dilution

    Lane 1: HepG2 (human hepatocellar carcinoma epithelial cell), whole cell lysate 20

    Lane 2: HUVEC (human umbilical vein endothelial cell), whole cell lysate

    Lane 3: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate

    Lane 4: MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 23 kDa, 200 kDa

    Exposure time: 3min

  • Immunocytochemistry/ Immunofluorescence - Anti-Jagged1 antibody [EPR26134-17] (ab300561), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Jagged1 antibody [EPR26134-17] (ab300561)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell) cells labelling Jagged1 with ab300561 at 1/500 (1.04 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic and membranous staining in Hepa1-6 cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunoprecipitation - Anti-Jagged1 antibody [EPR26134-17] (ab300561), expandable thumbnail

    Immunoprecipitation - Anti-Jagged1 antibody [EPR26134-17] (ab300561)

    Jagged1 was immunoprecipitated from 0.35 mg Hepa1-6 (mouse hepatoma epithelial cell), whole cell lysate 10 ug with ab300561 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300561 at ID270 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: Hepa1-6 (mouse hepatoma epithelial cell), whole cell lysate 10 ug
    Lane 2: abAB300561 IP in Hepa1-6 whole cell lysate
    Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of abXXXXX in Hepa1-6 whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 7.75 seconds
    200-kDa full length and 23-kDa C-terminal JAG1 are observed. The molecular weights are consistent with what has been described in the literature (PMID: 30890522, 30890522).

    All lanes: Immunoprecipitation - Anti-Jagged1 antibody [EPR26134-17] (ab300561) at 1/1000 dilution

    Lanes 1 - 2: Hepa1-6 whole cell lysate

    Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 7.75s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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