AB109536

Anti-Jagged1 antibody [EPR4290]

Name: Anti-Jagged1 antibody [EPR4290] (ab109536) | Abcam
Brand: Abcam Limited
SKU: AB109536
Price: 345 USD
Availability: InStock
Rating: 5 (4 reviews)

RabMAb
Recombinant
KO Validated
What is this?

(4 Reviews)

(67 Publications)

Rabbit Recombinant Monoclonal Jagged1 antibody. Suitable for WB and reacts with Human samples. Cited in 67 publications.

View Alternative Names

CD339, JAGL1, JAG1, Protein jagged-1, Jagged1, hJ1

2 Images

Lab

Western blot - Anti-Jagged1 antibody [EPR4290] (AB109536)

Lanes 1 - 2 : Merged signal (red and green). Green - ab109536 observed at 200 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab109536 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in JAG1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and JAG1 knockout samples were subjected to SDS-PAGE. ab109536 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Jagged1 antibody [EPR4290] (ab109536) at 1/10000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

JAG1 knockout HAP1 whole cell lysate at 20 µg

Predicted band size: 134 kDa

Observed band size: 200 kDa

false

Lab

Western blot - Anti-Jagged1 antibody [EPR4290] (AB109536)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative controls : Jurkat and MOLT-4 whole cell lysate (PMID : 30231940)

200-kDa full length and 23-kDa C-terminal JAG1 are observed. The molecular weights are consistent with what has been described in the literature (PMID : 30890522, 30890522).

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-Jagged1 antibody [EPR4290] (ab109536) at 1/10000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate

Lane 2:

HUVEC (human umbilical vein endothelial cell), whole cell lysate

Lane 3:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate

Lane 4:

MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 134 kDa

Observed band size: 23 kDa,200 kDa

true

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4290

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Jagged1 also known as JAG1 or Jagged 1 protein is a transmembrane protein involved in the Notch signaling pathway. It has a mass of approximately 150 kDa and is expressed in various tissues including the cardiovascular system liver and mammary glands. Not only does it play a role in cell-cell communication but it also engages in controlling cell differentiation proliferation and apoptosis. Additionally researchers studying its molecular nature frequently employ Jagged1 ELISA kits to measure its expression and activity levels.

Biological function summary

The Jagged1 protein acts as a ligand binding to Notch receptors forming a large complex with other critical proteins within the pathway. This interaction initiates the signal transduction necessary for various cellular processes reinforcing the influence Jagged1 has on development and tissue homeostasis. Its role as a ligand amplifies its importance and scientists often use Jagged1 ELISA and Jag1 ELISA assays to explore these biological interactions further.

Pathways

Research shows that Jagged1 is an integral component of the Notch signaling and cardiovascular development pathways. It binds to Notch1 and Notch2 receptors facilitating important steps in both pathways which are related to embryonic development and adult tissue maintenance. This Notch-Jagged1 interaction also connects to Delta-like ligands creating an intricate network of proteins and pathways critical for a wide spectrum of cellular functions.

Jagged1 exhibits significant connections to Alagille syndrome and some cancers. Mutations in Jagged1 or disrupted interactions with Notch receptors often lead to the developmental defects characteristic of Alagille syndrome where both Jagged1 and Notch2 play significant roles. Additionally aberrant activation of the Notch signaling pathway involving Jagged1 has links to tumorigenesis in certain cancers opining avenues for potential therapeutic interventions against these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ligand for multiple Notch receptors and involved in the mediation of Notch signaling (PubMed : 18660822, PubMed : 20437614). May be involved in cell-fate decisions during hematopoiesis (PubMed : 9462510). Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).

See full target information JAG1

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Publications (67)

Recent publications for all applications. Explore the full list and refine your search

Regenerative therapy 30:136-143 PubMed40535723

2025

Tangeretin suppresses RANKL-induced osteoclastogenesis and alleviates postmenopausal osteoporosis by inhibiting Notch signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Tengfei Wu,Fang Wang,Changqing Ai,Li Li,Fan Wu

Scientific reports 15:16205 PubMed40346122

2025

SHP1 and its downstream p38/SP1/PI3K/YAP/Notch-1 signaling in trophoblast cells suppressed the progression of Preeclampsia via inhibiting proliferation of SMCs.

Applications

Unspecified application

Species

Unspecified reactive species

Yan An,Chenyuan Cao,Shaosong Sun,Hongli Wu,Jinzhi Zhang,Rui Li,Yakun Zhao

Regenerative therapy 25:290-301 PubMed38318480

2024

circ-Amotl1 in extracellular vesicles derived from ADSCs improves wound healing by upregulating SPARC translation.

Applications

Unspecified application

Species

Unspecified reactive species

Dazhou Wu,Shengyi Chen,Dongdong Huang,Zhipeng Huang,Na Zhen,Zhenxu Zhou,Jicai Chen

Experimental and therapeutic medicine 26:527 PubMed37869646

2023

Salsolinol improves angiotensin II‑induced myocardial fibrosis via inhibition of LSD1 through regulation of the STAT3/Notch‑1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xian Zhang,Ze Shao,Yuchao Ni,Feilong Chen,Xia Yu,Jiasheng Wen

Frontiers in neuroanatomy 17:1188886 PubMed37351521

2023

Mapping of Notch signaling in the developing organ of Corti in common marmosets.

Applications

Unspecified application

Species

Unspecified reactive species

Makoto Hosoya,Masato Fujioka,Hideyuki Okano,Hiroyuki Ozawa

Cell proliferation 56:e13514 PubMed37272232

2023

Insufficient GDF15 expression predisposes women to unexplained recurrent pregnancy loss by impairing extravillous trophoblast invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Chunzi Lyu,Tianxiang Ni,Yaqiu Guo,Tingting Zhou,Zi-Jiang Chen,Junhao Yan,Yan Li

Pharmaceuticals (Basel, Switzerland) 16: PubMed36678509

2022

Sanyin Formula Enhances the Therapeutic Efficacy of Paclitaxel in Triple-Negative Breast Cancer Metastases through the JAK/STAT3 Pathway in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyu Wu,Chenping Sun,Xianghui Han,Yiyi Ye,Yuenong Qin,Sheng Liu

Cellular and molecular gastroenterology and hepatology 15:393-424 PubMed36216310

2022

Single-cell Transcriptomics Reveals Early Molecular and Immune Alterations Underlying the Serrated Neoplasia Pathway Toward Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Jie Zhou,Xiao-Fan Lu,Huimin Chen,Xin-Yuan Wang,Wenxuan Cheng,Qing-Wei Zhang,Jin-Nan Chen,Xiao-Yi Wang,Jing-Zheng Jin,Fang-Rong Yan,Haoyan Chen,Xiao-Bo Li

Regenerative therapy 20:165-186 PubMed35620640

2022

Critical roles of FGF, RA, and WNT signalling in the development of the human otic placode and subsequent lineages in a dish.

Applications

Unspecified application

Species

Unspecified reactive species

Tsubasa Saeki,Sho Yoshimatsu,Mitsuru Ishikawa,Chung-Chau Hon,Ikuko Koya,Shinsuke Shibata,Makoto Hosoya,Chika Saegusa,Kaoru Ogawa,Jay W Shin,Masato Fujioka,Hideyuki Okano

Neural development 17:6 PubMed35524278

2022

Early development of the cochlea of the common marmoset, a non-human primate model.

Applications

Unspecified application

Species

Unspecified reactive species

Makoto Hosoya,Masato Fujioka,Junko Okahara,Sho Yoshimatsu,Hideyuki Okano,Hiroyuki Ozawa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Jagged1 antibody [EPR4290]

Western blot - Anti-Jagged1 antibody [EPR4290] (AB109536)

Western blot - Anti-Jagged1 antibody [EPR4290] (AB109536)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (67)

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