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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Polyclonal JAK1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Rat samples. Cited in 12 publications.
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Liquid
Polyclonal
WB | ICC/IF | IHC-P | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Predicted | Predicted | Predicted |
Rat | Tested | Expected | Expected |
Chimpanzee | Predicted | Predicted | Predicted |
Chinese hamster | Predicted | Predicted | Predicted |
Dog | Predicted | Predicted | Predicted |
Gorilla | Predicted | Predicted | Predicted |
Macaque monkey | Predicted | Predicted | Predicted |
Pig | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster | Dilution info - | Notes - |
Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway (PubMed:7615558). Kinase partner for the interleukin (IL)-2 receptor (PubMed:11909529) as well as interleukin (IL)-10 receptor (PubMed:12133952).
Tyrosine-protein kinase JAK1, Janus kinase 1, JAK-1, JAK1B, JAK1A, JAK1
Rabbit Polyclonal JAK1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Rat samples. Cited in 12 publications.
Tyrosine-protein kinase JAK1, Janus kinase 1, JAK-1, JAK1B, JAK1A, JAK1
IgG
Rabbit
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This enzyme participates significantly in the immune response and hematopoiesis by being part of the JAK-STAT signaling pathway complex. When activated JAK1 phosphorylates specific tyrosine residues on the receptor creating docking sites for STAT proteins which then get phosphorylated by JAK1. This phosphorylation allows STAT proteins to dimerize and translocate to the nucleus affecting gene expression involved in growth survival and differentiation processes.
JAK1 also known as Janus Kinase 1 plays an essential role in transmitting signals within cells. It is a tyrosine kinase enzyme with a molecular weight of approximately 130 kDa. JAK1 is expressed broadly in various tissues and is involved in cytokine receptor signaling. The kinase activity of JAK1 gets activated upon cytokine binding to its respective receptor which leads to phosphorylation events and downstream signaling cascades.
JAK1 is importantly engaged in the JAK-STAT pathway and the Interferon signaling pathway. Within these pathways JAK1 collaborates closely with other proteins like JAK2 TYK2 and STAT proteins. It acts as a mediator of the cellular response to a variety of cytokines and growth factors playing a role in the regulation of immune responses and cell proliferation.
Dysregulation of JAK1 associates with autoimmune diseases like rheumatoid arthritis and certain cancers. JAK1 inhibitors have been developed as therapeutic agents for these conditions targeting its kinase activity to modulate aberrant signaling. The interaction between JAK1 and other kinases such as JAK2 and transcription factors like STATs highlights its importance in disease mechanisms providing a target for drugs to manage inflammatory and malignant disorders.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
All lanes: Western blot - Anti-JAK1 antibody (AB125051) at 1 µg/mL
All lanes: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 133 kDa
Observed band size: 132 kDa, 43 kDa
Exposure time: 8min
IHC image of JAK1 staining in Human breast fibroadenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab125051, 10μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Gel type: MOPS
Blocking buffer: 1% milk block
All lanes: Western blot - Anti-JAK1 antibody (AB125051) at 1 µg/mL
Lane 1: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
Lane 2: HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 10 µg
All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 133 kDa
Observed band size: 140 kDa, 90 kDa, 60 kDa, 80 kDa
Exposure time: 4min
ab125051 staining JAK1 in HCT116 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab125051 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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