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Rabbit Polyclonal JAK1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Rat samples. Cited in 12 publications.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Rat
Tested
Expected
Expected
Chimpanzee
Predicted
Predicted
Predicted
Chinese hamster
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Gorilla
Predicted
Predicted
Predicted
Macaque monkey
Predicted
Predicted
Predicted
Pig
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1 µg/mL

Notes

-

Species

Rat

Dilution info

1 µg/mL

Notes

-

Predicted
Predicted

Species

Mouse, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

5 µg/mL

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

10 µg/mL

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster

Dilution info

-

Notes

-

Target data

Function

Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway (PubMed:7615558). Kinase partner for the interleukin (IL)-2 receptor (PubMed:11909529) as well as interleukin (IL)-10 receptor (PubMed:12133952).

Alternative names

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Rabbit Polyclonal JAK1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Rat samples. Cited in 12 publications.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

This enzyme participates significantly in the immune response and hematopoiesis by being part of the JAK-STAT signaling pathway complex. When activated JAK1 phosphorylates specific tyrosine residues on the receptor creating docking sites for STAT proteins which then get phosphorylated by JAK1. This phosphorylation allows STAT proteins to dimerize and translocate to the nucleus affecting gene expression involved in growth survival and differentiation processes.

Activity summary

JAK1 also known as Janus Kinase 1 plays an essential role in transmitting signals within cells. It is a tyrosine kinase enzyme with a molecular weight of approximately 130 kDa. JAK1 is expressed broadly in various tissues and is involved in cytokine receptor signaling. The kinase activity of JAK1 gets activated upon cytokine binding to its respective receptor which leads to phosphorylation events and downstream signaling cascades.

Pathways

JAK1 is importantly engaged in the JAK-STAT pathway and the Interferon signaling pathway. Within these pathways JAK1 collaborates closely with other proteins like JAK2 TYK2 and STAT proteins. It acts as a mediator of the cellular response to a variety of cytokines and growth factors playing a role in the regulation of immune responses and cell proliferation.

Associated diseases and disorders

Dysregulation of JAK1 associates with autoimmune diseases like rheumatoid arthritis and certain cancers. JAK1 inhibitors have been developed as therapeutic agents for these conditions targeting its kinase activity to modulate aberrant signaling. The interaction between JAK1 and other kinases such as JAK2 and transcription factors like STATs highlights its importance in disease mechanisms providing a target for drugs to manage inflammatory and malignant disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-JAK1 antibody (ab125051), expandable thumbnail

    Western blot - Anti-JAK1 antibody (ab125051)

    All lanes: Western blot - Anti-JAK1 antibody (AB125051) at 1 µg/mL

    All lanes: PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 133 kDa

    Observed band size: 132 kDa, 43 kDa

    Exposure time: 8min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 antibody (ab125051), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK1 antibody (ab125051)

    IHC image of JAK1 staining in Human breast fibroadenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab125051, 10μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-JAK1 antibody (ab125051), expandable thumbnail

    Western blot - Anti-JAK1 antibody (ab125051)

    Gel type: MOPS
    Blocking buffer: 1% milk block

    All lanes: Western blot - Anti-JAK1 antibody (AB125051) at 1 µg/mL

    Lane 1: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

    Lane 2: HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

    Predicted band size: 133 kDa

    Observed band size: 140 kDa, 90 kDa, 60 kDa, 80 kDa

    Exposure time: 4min

  • Immunocytochemistry/ Immunofluorescence - Anti-JAK1 antibody (ab125051), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-JAK1 antibody (ab125051)

    ab125051 staining JAK1 in HCT116 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab125051 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
    Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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