Rabbit Polyclonal JAK2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 45 publications. Immunogen corresponding to Synthetic Peptide within Human JAK2.
Images
Publications
Filter by
- Experimental and therapeutic medicine 25:2312023Expression of long non‑coding RNA NONHSAT227927.1 and its effect on the JAK2/STAT3 signaling pathway and inflammation in patients with ankylosing spondylitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiang Ding,Jian Liu,Yanqiu SunPubMed 37123211
- BMC cancer 23:1792023FANCD2 inhibits ferroptosis by regulating the JAK2/STAT3 pathway in osteosarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXujun Li,Jiangyi LiuPubMed 36814203
- Nan fang yi ke da xue xue bao = Journal of Southern Medical University 42:367-3742022[Triptolide inhibits inflammatory response and migration of fibroblast like synovial cells in rheumatoid arthritis through the circRNA 0003353/JAK2/STAT3 signaling pathway].Applications:Unspecified applicationReactive species:Unspecified reactive speciesJ Wang,J Liu,J Wen,X WangPubMed 35426800
- BMC complementary medicine and therapies 22:732022Chrysoeriol suppresses hyperproliferation of rheumatoid arthritis fibroblast-like synoviocytes and inhibits JAK2/STAT3 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia-Ying Wu,Ying-Jie Chen,Xiu-Qiong Fu,Jun-Kui Li,Ji-Yao Chou,Cheng-Le Yin,Jing-Xuan Bai,Ying Wu,Xiao-Qi Wang,Amy Sze-Man Li,Lut Yi Wong,Zhi-Ling YuPubMed 35296317
- Cell death discovery 8:1212022The possible involvement of circRNA DMNT1/p53/JAK/STAT in gestational diabetes mellitus and preeclampsia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongqin Bao,Chaohui Zhuang,Yan Jiao,Li YangPubMed 35296654
- Medical science monitor : international medical journal of experimental and clinical research 28:e9349142022Curcumol Attenuates Endometriosis by Inhibiting the JAK2/STAT3 Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Wang,Xiao-Bo Nie,Su-Jun Liu,Jiao Liu,Wen-Hui BianPubMed 35279667
- Diagnostic pathology 16:932021CircRNA NRIP1 promotes papillary thyroid carcinoma progression by sponging mir-195-5p and modulating the P38 MAPK and JAK/STAT pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuang Li,Lijuan Zhu,Lijun Fu,Mingli Han,Ya Li,Zhaozhong Meng,Xinguang QiuPubMed 34689819
- Clinical and experimental pharmacology & physiology 49:113-1212021Naringin promotes osteogenesis and ameliorates osteoporosis development by targeting JAK2/STAT3 signalling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWang Wang,Jie Mao,Yan Chen,Jing Zuo,Lin Chen,Yajing Li,Yingqian Gao,Qibin LuPubMed 34525226
- Allergologia et immunopathologia 49:72-772021Coniferyl aldehyde alleviates LPS-induced WI-38 cell apoptosis and inflammation injury via JAK2-STAT1 pathway in acute pneumonia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYichun He,Qin Li,Weijun Zhou,Yanhong Gu,Yu JiangPubMed 34476925
- Allergologia et immunopathologia 49:42-482021Laboratory screening test with inhalant and food allergens in atopic Brazilian children and adolescents: a performance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna Carolina Rozalem Reali,Felipe Faria Pierotti,Carolina Sanchez Aranda,Renata Rodrigues Cocco,Emanuel S Cavalcanti Sarinho,Flávio Sano,Arnaldo Porto Neto,Nelson A Rosário,Herberto J Chong Neto,Ekaterini Simões Goudouris,Lilian Sanches Moraes,Neusa Falbo Wandalsen,Antônio Carlos Pastorino,Jackeline Motta Franco,Maria Letícia Chavarria,Magnus P Borres,Dirceu SoléPubMed 34476921
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human JAK2. The exact immunogen used to generate this antibody is proprietary information. Database link O60674
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin receptor (MPL/TPOR); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins (PubMed:15690087, PubMed:7615558, PubMed:9657743, PubMed:15899890). Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins (PubMed:15690087, PubMed:9618263). Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain (PubMed:9657743). Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. Part of a signaling cascade that is activated by increased cellular retinol and that leads to the activation of STAT5 (STAT5A or STAT5B) (PubMed:21368206). In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation (PubMed:20098430). Plays a role in cell cycle by phosphorylating CDKN1B (PubMed:21423214). Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin (PubMed:19783980). Up-regulates the potassium voltage-gated channel activity of KCNA3 (PubMed:25644777).
Alternative names
Tyrosine-protein kinase JAK2, Janus kinase 2, JAK-2, JAK2
Recommended products
Rabbit Polyclonal JAK2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 45 publications. Immunogen corresponding to Synthetic Peptide within Human JAK2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human JAK2. The exact immunogen used to generate this antibody is proprietary information. Database link O60674
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Affinity purified using epitope-specific immunogen.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
JAK2 also known as Janus kinase 2 is a protein tyrosine kinase with a molecular weight of 125 kDa. It plays an essential mechanical role in the signaling pathways by acting as an intermediary between cell surface receptors and intracellular signaling molecules. The JAK2 protein binds to certain cytokine receptors facilitating signal transduction necessary for various cellular responses. JAK2 is expressed in many tissues including hematopoietic cells which are associated with the blood and immune systems.
- Biological function summary
JAK2 is important for transmitting signals that dictate cell growth survival and differentiation within the hematopoietic system. It operates bodily functions by forming complexes with specific phosphorylation sites on its associated receptors. Through this formation JAK2 influences the signaling cascade particularly by interacting with other signal transducers and activators where it phosphorylates and becomes activated. This action affects gene transcription directly correlated with cellular proliferation and differentiation processes.
- Pathways
The function of JAK2 integrates into the JAK-STAT signaling pathway which is an important pathway in the regulation of immune function growth and development. It works in conjunction with proteins such as STAT3 and STAT5 to transmit signals from cytokine receptors to the nucleus. This pathway critically impacts responses like inflammation and hematopoiesis aligning with its role in precursor proliferation within the bone marrow and various immune cells’ function.
- Associated diseases and disorders
JAK2 has significant implications in conditions like myeloproliferative neoplasms and polycythemia vera. Mutations in the JAK2 gene notably the JAK2 V617F mutation lead to uncontrolled cell division as they disrupt normal signaling mechanisms often resulting in blood cell disorders. In these contexts JAK2 interacts with proteins such as EpoR and MPL which play roles within these disease pathways. Understanding how JAK2 mutations contribute to disease progression offers pathways for targeted therapies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK2 antibody (ab39636)
ab39636 at a 1/50 dilution, staining JAK2 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue. Left image shows section without blocking peptide. Right image shows section with blocking peptide.
Western blot - Anti-JAK2 antibody (ab39636)
All lanes: Western blot - Anti-JAK2 antibody (ab39636) at 1/500 dilution
Lane 1: K562 cells at 15 µg
Lane 2: K562 cells at 15 µg with Blocked with immunising peptide
Predicted band size: 131 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK2 antibody (ab39636)
Formalin-fixed, paraffin-embedded dog lung tissue stained fo JAK2 using ab39636 at 1/400 dilution in immunohistochemical analysis. Primary antibosy was incubated for 30 minutes at 20°C.
Heat mediated antigen retrieval was performed using 1 mM EDTA buffer pH 8.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com