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Anti-JAK2 antibody [EPR108(2)] ab108596 is a rabbit monoclonal antibody that is used in JAK2 western blotting and immunofluorescence. Suitable for human, mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity confirmed with JAK2 knockout cell line validation
- Antibody clone EPR108(2) is cited in over 220 publications
New 20 ul size available


Images

Western blot - Anti-JAK2 antibody [EPR108(2)] (AB108596), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-JAK2 antibody [EPR108(2)] (AB108596), expandable thumbnail
  • Western blot - Anti-JAK2 antibody [EPR108(2)] (AB108596), expandable thumbnail
  • Western blot - Anti-JAK2 antibody [EPR108(2)] (AB108596), expandable thumbnail
  • Immunoprecipitation - Anti-JAK2 antibody [EPR108(2)] (AB108596), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IF
Human
Tested
Tested
Not recommended
Tested
Mouse
Expected
Tested
Not recommended
Expected
Rat
Expected
Tested
Not recommended
Expected

Tested
Tested

Species

Human

Dilution info

1/80

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/5000

Notes

For unpurified use at 1/1000.

Species

Rat

Dilution info

1/5000

Notes

For unpurified use at 1/1000.

Species

Human

Dilution info

1/5000

Notes

For unpurified use at 1/1000.

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/150

Notes

For unpurified use at 1/100 - 1/500.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins (PubMed:7615558). Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins (PubMed:9618263). Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. Part of a signaling cascade that is activated by increased cellular retinol and that leads to the activation of STAT5 (STAT5A or STAT5B) (PubMed:21368206). In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation (PubMed:20098430). Plays a role in cell cycle by phosphorylating CDKN1B (PubMed:21423214). Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin (PubMed:19783980).

Alternative names

Tyrosine-protein kinase JAK2, Janus kinase 2, JAK-2, JAK2

Recommended products

Anti-JAK2 antibody [EPR108(2)] ab108596 is a rabbit monoclonal antibody that is used in JAK2 western blotting and immunofluorescence. Suitable for human, mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity confirmed with JAK2 knockout cell line validation
- Antibody clone EPR108(2) is cited in over 220 publications
New 20 ul size available

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR108(2)

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

JAK2 also known as Janus kinase 2 is a protein tyrosine kinase with a molecular weight of 125 kDa. It plays an essential mechanical role in the signaling pathways by acting as an intermediary between cell surface receptors and intracellular signaling molecules. The JAK2 protein binds to certain cytokine receptors facilitating signal transduction necessary for various cellular responses. JAK2 is expressed in many tissues including hematopoietic cells which are associated with the blood and immune systems.

Biological function summary

JAK2 is important for transmitting signals that dictate cell growth survival and differentiation within the hematopoietic system. It operates bodily functions by forming complexes with specific phosphorylation sites on its associated receptors. Through this formation JAK2 influences the signaling cascade particularly by interacting with other signal transducers and activators where it phosphorylates and becomes activated. This action affects gene transcription directly correlated with cellular proliferation and differentiation processes.

Pathways

The function of JAK2 integrates into the JAK-STAT signaling pathway which is an important pathway in the regulation of immune function growth and development. It works in conjunction with proteins such as STAT3 and STAT5 to transmit signals from cytokine receptors to the nucleus. This pathway critically impacts responses like inflammation and hematopoiesis aligning with its role in precursor proliferation within the bone marrow and various immune cells’ function.

Associated diseases and disorders

JAK2 has significant implications in conditions like myeloproliferative neoplasms and polycythemia vera. Mutations in the JAK2 gene notably the JAK2 V617F mutation lead to uncontrolled cell division as they disrupt normal signaling mechanisms often resulting in blood cell disorders. In these contexts JAK2 interacts with proteins such as EpoR and MPL which play roles within these disease pathways. Understanding how JAK2 mutations contribute to disease progression offers pathways for targeted therapies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-JAK2 antibody [EPR108(2)] (ab108596), expandable thumbnail

    Western blot - Anti-JAK2 antibody [EPR108(2)] (ab108596)

    Lanes 1- 4: Merged signal (red and green). Green - ab108596 observed at 131 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab108596 was shown to react with JAK2 in wild-type A549 cells in western blot. Loss of signal was observed when knockout cell line Human JAK2 knockout A549 cell line ab267113 (knockout cell lysate Human JAK2 knockout A549 cell lysate ab256963) was used. Wild-type A549 and JAK2 knockout A549 cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab108596 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-JAK2 antibody [EPR108(2)] (ab108596) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: JAK2 knockout A549 cell lysate at 20 µg

    Lane 3: K562 cell lysate at 20 µg

    Lane 4: Daudi cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 131 kDa

    Observed band size: 131 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-JAK2 antibody [EPR108(2)] (ab108596), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-JAK2 antibody [EPR108(2)] (ab108596)

    Immunocytochemistry/ Immunofluorescence analysis of K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells labeling JAK2 with purified ab108596 at 1/150 dilution (8.5μg/ml). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, a goat anti-rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used as the counter stain at 1/200 (2.5 μg/ml). PBS instead of the primary antibody was the negative control. DAPI was used as a nuclear counterstain.

  • Western blot - Anti-JAK2 antibody [EPR108(2)] (ab108596), expandable thumbnail

    Western blot - Anti-JAK2 antibody [EPR108(2)] (ab108596)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-JAK2 antibody [EPR108(2)] (ab108596) at 1/5000 dilution

    Lane 1: TF-1 (Human bone marrow erythroleukemia cell line) whole cell lysate at 20 µg

    Lane 2: K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg

    Lane 3: C6 (Rat glial tumour cell line) whole cell lysate at 20 µg

    Lane 4: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 131 kDa

    Observed band size: 130 kDa

  • Western blot - Anti-JAK2 antibody [EPR108(2)] (ab108596), expandable thumbnail

    Western blot - Anti-JAK2 antibody [EPR108(2)] (ab108596)

    All lanes: Western blot - Anti-JAK2 antibody [EPR108(2)] (ab108596) at 1/1000 dilution

    Lane 1: TF-1 cell lysate at 10 µg

    Lane 2: K562 cell lysate at 10 µg

    Lane 3: THP-1 cell lysate at 10 µg

    Lane 4: Jurkat cell lysate at 10 µg

    Lane 5: NIH3T3 cell lysate at 10 µg

    Lane 6: IM-9 cell lysate at 10 µg

    Predicted band size: 131 kDa

    Observed band size: 130 kDa

  • Immunoprecipitation - Anti-JAK2 antibody [EPR108(2)] (ab108596), expandable thumbnail

    Immunoprecipitation - Anti-JAK2 antibody [EPR108(2)] (ab108596)

    ab108596 at 1/80 dilution (20 μg/mL) immunoprecipitating JAK2 in K562 (Human chronic myelogenous leukemia lymphoblast) cell lysate.

    Lane 1 (input): K562(Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10μg

    Lane 2 (+): K562 whole cell lysate, 350μg + ab108596, 2μg

    Lane 3 (-): K562 cell lysate, 350μg + rabbit IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730), 2μg

    For western blotting, ab108596 at 1/500 dilution (3.0 μg/mL) and VeriBlot for IP Detection Reagent (HRP) ab131366 VeriBlot for IP (HRP) was used at 1/1000.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-JAK2 antibody [EPR108(2)] (ab108596)

    Predicted band size: 131 kDa

    Observed band size: 130 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-JAK2 antibody [EPR108(2)] (ab108596), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-JAK2 antibody [EPR108(2)] (ab108596)

    Immunocytochemistry/Immunofluorescence analysis of Jurkat (Human T cell leukemia cells from peripheral blood) cells labelling JAK2 with unpurified ab108596 at 1/300 (7.0 μg/mL). Cells were fixed with 4% Paraformaldehydeand permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000, 2 μg/mL) was used as the secondary antibody. Nuclei were stained with DAPI (blue).

    Confocal image showing nuclear and cytoplasmic staining on Jurkat cell line.

  • Immunocytochemistry/ Immunofluorescence - Anti-JAK2 antibody [EPR108(2)] (ab108596), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-JAK2 antibody [EPR108(2)] (ab108596)

    Immunocytochemistry/Immunofluorescence analysis of Ramos (Human Burkitt's lymphoma) cells labelling JAK2 with unpurified 108596 at 1/300 (7.0 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000, 2 μg/mL) was used as the secondary antibody. Nuclei were stained with DAPI (blue).

    Confocal image showing nuclear and cytoplasmic staining on Ramos cell line.

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