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Anti-JAK2 (phospho Y1007 + Y1008) antibody [E132] (ab32101) is a rabbit monoclonal antibody that is used to detect JAK1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF, ELISA, Dot Blot. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 200 publications

-Over 200 publications


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101), expandable thumbnail
  • Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101), expandable thumbnail
  • Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101), expandable thumbnail
  • Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (AB32101), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PELISADotWBICC/IFFlow Cyt (Intra)
Human
Tested
Expected
Tested
Tested
Tested
Tested
Mouse
Expected
Expected
Expected
Tested
Expected
Expected
Rat
Expected
Expected
Expected
Tested
Expected
Expected
Synthetic peptide
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
-
Notes

-

Expected
Expected

Species
Human, Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

The samples may require stimulation (E.g., Jurkat cells treated with pervanadate for 5 min)

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

The samples may require stimulation (E.g., Jurkat cells treated with pervanadate for 5 min)

Species
Human
Dilution info
1/1000 - 1/10000
Notes

The samples may require stimulation (E.g., Jurkat cells treated with pervanadate for 5 min)

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/20
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin receptor (MPL/TPOR); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins (PubMed:15690087, PubMed:7615558, PubMed:9657743, PubMed:15899890). Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins (PubMed:15690087, PubMed:9618263). Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain (PubMed:9657743). Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. Part of a signaling cascade that is activated by increased cellular retinol and that leads to the activation of STAT5 (STAT5A or STAT5B) (PubMed:21368206). In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation (PubMed:20098430). Plays a role in cell cycle by phosphorylating CDKN1B (PubMed:21423214). Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin (PubMed:19783980). Up-regulates the potassium voltage-gated channel activity of KCNA3 (PubMed:25644777).

Additional Targets

JAK1 phospho Y1034 + Y1035

Alternative names

Tyrosine-protein kinase JAK2, Janus kinase 2, JAK-2, JAK2

Recommended products

Anti-JAK2 (phospho Y1007 + Y1008) antibody [E132] (ab32101) is a rabbit monoclonal antibody that is used to detect JAK1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF, ELISA, Dot Blot. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 200 publications

-Over 200 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
E132
Purification technique
Affinity purification Protein A
Specificity

This antibody is phospho-specific and only detects phosphorylated JAK2 on Tyrosine 1007 and 1008 (pY1007+Y1008) and JAK1 on Tyrosine 1034 and 1035 (pY1034+Y1035). According to our ELISA results, this antibody preferentially recognizes phospho Y1007. Stimulation may be required to allow detection of the phosphorylated protein. Please see images below for recommended treatment conditions and positive controls.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-JAK2 (phospho Y1007 + Y1008) antibody [E132] (ab32101) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA, Dot Blot in Human, Mouse, Rat samples.

What is the molecular weight of JAK1?


Anti-JAK2 (phospho Y1007 + Y1008) [E132] (ab32101) specifically detects a band for JAK1 (UniProt: O60674) at a molecular weight of 130kDa.

Trusted by the scientific community


Anti-JAK2 (phospho Y1007 + Y1008) [E132] (ab32101) was first used in a scientific publication in 2006 and has been cited over 200 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

JAK2 also known as Janus kinase 2 is a protein tyrosine kinase with a molecular weight of 125 kDa. It plays an essential mechanical role in the signaling pathways by acting as an intermediary between cell surface receptors and intracellular signaling molecules. The JAK2 protein binds to certain cytokine receptors facilitating signal transduction necessary for various cellular responses. JAK2 is expressed in many tissues including hematopoietic cells which are associated with the blood and immune systems.

Biological function summary

JAK2 is important for transmitting signals that dictate cell growth survival and differentiation within the hematopoietic system. It operates bodily functions by forming complexes with specific phosphorylation sites on its associated receptors. Through this formation JAK2 influences the signaling cascade particularly by interacting with other signal transducers and activators where it phosphorylates and becomes activated. This action affects gene transcription directly correlated with cellular proliferation and differentiation processes.

Pathways

The function of JAK2 integrates into the JAK-STAT signaling pathway which is an important pathway in the regulation of immune function growth and development. It works in conjunction with proteins such as STAT3 and STAT5 to transmit signals from cytokine receptors to the nucleus. This pathway critically impacts responses like inflammation and hematopoiesis aligning with its role in precursor proliferation within the bone marrow and various immune cells’ function.

Associated diseases and disorders

JAK2 has significant implications in conditions like myeloproliferative neoplasms and polycythemia vera. Mutations in the JAK2 gene notably the JAK2 V617F mutation lead to uncontrolled cell division as they disrupt normal signaling mechanisms often resulting in blood cell disorders. In these contexts JAK2 interacts with proteins such as EpoR and MPL which play roles within these disease pathways. Understanding how JAK2 mutations contribute to disease progression offers pathways for targeted therapies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101)

    JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody

    ab32101 showing positive staining in Human differentiated squamous cell carcinoma of the cervix tissue at 1/10000 dilution. Goat Anti-Rabbit IgG H&L (HRP) was used as secondary antibody. Antigen retreival was carried out by Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    Nuclear staining on human differentiated squamous cell carcinoma of the cervix without alkaline phosphatase treatment (image A). No staining on human differentiated squamous cell carcinoma of the cervix with alkaline phosphatase treatment (image B)

  • Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101), expandable thumbnail

    Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101)

    JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) Western blot staining using rabbit Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody

    The extra bands are undefined.

    All lanes: Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101) at 1/1000 dilution

    Lane 1: Hepa1-6 (Mouse hepatoma epithelial cell) whole cell lysate at 15 µg

    Lane 2: Hepa1-6 (Mouse hepatoma epithelial cell) treated with 100 μM pervanadate for 30 minutes whole cell lysate at 15 µg

    Lane 3: MEF (Mouse embryonic fibroblast (immortalized)) whole cell lysate at 15 µg

    Lane 4: MEF (Mouse embryonic fibroblast (immortalized)) treated with 100 μM pervanadate for 30 minutes whole cell lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 131 kDa

  • Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101), expandable thumbnail

    Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101)

    JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) Western blot staining using rabbit Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody

    All lanes: Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101) at 1/1000 dilution

    Lane 1: Mouse hippocampus lysate at 15 µg

    Lane 2: Mouse P240 hippocampus lysate at 15 µg

    Lane 3: Mouse P7 hippocampus lysate at 15 µg

    Lane 4: Rat hippocampus lysate at 15 µg

    Lane 5: Rat P7 hippocampus lysate at 15 µg

    Lane 6: Rat brain cortex lysate at 15 µg

    Lane 7: Human brain lysate at 15 µg

    Lane 8: Mouse brain lysate at 15 µg

    Lane 9: Rat brain lysate at 15 µg

    Lane 10: C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg

    Lane 11: C6 (Rat glial tumor glial cell) treated with 50mM pervanadate for 5 minutes whole cell lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 131 kDa

  • Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101), expandable thumbnail

    Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101)

    JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) Western blot staining using rabbit Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody

    Lanes 1 - 3: Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101) at 1/5000 dilution

    Lanes 1 - 3: Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] - BSA and Azide free (Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] - BSA and Azide free ab219728) at 1/5000 dilution

    Lane 1: Untreated Jurkat cells whole cell lysates at 10 µg

    Lane 2: Jurkat cells were treated with 50mM Pervanadate for 5 minutes whole cell lysates at 10 µg

    Lane 3: Jurkat cells were treated with 50mM Pervanadate for 5 minutes whole cell lysates. Then the membrane was incubated with Alkaline phosphatase. at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 131 kDa

    Observed band size: 120 kDa

    Exposure time: 5s

  • Immunocytochemistry/ Immunofluorescence - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101)

    JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody

    Immunocytochemistry/Immunofluorescence analysis of Jurkat +/- pervanadate (1mM, 30min) and Jurkat + pervanadate (1mM, 30min) + LP cells labelling JAK2 (phospho Y1007 + Y1008) with ab32101 at a dilution of 1/1000. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (goat anti-rabbit IgG Alexa Fluor® 488) (1/1000) was used as the secondary antibody. The cells were co-stained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at a 1/200 dilution. Nuclei counterstained with DAPI (blue).

  • Dot Blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101), expandable thumbnail

    Dot Blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101)

    JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) Dot Blot staining using rabbit Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody

    Dot blot analysis of human JAK2 (phospho Y1007 & Y1008) phospho peptide (Lane 1), JAK2 (phospho Y1007) phospho peptide (Lane 2), JAK2 (phospho Y1008) phospho peptide (Lane 3) and JAK2 non-phospho peptide (Lane 4) labelling JAK2 (phospho Y1007 & Y1008) with ab32101 at a dilution of 1/1000.

    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) was used as the secondary antibody at a dilution of 1/20,000.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101), expandable thumbnail

    Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101)

    JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) Western blot staining using rabbit Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody

    Exposure time:
    Left image: 1 second
    Right image: 5 minutes

    All lanes: Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101) at 1/1000 dilution

    Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 15 µg

    Lane 2: Jurkat (Human T cell leukemia T lymphocyte) treated with 50mM Pervanadate for 5 minutes whole cell lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 131 kDa

    Observed band size: 120 kDa, 60 kDa

  • Flow Cytometry (Intracellular) - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101)

    JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) Flow Cytometry (Intracellular) staining using rabbit Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody

    Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells starved of serum for 16 hours then treated with 1 mM Pervanadate for 30 minutes labeling JAK2 (phospho Y1007 + Y1008) with ab32101 at 1/20 dilution (10 ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr®488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control. Unstimulated Jurkat cells were used as a negative control (Green).

  • Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101)

    JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) Western blot staining using rabbit Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody

    All lanes: Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101) at 1/2000 dilution

    Lane 1: Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 0 hours. at 30 µg

    Lane 2: Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 15 minutes. at 30 µg

    Lane 3: Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 30 minutes. at 30 µg

    Lane 4: Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 1 hour. at 30 µg

    Lane 5: Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 2 hours. at 30 µg

    Lane 6: Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 4 hours. at 30 µg

    Lane 7: Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 6 hours. at 30 µg

    Lane 8: Rat uterine cell line - whole cell lysate. Treated with 1µg/mL Prolactin for 24 hours. at 30 µg

    Secondary

    All lanes: An HRP-conjugated donkey anti-rabbit polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 131 kDa

    Observed band size: 110 kDa, 55 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101)

    JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody

    ab32101 showing positive staining in Human differentiated squamous cell carcinoma of the cervix tissue at 1/10000 dilution. Goat Anti-Rabbit IgG H&L (HRP) was used as secondary antibody. Antigen retreival was carried out by Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    Nuclear staining on human differentiated squamous cell carcinoma of the cervix without alkaline phosphatase treatment (image A). No staining on human differentiated squamous cell carcinoma of the cervix with alkaline phosphatase treatment (image B)

  • ELISA - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101), expandable thumbnail

    ELISA - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101)

    Direct ELISA antigen dose-response curve using ab32101 at 0~1000 ng/mL. Antigen concentration of 10 ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (1/2500) was used as the secondary antibody.

    This antibody preferentially recognizes phospho Y1007. When the concentration of peptides is lower than 10 ng/mL, it cannot recognize phospho Y1008.

  • Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101), expandable thumbnail

    Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101)

    JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) Western blot staining using rabbit Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody

    All lanes: Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101)

    Lane 1: Mouse hippocampus lysate at 15 µg

    Lane 2: Mouse P240 hippocampus lysate at 15 µg

    Lane 3: Mouse P7 hippocampus lysate at 15 µg

    Lane 4: Rat hippocampus lysate at 15 µg

    Lane 5: Rat P7 hippocampus lysate at 15 µg

    Lane 6: Rat brain cortex lysate at 15 µg

    Lane 7: Human brain lysate at 15 µg

    Lane 8: Mouse brain lysate at 15 µg

    Lane 9: Rat brain lysate at 15 µg

    Lane 10: C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg

    Lane 11: C6 (Rat glial tumor glial cell) treated with 50mM pervanadate for 5 minutes whole cell lysate at 15 µg

    Predicted band size: 131 kDa

  • ELISA - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101), expandable thumbnail

    ELISA - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101)

    Direct ELISA antigen dose-response curve using ab32101 at 0~1000 ng/mL. Antigen concentration of 100 ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (1/2500) was used as the secondary antibody.

    This antibody preferentially recognizes phospho Y1007. When the concentration of peptides is higher than 100 ng/mL, it also recognizes phospho Y1008.

  • Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101), expandable thumbnail

    Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101)

    JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) Western blot staining using rabbit Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody

    Blocking and dilution buffer: 5% NFDM /TBST.

    All lanes: Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101) at 1/1000 dilution

    Lane 1: 293T cells transfected with a Human JAK1 expression vector containing a His tag whole cell lysate at 20 µg

    Lane 2: 293T cells transfected with a Human JAK1 expression vector containing a His tag were treated with 50uM pervanadate for 30 min whole cell lysate at 20 µg

    Lane 3: 293T cells transfected with a Human JAK1 (mutated Y1034A, mutated Y1035A) expression vector containing a His tag whole cell lysate at 20 µg

    Lane 4: 293T cells transfected with a Human JAK1 (mutated Y1034A, mutated Y1035A) expression vector containing a His tag were treated with 50uM pervanadate for 30 min whole cell lysate at 20 µg

    Lane 5: 293T cells transfected with a Human JAK2 expression vector containing a His tag whole cell lysate at 20 µg

    Lane 6: 293T cells transfected with a Human JAK2 expression vector containing a His tag were treated with 50uM pervanadate for 30 min whole cell lysate at 20 µg

    Lane 7: 293T cells transfected with a Human JAK2 (mutated Y1007A, mutated Y1008A) expression vector containing a His tag whole cell lysate at 20 µg

    Lane 8: 293T cells transfected with a Human JAK2 (mutated Y1007A, mutated Y1008A) expression vector containing a His tag were treated with 50uM pervanadate for 30 min whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 130 kDa

    Exposure time: 7s

  • Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101), expandable thumbnail

    Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101)

    JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) Western blot staining using rabbit Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody

    The extra bands are undefined.

    All lanes: Western blot - Anti-JAK2 (phospho Y1007 + Y1008) + JAK1 (phospho Y1034 + Y1035) antibody [E132] (ab32101) at 1/1000 dilution

    Lane 1: Hepa1-6 (Mouse hepatoma epithelial cell) whole cell lysate at 15 µg

    Lane 2: Hepa1-6 (Mouse hepatoma epithelial cell) treated with 100 μM pervanadate for 30 minutes whole cell lysate at 15 µg

    Lane 3: MEF (Mouse embryonic fibroblast (immortalized)) whole cell lysate at 15 µg

    Lane 4: MEF (Mouse embryonic fibroblast (immortalized)) treated with 100 μM pervanadate for 30 minutes whole cell lysate at 15 µg

    Predicted band size: 131 kDa

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