Rabbit Polyclonal JAK3 antibody. Suitable for IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human JAK3 aa 600-700 conjugated to Keyhole Limpet Haemocyanin.
Images
Publications
- Communications biology 4:4062021Endothelial Jak3 expression enhances pro-hematopoietic angiocrine function in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJosé Gabriel Barcia Durán,Tyler Lu,Sean Houghton,Fuqiang Geng,Ryan Schreiner,Jenny Xiang,Shahin Rafii,David Redmond,Raphaël LisPubMed 33767339
- Molecular medicine reports 19:2057-20642019Curculigoside exerts significant anti‑arthritic effects in vivo and in vitro via regulation of the JAK/STAT/NF‑κB signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShirui Tan et. al.PubMed 30664158
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human JAK3 aa 600-700 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P52333
Consider this alternative
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Mouse | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/500 | Notes When using a fluorescent probe the recommended dilution is 1/50-1/200. |
Species Human | Dilution info 1/100 - 1/500 | Notes When using a fluorescent probe the recommended dilution is 1/50-1/200. |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.
Alternative names
Tyrosine-protein kinase JAK3, Janus kinase 3, Leukocyte janus kinase, JAK-3, L-JAK, JAK3
Recommended products
Rabbit Polyclonal JAK3 antibody. Suitable for IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human JAK3 aa 600-700 conjugated to Keyhole Limpet Haemocyanin.
Alternative names
Tyrosine-protein kinase JAK3, Janus kinase 3, Leukocyte janus kinase, JAK-3, L-JAK, JAK3
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human JAK3 aa 600-700 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P52333
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- Activity summary
JAK3 also known as Janus kinase 3 is a protein with a molecular mass of approximately 125 kDa. It belongs to the Janus kinase family and plays an important role in the signal transduction pathways. JAK3 is expressed mainly in hematopoietic cells which include white blood cells and related precursor cells. Its primary function involves mediating signaling by associating with the common gamma chain used by cytokine receptors.
- Biological function summary
JAK3 is instrumental in the signaling mechanism of the immune system. It participates in the activation of various interleukins specifically those that signal through the common gamma chain receptor complex. JAK3's role is essential in the proper functioning and development of immune cells such as T cells and natural killer cells. Within this framework JAK3 pairs with cytokine receptors transmitting signals that regulate both proliferation and differentiation of these immune cells.
- Pathways
JAK3 integrates into JAK-STAT signaling pathways. This protein works alongside STAT proteins including STAT5 to facilitate the transcription of genes involved in cell survival and proliferation. It also interacts with JAK1 another member of the Janus kinase family to form functional complexes. These interactions are pivotal in maintaining normal immune responses and ensuring that signaling pathways are activated appropriately in response to extracellular cues.
- Associated diseases and disorders
Abnormal JAK3 function can lead to immune-related conditions. Severe combined immunodeficiency (SCID) is one such disorder arising from JAK3 mutations that impair immune cell function. Additionally JAK3 dysregulation links to malignancies such as T-cell acute lymphoblastic leukemia. Relating to these disorders JAK3 affects proteins like IL-7R and gamma chains which are important for cytokine signaling contributing to pathologies when malfunctioning.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK3 antibody (ab203611)
Immunohistochemical analysis of formalin-fixed paraffin embedded mouse lung tissue labeling Jak3 with ab203611 at 1/200 dilution. DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAK3 antibody (ab203611)
Immunohistochemical analysis of formalin-fixed paraffin embedded human lung carcinoma tissue labeling Jak3 with ab203611 at 1/200 dilution. DAB staining.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com