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AB278789

Anti-JAK3 (phospho Y980 + Y981) antibody [JAK3Y980981-E10]

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(2 Publications)

Rabbit Recombinant Monoclonal JAK3 phospho Y980 + Y981 antibody. Suitable for Flow Cyt, WB and reacts with Human, African green monkey samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human JAK3 phospho Y980 + Y981.

View Alternative Names

Tyrosine-protein kinase JAK3, Janus kinase 3, Leukocyte janus kinase, JAK-3, L-JAK, JAK3

3 Images
Flow Cytometry - Anti-JAK3 (phospho Y980 + Y981) antibody [JAK3Y980981-E10] (AB278789)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-JAK3 (phospho Y980 + Y981) antibody [JAK3Y980981-E10] (AB278789)

Flow cytometric analysis of Ramos secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using ab2797890 at 0.01 μg/mL.

Flow Cytometry - Anti-JAK3 (phospho Y980 + Y981) antibody [JAK3Y980981-E10] (AB278789)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-JAK3 (phospho Y980 + Y981) antibody [JAK3Y980981-E10] (AB278789)

Peptide blocking flow cytometric analysis of Ramos cells secondary antibody only negative control (light blue) or untreated (red) or treated with pervanate (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using ab278789 at 0.01 ug/mL.

Western blot - Anti-JAK3 (phospho Y980 + Y981) antibody [JAK3Y980981-E10] (AB278789)
  • WB

Supplier Data

Western blot - Anti-JAK3 (phospho Y980 + Y981) antibody [JAK3Y980981-E10] (AB278789)

Western blot analysis of COS7 cell extract, untreated or treated with pervanadate using ab278789 at 0.1 ug/mL.

All lanes:

Western blot - Anti-JAK3 (phospho Y980 + Y981) antibody [JAK3Y980981-E10] (ab278789) at 0.1 µg/mL

Lane 1:

African green monkey kidney fibroblast-like cell line cell extract (treated with pervanadate)

Lane 2:

African green monkey kidney fibroblast-like cell line cell extract (untreated)

Predicted band size: 125 kDa

false

  • 519 FITC

    FITC Anti-JAK3 (phospho Y980 + Y981) antibody [JAK3Y980981-E10]

  • 660 APC

    APC Anti-JAK3 (phospho Y980 + Y981) antibody [JAK3Y980981-E10]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

JAK3Y980981-E10

Isotype

IgG

Light chain type

kappa

Carrier free

No

Reacts with

Human, African green monkey

Applications

Flow Cyt, WB

applications

Immunogen

Synthetic Peptide within Human JAK3 phospho Y980 + Y981. The exact immunogen used to generate this antibody is proprietary information.

P52333

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "African green monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

JAK3 also known as Janus kinase 3 is a protein with a molecular mass of approximately 125 kDa. It belongs to the Janus kinase family and plays an important role in the signal transduction pathways. JAK3 is expressed mainly in hematopoietic cells which include white blood cells and related precursor cells. Its primary function involves mediating signaling by associating with the common gamma chain used by cytokine receptors.
Biological function summary

JAK3 is instrumental in the signaling mechanism of the immune system. It participates in the activation of various interleukins specifically those that signal through the common gamma chain receptor complex. JAK3's role is essential in the proper functioning and development of immune cells such as T cells and natural killer cells. Within this framework JAK3 pairs with cytokine receptors transmitting signals that regulate both proliferation and differentiation of these immune cells.

Pathways

JAK3 integrates into JAK-STAT signaling pathways. This protein works alongside STAT proteins including STAT5 to facilitate the transcription of genes involved in cell survival and proliferation. It also interacts with JAK1 another member of the Janus kinase family to form functional complexes. These interactions are pivotal in maintaining normal immune responses and ensuring that signaling pathways are activated appropriately in response to extracellular cues.

Abnormal JAK3 function can lead to immune-related conditions. Severe combined immunodeficiency (SCID) is one such disorder arising from JAK3 mutations that impair immune cell function. Additionally JAK3 dysregulation links to malignancies such as T-cell acute lymphoblastic leukemia. Relating to these disorders JAK3 affects proteins like IL-7R and gamma chains which are important for cytokine signaling contributing to pathologies when malfunctioning.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A and STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion.
See full target information JAK3 phospho Y980 + Y981
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Experimental & molecular medicine 54:2162-2174 PubMed36473935

2022

ASK1-ER stress pathway-mediated fibrotic-EV release contributes to the interaction of alveolar epithelial cells and lung fibroblasts to promote mechanical ventilation-induced pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ri Tang,Shuya Mei,Qiaoyi Xu,Jinhua Feng,Yang Zhou,Shunpeng Xing,Zhengyu He,Yuan Gao

Experimental and therapeutic medicine 22:1108 PubMed34504562

2021

Tofacitinib protects intestinal epithelial cells against oxygen-glucose deprivation/reoxygenation injury by inhibiting the JAK/STAT3 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Yang,Xiaoli Xie
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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