AB224327

Anti-JAM-C antibody

Name: Anti-JAM-C antibody (ab224327) | Abcam
Brand: Abcam Limited
SKU: AB224327

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(1 Publication)

Rabbit Polyclonal JAM-C antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human JAM3 aa 100-250.

View Alternative Names

UNQ859/PRO1868, JAM3, Junctional adhesion molecule C, JAM-C, JAM-2, Junctional adhesion molecule 3, JAM-3, JAMC

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAM-C antibody (AB224327)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAM-C antibody (AB224327)

Paraffin-embedded human fallopian tube stained for JAM-C using ab224327 at 1/20 dilution in immunohistochemical analysis. Shows strong positivity in cilia in glandular cells.

Immunocytochemistry/ Immunofluorescence - Anti-JAM-C antibody (AB224327)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-JAM-C antibody (AB224327)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for JAM-C (green) using ab224327 at 4 μg/ml in ICC/IF.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAM-C antibody (AB224327)

Paraffin-embedded human kidney stained for JAM-C using ab224327 at 1/20 dilution in immunohistochemical analysis. Shows moderate cytoplasmic positivity in cells in tubules.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAM-C antibody (AB224327)

Paraffin-embedded human testis stained for JAM-C using ab224327 at 1/20 dilution in immunohistochemical analysis. Shows strong membranous positivity in cells in seminiferous ducts.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAM-C antibody (AB224327)

Paraffin-embedded human skeletal muscle stained for JAM-C using ab224327 at 1/20 dilution in immunohistochemical analysis. Shows no cytoplasmic positivity in myocytes as expected.

Supplier Data

Western blot - Anti-JAM-C antibody (AB224327)

All lanes:

Western blot - Anti-JAM-C antibody (ab224327) at 1/100 dilution

All lanes:

BEWO cell lysate

Predicted band size: 35 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human JAM3 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q9BX67

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Junctional adhesion molecule-C (JAM-C) is a cell adhesion molecule belonging to the immunoglobulin superfamily also known as JAM3. It has a mass around 37 kDa. JAM-C is present on endothelial cells various immune cells and some epithelial cells. This protein functions mechanically in maintaining tight junctions that control paracellular permeability and are important in cell-to-cell interactions.

Biological function summary

Junctional adhesion molecule-C plays significant roles in leukocyte migration angiogenesis and the maintenance of vascular endothelial barrier function. JAM-C is part of a larger junctional adhesion molecule complex which includes JAM-A and JAM-B that collectively influence these processes. Its expression in tissues such as the heart and brain suggests specialized roles in physiological functions including cardiovascular regulation and CNS barriers.

Pathways

Junctional adhesion molecule-C is an integral component of the leukocyte transmigration and angiogenesis pathways. These pathways collaborate to allow immune cells to pass through vessel walls a fundamental process in immune surveillance and response. Additionally JAM-C interacts with integrins and other adhesion molecules facilitating cellular communication necessary for tissue repair and growth.

Dysregulation of junctional adhesion molecule-C expression associates with inflammatory diseases and cancer. Altered JAM-C levels have implications in autoimmune disorders where abnormal leukocyte extravasation occurs. Its connection to cancer involves interactions with proteins like VE-cadherin contributing to increased tumor angiogenesis and metastasis making it a potential target for therapeutic intervention in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Junctional adhesion protein that mediates heterotypic cell-cell interactions with its cognate receptor JAM2 to regulate different cellular processes (PubMed : 11590146, PubMed : 11823489). Plays a role in homing and mobilization of hematopoietic stem and progenitor cells within the bone marrow. At the surface of bone marrow stromal cells, it contributes to the retention of the hematopoietic stem and progenitor cells expressing JAM3 (PubMed : 11590146, PubMed : 24357068). Plays a central role in leukocytes extravasation by facilitating transmigration through the endothelium (By similarity). Plays a role in spermatogenesis where JAM2 and JAM3, which are respectively expressed by Sertoli and germ cells, mediate an interaction between both cell types and play an essential role in the anchorage of germ cells onto Sertoli cells and the assembly of cell polarity complexes during spermatid differentiation (By similarity). Also functions as a counter-receptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN) (PubMed : 12208882, PubMed : 15194813). Plays a role in angiogenesis (PubMed : 23255084). Plays a role in the regulation of cell migration (Probable). During myogenesis, it is involved in myocyte fusion (By similarity).. Soluble form of JAM-C. Promotes chemotaxis of vascular endothelial cells and stimulates angiogenesis.

See full target information JAM3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:111487 PubMed39759007

2025

miR-450b-5p promotes development of endometriosis by inhibiting the GABPA/HOXD10 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Huang,Yidan Wang,Ruiyun Li,Yongmei Liu,Yuan Yang

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-JAM-C antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAM-C antibody (AB224327)

Immunocytochemistry/ Immunofluorescence - Anti-JAM-C antibody (AB224327)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAM-C antibody (AB224327)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAM-C antibody (AB224327)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAM-C antibody (AB224327)

Western blot - Anti-JAM-C antibody (AB224327)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise