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AB318984

Anti-JAM-C antibody [EPR29224-576]

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Rabbit Recombinant Monoclonal JAM-C antibody. Suitable for Flow Cyt, ICC/IF, WB and reacts with Human samples.

View Alternative Names

UNQ859/PRO1868, JAM3, Junctional adhesion molecule C, JAM-C, JAM-2, Junctional adhesion molecule 3, JAM-3, JAMC

3 Images
Flow Cytometry - Anti-JAM-C antibody [EPR29224-576] (AB318984)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-JAM-C antibody [EPR29224-576] (AB318984)

Flow cytometric analysis of JEG-3 (human placenta epithelial cell) (Right) / HeLa (human cervical adenocarcinoma epithelial cell) (Left) cells labelling JAM-C with ab318984 at 1/500 dilution (0.1ug) (Magenta) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Negative control : HeLa (PMID : 19143587).

Immunocytochemistry/ Immunofluorescence - Anti-JAM-C antibody [EPR29224-576] (AB318984)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-JAM-C antibody [EPR29224-576] (AB318984)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized JEG-3 (human placenta epithelial cell) cells labelling JAM-C with ab318984 at 1/50 (10.02 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green).

Confocal image showing membrane staining in JEG-3 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Negative control : HeLa (PMID : 19143587).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

Western blot - Anti-JAM-C antibody [EPR29224-576] (AB318984)
  • WB

Supplier Data

Western blot - Anti-JAM-C antibody [EPR29224-576] (AB318984)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : HeLa, Caco-2 (PMID : 19143587), MCF7.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1 : 37 seconds, lanes 2-7 : 180 seconds

All lanes:

Western blot - Anti-JAM-C antibody [EPR29224-576] (ab318984) at 1/1000 dilution

Lane 1:

RD (human muscle multinucleated spindle-shaped cell) whole cell lysate at 50 µg

Lane 2:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 50 µg

Lane 3:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 50 µg

Lane 4:

JEG-3 (human placenta epithelial cell) whole cell lysate at 50 µg

Lane 5:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 6:

Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 7:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37 kDa,36 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29224-576

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Junctional adhesion molecule-C (JAM-C) is a cell adhesion molecule belonging to the immunoglobulin superfamily also known as JAM3. It has a mass around 37 kDa. JAM-C is present on endothelial cells various immune cells and some epithelial cells. This protein functions mechanically in maintaining tight junctions that control paracellular permeability and are important in cell-to-cell interactions.
Biological function summary

Junctional adhesion molecule-C plays significant roles in leukocyte migration angiogenesis and the maintenance of vascular endothelial barrier function. JAM-C is part of a larger junctional adhesion molecule complex which includes JAM-A and JAM-B that collectively influence these processes. Its expression in tissues such as the heart and brain suggests specialized roles in physiological functions including cardiovascular regulation and CNS barriers.

Pathways

Junctional adhesion molecule-C is an integral component of the leukocyte transmigration and angiogenesis pathways. These pathways collaborate to allow immune cells to pass through vessel walls a fundamental process in immune surveillance and response. Additionally JAM-C interacts with integrins and other adhesion molecules facilitating cellular communication necessary for tissue repair and growth.

Dysregulation of junctional adhesion molecule-C expression associates with inflammatory diseases and cancer. Altered JAM-C levels have implications in autoimmune disorders where abnormal leukocyte extravasation occurs. Its connection to cancer involves interactions with proteins like VE-cadherin contributing to increased tumor angiogenesis and metastasis making it a potential target for therapeutic intervention in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Junctional adhesion protein that mediates heterotypic cell-cell interactions with its cognate receptor JAM2 to regulate different cellular processes (PubMed : 11590146, PubMed : 11823489). Plays a role in homing and mobilization of hematopoietic stem and progenitor cells within the bone marrow. At the surface of bone marrow stromal cells, it contributes to the retention of the hematopoietic stem and progenitor cells expressing JAM3 (PubMed : 11590146, PubMed : 24357068). Plays a central role in leukocytes extravasation by facilitating transmigration through the endothelium (By similarity). Plays a role in spermatogenesis where JAM2 and JAM3, which are respectively expressed by Sertoli and germ cells, mediate an interaction between both cell types and play an essential role in the anchorage of germ cells onto Sertoli cells and the assembly of cell polarity complexes during spermatid differentiation (By similarity). Also functions as a counter-receptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN) (PubMed : 12208882, PubMed : 15194813). Plays a role in angiogenesis (PubMed : 23255084). Plays a role in the regulation of cell migration (Probable). During myogenesis, it is involved in myocyte fusion (By similarity).. Soluble form of JAM-C. Promotes chemotaxis of vascular endothelial cells and stimulates angiogenesis.
See full target information JAM3
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Product promise

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