JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB232632

Anti-JAML antibody [EPR15289] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal JAML antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

AMICA1, UNQ722/PRO1387, JAML, Junctional adhesion molecule-like, Adhesion molecule interacting with CXADR antigen 1, Dendritic cell-specific protein CREA7-1

2 Images
Immunoprecipitation - Anti-JAML antibody [EPR15289] - BSA and Azide free (AB232632)
  • IP

Supplier Data

Immunoprecipitation - Anti-JAML antibody [EPR15289] - BSA and Azide free (AB232632)

Western blot analysis of JAML in 293 cell lysate immunoprecipitated using ab183714 at 1/50 dilution (Lane 1). Lane 2 : TBS instead of 293 lysate.

Secondary antibody : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183714).

All lanes:

Immunoprecipitation - Anti-JAML antibody [EPR15289] (<a href='/en-us/products/primary-antibodies/jaml-antibody-epr15289-ab183714'>ab183714</a>)

Predicted band size: 44 kDa

false

Western blot - Anti-JAML antibody [EPR15289] - BSA and Azide free (AB232632)
  • WB

Supplier Data

Western blot - Anti-JAML antibody [EPR15289] - BSA and Azide free (AB232632)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183714).

All lanes:

Western blot - Anti-JAML antibody [EPR15289] (<a href='/en-us/products/primary-antibodies/jaml-antibody-epr15289-ab183714'>ab183714</a>) at 1/5000 dilution

Lane 1:

C6 (rat glioma cell line) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 44 kDa

Observed band size: 60 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15289

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Assay Kits

AB267597

Human AMICA ELISA Kit (JAML)

Sandwich ELISA - Human AMICA ELISA Kit (JAML) (AB267597)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB68153

Anti-STAT3 antibody [EPR787Y]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Western blot - Anti-STAT3 antibody [EPR787Y] (AB68153)

  • 4
  • 10
Primary Antibodies

AB76299

Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunoprecipitation - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (AB76299)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

ab232632 is the carrier-free version of ab183714.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein JAML also referred to as Junctional Adhesion Molecule-Like is an important transmembrane protein with a molecular mass of approximately 36 kDa. It belongs to the immunoglobulin superfamily and functions in the immune response. JAML is widely expressed in epithelial cells and various immune cells particularly in neutrophils and some subsets of T cells. On a cellular level JAML is involved in cell-cell adhesion processes functioning at interfaces where immune cells interact with epithelial layers.
Biological function summary

Beyond serving as a simple structural component JAML establishes interactions critical for immune surveillance and cell movement. It pairs with other immune receptors to promote the transmigration of leukocytes across endothelial and epithelial barriers. This interaction is notably important since JAML mediates adhesion through its engagement with the Coxsackievirus and Adenovirus Receptor (CAR) forming dynamic complexes that facilitate immune cell trafficking.

Pathways

Many cellular processes engage JAML in key signaling pathways responsible for immune responses. The protein takes part in the integrin signaling pathway affecting cellular adhesion and migration. Additionally JAML interacts with LFA-1 (Lymphocyte Function-associated Antigen 1) an integrin involved in leukocyte function modulating pathways that govern immune cell behavior and inflammation responses.

The functional role of JAML becomes particularly significant in contexts of inflammatory conditions and cancer. Inflammatory diseases such as inflammatory bowel disease (IBD) show considerable involvement of JAML as its regulatory influence on leukocyte transmigration exacerbates inflammation. Similarly changes in JAML expression have implications in cancer metastasis mechanisms as they relate to immune evasion in tumors. In these conditions JAML interacts closely with proteins like CAR and LFA-1 linking its expression and activity directly to disease progression and immune response modulation.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Transmembrane protein of the plasma membrane of leukocytes that control their migration and activation through interaction with CXADR, a plasma membrane receptor found on adjacent epithelial and endothelial cells. The interaction between both receptors mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, JAML induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair. It also controls the transmigration of leukocytes within epithelial and endothelial tissues through adhesive interactions with epithelial and endothelial CXADR.
See full target information JAML
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:9664 PubMed39511206

2024

mRNA delivery enabled by metal-organic nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Yuang Gu,Jingqu Chen,Zhaoran Wang,Chang Liu,Tianzheng Wang,Chan-Jin Kim,Helena Durikova,Soraia Fernandes,Darryl N Johnson,Robert De Rose,Christina Cortez-Jugo,Frank Caruso
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com