Anti-Jarid2 antibody [RP23040193]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Jarid2 antibody [RP23040193] (AB308119)

For immunofluorescence analysis, NCCIT cells were fixed and permeabilized for detection of Jarid2 using ab308119 at 2 µg/mL and labeled with Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution Panel a) shows representative cells that were stained for detection and localization of Jarid2 protein (green) Panel b) is stained for nuclei (blue) using DAPI Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin at 1/300 dilution Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of Jarid2 Panel e) represents control cells with no primary antibody to assess background The images were captured at 60X magnification

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Jarid2 antibody [RP23040193] (AB308119)

For immunofluorescence analysis, NTERA-2 cells were fixed and permeabilized for detection of Jarid2 using ab308119 at 2 µg/mL and labeled with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of Jarid2 protein (green) Panel b) is stained for nuclei (blue) using DAPI Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin at 1/300 dilution Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of Jarid2 Panel e) represents control cells with no primary antibody to assess background The images were captured at 60X magnification

• WB

Supplier Data

Western blot - Anti-Jarid2 antibody [RP23040193] (AB308119)

Western blot analysis was performed on Nuclear enriched extracts (30 µg lysate) of NTERA-2 (Lane 1), SH-SY 5Y (Lane 2), MOLT4 (Lane 3), K-562 (Lane 4), IMR32 (Lane 5), Neuro-2a (Lane 6), NCCIT (Lane 7) and HEK-293 (Lane 8). The blots were probed with ab308119 at 1-2 µg/mL and detected by chemiluminescence using a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution. A 139 kDa band corresponding to Jarid2 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane with wet transfer method. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed (ECL).

All lanes:

Western blot - Anti-Jarid2 antibody [RP23040193] (ab308119) at 1 µg/mL

Lane 1:

NTERA-2 cell lysate at 30 µg

Lane 2:

SH-SY5Y cell lysate at 30 µg

Lane 3:

MOLT4 cell lysate at 30 µg

Lane 4:

K562 cell lysate at 30 µg

Lane 5:

IMR32 cell lysate at 30 µg

Lane 6:

Neuro-2a cell lysate at 30 µg

Lane 7:

NCCIT cell lysate at 30 µg

Lane 8:

HEK-293 cell lysate at 30 µg

Secondary

All lanes:

HRP conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/2500 dilution

Observed band size: 139 kDa

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