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AB196761

Anti-JIP3 antibody

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(4 Publications)

Rabbit Polyclonal JIP3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MAPK8IP3.

View Alternative Names

JIP3, KIAA1066, MAPK8IP3, C-Jun-amino-terminal kinase-interacting protein 3, JIP-3, JNK-interacting protein 3, JNK MAP kinase scaffold protein 3, Mitogen-activated protein kinase 8-interacting protein 3

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-JIP3 antibody (AB196761)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-JIP3 antibody (AB196761)

Immunofluorescence analysis of HeLa cells labeling JIP3 using ab196761, at 1/100 dilution. Right panel was preincubated with blocking peptide.

Western blot - Anti-JIP3 antibody (AB196761)
  • WB

Supplier Data

Western blot - Anti-JIP3 antibody (AB196761)

All lanes:

Western blot - Anti-JIP3 antibody (ab196761) at 1/500 dilution

Lane 1:

HeLa cell extract

Lane 2:

HeLa cell extract with blocking peptide

Predicted band size: 147 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human MAPK8IP3. The exact immunogen used to generate this antibody is proprietary information.

Q9UPT6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

JIP3 also known as c-jun-amino-terminal kinase-interacting protein 3 or MAPK8IP3 is a scaffold protein with a molecular mass of approximately 150 kDa. It mediates intracellular signaling pathways and facilitates interactions between kinases and their substrates. JIP3 predominantly localizes in the cytoplasm and expresses highly in the brain especially in neurons. This protein plays a role in axonal transport and cytoskeletal organization.
Biological function summary

JIP3 influences neuronal signaling and development processes. It acts as a scaffold in kinase cascades particularly within the JNK signaling pathway forming complexes with JNK and other MAP kinases. The protein ensures proper localization and docking of kinases promoting efficient signal transduction. JIP3 links signaling components thereby influencing processes like neuronal migration and polarization.

Pathways

JIP3 serves as a significant component in the JNK and p38 MAPK pathways. These pathways are important for regulating cellular responses to stress apoptosis and immune responses. Within these pathways JIP3 interacts with JNK and other associated proteins like MAPK8 facilitating proper signaling flow. JIP3's function as a molecular scaffold coordinates cellular events through these signal transduction pathways.

JIP3 connects to neurodegenerative diseases such as Alzheimer’s and Amyotrophic Lateral Sclerosis (ALS). Dysregulation in the JNK signaling pathway often links to these conditions. Additionally disturbed axonal transport involving JIP3 can contribute to such neurodegenerative disorders. The relationship of JIP3 with other proteins like tau in Alzheimer’s further highlights the involvement of JIP3 in disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module (PubMed : 12189133). May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins (By similarity). Promotes neuronal axon elongation in a kinesin- and JNK-dependent manner. Activates cofilin at axon tips via local activation of JNK, thereby regulating filopodial dynamics and enhancing axon elongation. Its binding to kinesin heavy chains (KHC), promotes kinesin-1 motility along microtubules and is essential for axon elongation and regeneration. Regulates cortical neuronal migration by mediating NTRK2/TRKB anterograde axonal transport during brain development (By similarity). Acts as an adapter that bridges the interaction between NTRK2/TRKB and KLC1 and drives NTRK2/TRKB axonal but not dendritic anterograde transport, which is essential for subsequent BDNF-triggered signaling and filopodia formation (PubMed : 21775604).
See full target information MAPK8IP3
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 222: PubMed37909920

2023

Axonal transport of autophagosomes is regulated by dynein activators JIP3/JIP4 and ARF/RAB GTPases.

Applications

Unspecified application

Species

Unspecified reactive species

Sydney E Cason,Erika L F Holzbaur

eLife 11: PubMed36476638

2022

Doublecortin and JIP3 are neural-specific counteracting regulators of dynein-mediated retrograde trafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqin Fu,Lu Rao,Peijun Li,Xinglei Liu,Qi Wang,Alexander I Son,Arne Gennerich,Judy Shih-Hwa Liu

The Journal of cell biology 220: PubMed34014261

2021

Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sydney E Cason,Peter J Carman,Claire Van Duyne,Juliet Goldsmith,Roberto Dominguez,Erika L F Holzbaur

Molecular biology of the cell :mbcE19090530 PubMed33405953

2021

Doublecortin facilitates the elongation of the somatic Golgi apparatus into proximal dendrites.

Applications

Unspecified application

Species

Unspecified reactive species

Peijun Li,Luyao Li,Binyuan Yu,Xinye Wang,Qi Wang,Jingjing Lin,Yihui Zheng,Jinjin Zhu,Minzhi He,Zhaonan Xia,Mengjing Tu,Judy S Liu,Zhenlang Lin,Xiaoqin Fu
View all publications
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Product promise

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