AB12332

Anti-JLP antibody

Name: Anti-JLP antibody (ab12332) | Abcam
Brand: Abcam Limited
SKU: AB12332
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal JLP antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SPAG9 aa 750-850.

View Alternative Names

HSS, KIAA0516, MAPK8IP4, SYD1, HLC6, SPAG9, C-Jun-amino-terminal kinase-interacting protein 4, JIP-4, JNK-interacting protein 4, Cancer/testis antigen 89, Human lung cancer oncogene 6 protein, JNK-associated leucine-zipper protein, Mitogen-activated protein kinase 8-interacting protein 4, Proliferation-inducing protein 6, Protein highly expressed in testis, Sperm surface protein, Sperm-associated antigen 9, Sperm-specific protein, Sunday driver 1, CT89, HLC-6, JLP, PHET

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JLP antibody (AB12332)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JLP antibody (AB12332)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling JLP with ab12332 at 1/1000 (1µg/ml). Detection : DAB.

Supplier Data

Immunoprecipitation - Anti-JLP antibody (AB12332)

Immunoprecipitation analaysis of HeLa whole cell lysate (1 mg for IP; 20% of IP loaded). ab12332 was used for immunoprecipitation at 6 μg/mg lysate (lanes 1 and 2). Lane 3 - control IgG. For western blot ab12332 was used at 1 μg/ml.

All lanes:

Immunoprecipitation - Anti-JLP antibody (ab12332)

Predicted band size: 146 kDa

true

Exposure time: 30s

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Western blot - Anti-JLP antibody (AB12332)

Samples : Nuclear extract (NE, 20 μg) from HeLa cells. Antibody : Affinity purified rabbit anti-JLP antibody ab12332 used at 0.3 μg/ml. Detection : Chemiluminescence with an exposure time of 30 seconds.

Samples : Nuclear extract (NE, 20 µg) from HeLa cells. Antibody : Affinity purified rabbit anti-JLP antibody ab12332 used at 0.3 µg/ml. Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Western blot - Anti-JLP antibody (ab12332)

Predicted band size: 146 kDa

false

Supplier Data

Western blot - Anti-JLP antibody (AB12332)

All lanes:

Western blot - Anti-JLP antibody (ab12332) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Predicted band size: 146 kDa

true

Exposure time: 30s

Supplier Data

Western blot - Anti-JLP antibody (AB12332)

All lanes:

Western blot - Anti-JLP antibody (ab12332) at 0.1 µg/mL

Lane 1:

TCKM whole cell lysate at 50 µg

Lane 2:

Renca whole cell lysate at 15 µg

Lane 3:

CT26 whole cell lysate at 50 µg

Predicted band size: 146 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SPAG9 aa 750-850. The exact immunogen used to generate this antibody is proprietary information.

O60271

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "6 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Baboon": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody is affinity purified.

Storage buffer

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

JLP also known as JNK-associated leucine zipper protein is a scaffolding protein involved in the regulation of several signaling pathways. It has a molecular weight of approximately 155 kDa. JLP is widely expressed throughout various tissues including brain heart and skeletal muscle. It plays a role in directing components of signaling pathways to specific subcellular locations and works closely with other signaling proteins such as JIP4 (C-jun N-terminal kinase-interacting protein 4).

Biological function summary

JLP helps organize and maintain signaling complexes within the cell. It serves as a scaffold that brings together MAPK signaling components facilitating signal transduction and enhancing specificity in cell signaling events. By doing so it alters cellular responses to external stimuli. JLP often functions alongside other scaffold proteins within larger signaling groups promoting efficient signal cascade transmission and enabling precise regulation of cellular activities.

Pathways

JLP is particularly important within the mitogen-activated protein kinase (MAPK) pathway and the c-Jun N-terminal kinase (JNK) pathway. It aids in the organization and activation of these pathways by binding to specific kinases and other scaffolding proteins. JLP interacts with proteins like JNK and JIP1 to oversee the transmission of stress-related signals affecting processes such as apoptosis and inflammation.

JLP has connections to cancer and neurological disorders. Its involvement in cell signaling makes it a critical player in cancer progression as it can influence cellular growth and survival mechanisms. Alterations in JLP expression or function can disturb normal signaling balance therefore contributing to tumorigenesis. Additionally JLP’s function in neuronal signaling pathways links it to neurodegenerative diseases where dysregulated signaling could lead to disease pathology. Its interactions with proteins like JNK highlight its potential role in disease development offering a target for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module (PubMed : 14743216). Regulates lysosomal positioning by acting as an adapter protein which links PIP4P1-positive lysosomes to the dynein-dynactin complex (PubMed : 29146937). Assists PIKFYVE selective functionality in microtubule-based endosome-to-TGN trafficking (By similarity).

See full target information SPAG9

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical endocrinology and metaboli 94:2507-15 PubMed19351724

2009

Activated Ask1-MKK4-p38MAPK/JNK stress signaling pathway in human omental fat tissue may link macrophage infiltration to whole-body Insulin sensitivity.

Applications

Unspecified application

Species

Unspecified reactive species

Matthias Blüher,Nava Bashan,Iris Shai,Ilana Harman-Boehm,Tanya Tarnovscki,Eliezer Avinaoch,Michael Stumvoll,Arne Dietrich,Nora Klöting,Assaf Rudich

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-JLP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JLP antibody (AB12332)

Immunoprecipitation - Anti-JLP antibody (AB12332)

Western blot - Anti-JLP antibody (AB12332)

Western blot - Anti-JLP antibody (AB12332)

Western blot - Anti-JLP antibody (AB12332)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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