Rabbit Recombinant Monoclonal JLP antibody. Carrier free. Suitable for IP, WB and reacts with Human, Rat, Mouse samples.
Images
![Western blot - Anti-JLP antibody [EPR25121-32] - BSA and Azide free (AB307427), expandable thumbnail](https://content.abcam.com/products/images/jlp-antibody-epr25121-32-bsa-and-azide-free-ab307427--western-blot-img405576.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-JLP antibody [EPR25121-32] - BSA and Azide free (AB307427)")
![Western blot - Anti-JLP antibody [EPR25121-32] - BSA and Azide free (AB307427), expandable thumbnail](https://content.abcam.com/products/images/jlp-antibody-epr25121-32-bsa-and-azide-free-ab307427--western-blot-img405577.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-JLP antibody [EPR25121-32] - BSA and Azide free (AB307427)")
![Western blot - Anti-JLP antibody [EPR25121-32] - BSA and Azide free (AB307427), expandable thumbnail](https://content.abcam.com/products/images/jlp-antibody-epr25121-32-bsa-and-azide-free-ab307427--western-blot-img405578.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-JLP antibody [EPR25121-32] - BSA and Azide free (AB307427)")
![Immunoprecipitation - Anti-JLP antibody [EPR25121-32] - BSA and Azide free (AB307427), expandable thumbnail](https://content.abcam.com/products/images/jlp-antibody-epr25121-32-bsa-and-azide-free-ab307427--immunoprecipitation-img406403.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-JLP antibody [EPR25121-32] - BSA and Azide free (AB307427)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt (Intra) | ICC/IF | IHC-P | WB | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
| Rat | Expected | Not recommended | Not recommended | Not recommended | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Target data
Function
The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module (PubMed:14743216). Regulates lysosomal positioning by acting as an adapter protein which links PIP4P1-positive lysosomes to the dynein-dynactin complex (PubMed:29146937). Assists PIKFYVE selective functionality in microtubule-based endosome-to-TGN trafficking (By similarity).
Alternative names
HSS, KIAA0516, MAPK8IP4, SYD1, HLC6, SPAG9, C-Jun-amino-terminal kinase-interacting protein 4, JIP-4, JNK-interacting protein 4, Cancer/testis antigen 89, Human lung cancer oncogene 6 protein, JNK-associated leucine-zipper protein, Mitogen-activated protein kinase 8-interacting protein 4, Proliferation-inducing protein 6, Protein highly expressed in testis, Sperm surface protein, Sperm-associated antigen 9, Sperm-specific protein, Sunday driver 1, CT89, HLC-6, JLP, PHET
Recommended products
Rabbit Recombinant Monoclonal JLP antibody. Carrier free. Suitable for IP, WB and reacts with Human, Rat, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25121-32
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
JLP also known as JNK-associated leucine zipper protein is a scaffolding protein involved in the regulation of several signaling pathways. It has a molecular weight of approximately 155 kDa. JLP is widely expressed throughout various tissues including brain heart and skeletal muscle. It plays a role in directing components of signaling pathways to specific subcellular locations and works closely with other signaling proteins such as JIP4 (C-jun N-terminal kinase-interacting protein 4).
- Biological function summary
JLP helps organize and maintain signaling complexes within the cell. It serves as a scaffold that brings together MAPK signaling components facilitating signal transduction and enhancing specificity in cell signaling events. By doing so it alters cellular responses to external stimuli. JLP often functions alongside other scaffold proteins within larger signaling groups promoting efficient signal cascade transmission and enabling precise regulation of cellular activities.
- Pathways
JLP is particularly important within the mitogen-activated protein kinase (MAPK) pathway and the c-Jun N-terminal kinase (JNK) pathway. It aids in the organization and activation of these pathways by binding to specific kinases and other scaffolding proteins. JLP interacts with proteins like JNK and JIP1 to oversee the transmission of stress-related signals affecting processes such as apoptosis and inflammation.
- Associated diseases and disorders
JLP has connections to cancer and neurological disorders. Its involvement in cell signaling makes it a critical player in cancer progression as it can influence cellular growth and survival mechanisms. Alterations in JLP expression or function can disturb normal signaling balance therefore contributing to tumorigenesis. Additionally JLP’s function in neuronal signaling pathways links it to neurodegenerative diseases where dysregulated signaling could lead to disease pathology. Its interactions with proteins like JNK highlight its potential role in disease development offering a target for therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-JLP antibody [EPR25121-32] - BSA and Azide free (ab307427), expandable thumbnail](https://content.abcam.com/products/images/jlp-antibody-epr25121-32-bsa-and-azide-free-ab307427--western-blot-img405576.jpg "Western blot - Anti-JLP antibody [EPR25121-32] - BSA and Azide free (ab307427)")
Western blot - Anti-JLP antibody [EPR25121-32] - BSA and Azide free (ab307427)
This data was developed using 307426, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Negative control: A-172 (PMID: 23696027).
70 seconds
Exposure time:All lanes: Western blot - Anti-JLP antibody [EPR25121-32] (Anti-JLP antibody [EPR25121-32] ab307426) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate 20 μg
Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate 20 μg
Lane 3: A-172 (human brain glioblastoma cell ) whole cell lysate 20 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 190 kDa
Exposure time: 70s
![Western blot - Anti-JLP antibody [EPR25121-32] - BSA and Azide free (ab307427), expandable thumbnail](https://content.abcam.com/products/images/jlp-antibody-epr25121-32-bsa-and-azide-free-ab307427--western-blot-img405577.jpg "Western blot - Anti-JLP antibody [EPR25121-32] - BSA and Azide free (ab307427)")
Western blot - Anti-JLP antibody [EPR25121-32] - BSA and Azide free (ab307427)
This data was developed using 307426, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
15 seconds
Exposure time:All lanes: Western blot - Anti-JLP antibody [EPR25121-32] (Anti-JLP antibody [EPR25121-32] ab307426) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate 20 μg
Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate 20 μg
Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 20 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 190 kDa
Exposure time: 15s
![Western blot - Anti-JLP antibody [EPR25121-32] - BSA and Azide free (ab307427), expandable thumbnail](https://content.abcam.com/products/images/jlp-antibody-epr25121-32-bsa-and-azide-free-ab307427--western-blot-img405578.jpg "Western blot - Anti-JLP antibody [EPR25121-32] - BSA and Azide free (ab307427)")
Western blot - Anti-JLP antibody [EPR25121-32] - BSA and Azide free (ab307427)
This data was developed using 307426, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
48 seconds
Exposure time:All lanes: Western blot - Anti-JLP antibody [EPR25121-32] (Anti-JLP antibody [EPR25121-32] ab307426) at 1/1000 dilution
Lane 1: Mouse testis tissue lysate 20 μg
Lane 2: Rat testis tissue lysate 20 μg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 190 kDa
Exposure time: 48s
![Immunoprecipitation - Anti-JLP antibody [EPR25121-32] - BSA and Azide free (ab307427), expandable thumbnail](https://content.abcam.com/products/images/jlp-antibody-epr25121-32-bsa-and-azide-free-ab307427--immunoprecipitation-img406403.jpg "Immunoprecipitation - Anti-JLP antibody [EPR25121-32] - BSA and Azide free (ab307427)")
Immunoprecipitation - Anti-JLP antibody [EPR25121-32] - BSA and Azide free (ab307427)
This data was developed using Anti-JLP antibody [EPR25121-32] ab307426, the same antibody clone in a different buffer formulation.
JLP was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) whole cell lysate 10 ug with Anti-JLP antibody [EPR25121-32] ab307426 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-JLP antibody [EPR25121-32] ab307426 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: 293T (human embryonic kidney epithelial cell) whole cell lysate 10 ug
Lane 2: ABAB307426 IP in 293T whole cell lysate
Lane 3:RABbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-JLP antibody [EPR25121-32] ab307426 in 293T whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds
Lysates were freshly made and used for IP immediately to minimize protein degradation.All lanes: Immunoprecipitation - Anti-JLP antibody [EPR25121-32] (Anti-JLP antibody [EPR25121-32] ab307426) at 1/1000 dilution
Lane 1: 293T (human embryonic kidney epithelial cell) whole cell lysate 10 μg
Lane 2: 293T whole cell lysate
Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 190 kDa
Exposure time: 3s
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