AB157586

Anti-JMY antibody - C-terminal

Name: Anti-JMY antibody - C-terminal (ab157586) | Abcam
Brand: Abcam Limited
SKU: AB157586
Price: 535 USD
Availability: InStock

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal JMY antibody. Suitable for WB, ICC/IF and reacts with Rat, Human, Mouse, Chicken samples. Cited in 1 publication.

View Alternative Names

Junction-mediating and -regulatory protein, JMY

2 Images

Unknown

Western blot - Anti-JMY antibody - C-terminal (AB157586)

All lanes:

Western blot - Anti-JMY antibody - C-terminal (ab157586) at 1/500 dilution

Lane 1:

Rat PC12 cell lysate

Lane 2:

Jurkat cell lysate

Lane 3:

Adult Mouse heart lysate

Predicted band size: 111 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-JMY antibody - C-terminal (AB157586)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-JMY antibody - C-terminal (AB157586)

Immunofluorescent analysis of chick fibroblasts labeling JMY with ab157586 at 1/200 dilution (green); F-Actin (red).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Chicken, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen has low homology to other WASP family members.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "" }, "Frog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

JMY also known as Junction-mediating and -regulatory protein is a protein involved in actin nucleation. It interacts with actin to facilitate the formation of new actin filaments. JMY has a molecular mass of around 168 kDa and is highly expressed in various tissues including the brain heart and skeletal muscle. This expression pattern suggests its widespread role in cellular processes across different organ systems.

Biological function summary

JMY influences cell movement and stability by controlling actin polymerization. It contributes to the actin-related protein (Arp2/3) complex which is important for branching actin filament networks. JMY controls cell migration and morphology through its actin-nucleating activity affecting how cells respond to their environment and communicate with one another. Its function in actin dynamics makes it a significant player in maintaining cellular architecture and facilitating processes like endocytosis and phagocytosis.

Pathways

JMY participates in the regulation of the actin cytoskeleton and p53 pathways. In the actin cytoskeleton pathway JMY interacts with other proteins like WAVE and Arp2/3 to control actin assembly impacting cell shape and motility. In the p53 pathway JMY modulates transcriptional responses to DNA damage linking its actin-regulating functions with cellular stress responses. JMY establishes important connections between signaling pathways and structural cellular components therefore influencing a range of cellular behaviors.

JMY has been implicated in cancer progression and neurodegenerative diseases. Its dysregulation can lead to altered cell migration and invasion which are characteristics of cancer metastasis. For example loss of JMY activity might relate to the ability of cancer cells to metastasize especially in breast cancer. In neurodegenerative diseases aberrant actin dynamics potentially involving JMY contribute to neuronal dysfunction and loss. Research continues to explore JMY's interaction with other proteins in disease contexts including p53 pointing to its dual regulatory role in both structural integrity and gene transcription.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions (PubMed : 30420355). In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments (PubMed : 30420355). Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments (PubMed : 30420355). Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. Upon nutrient stress, directly recruited by MAP1LC3B to the phagophore membrane surfaces to promote actin assembly during autophagy (PubMed : 30420355). The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location (By similarity).

See full target information Junction-mediating and -regulatory protein

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 58:1084-1097 PubMed28192799

2017

Cyp1b1 Regulates Ocular Fissure Closure Through a Retinoic Acid-Independent Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Antionette L Williams,Jessica Eason,Bahaar Chawla,Brenda L Bohnsack

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-JMY antibody - C-terminal

Western blot - Anti-JMY antibody - C-terminal (AB157586)

Immunocytochemistry/ Immunofluorescence - Anti-JMY antibody - C-terminal (AB157586)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise