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Rabbit Polyclonal JMY antibody. Suitable for WB, ICC/IF and reacts with Rat, Human, Mouse, Chicken samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-JMY antibody - C-terminal (AB157586), expandable thumbnail
  • Western blot - Anti-JMY antibody - C-terminal (AB157586), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Expected
Mouse
Tested
Expected
Rat
Tested
Expected
Chicken
Expected
Tested
Frog
Predicted
Predicted

Tested
Tested

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Chicken

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Frog

Dilution info

-

Notes

-

Tested
Tested

Species

Chicken

Dilution info

1/200

Notes

-

Expected
Expected

Species

Rat, Human, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Frog

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions (PubMed:30420355). In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments (PubMed:30420355). Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments (PubMed:30420355). Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. Upon nutrient stress, directly recruited by MAP1LC3B to the phagophore membrane surfaces to promote actin assembly during autophagy (PubMed:30420355). The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location (By similarity).

Alternative names

Recommended products

Rabbit Polyclonal JMY antibody. Suitable for WB, ICC/IF and reacts with Rat, Human, Mouse, Chicken samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique

Affinity purification Immunogen

Specificity

The immunogen has low homology to other WASP family members.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

JMY also known as Junction-mediating and -regulatory protein is a protein involved in actin nucleation. It interacts with actin to facilitate the formation of new actin filaments. JMY has a molecular mass of around 168 kDa and is highly expressed in various tissues including the brain heart and skeletal muscle. This expression pattern suggests its widespread role in cellular processes across different organ systems.

Biological function summary

JMY influences cell movement and stability by controlling actin polymerization. It contributes to the actin-related protein (Arp2/3) complex which is important for branching actin filament networks. JMY controls cell migration and morphology through its actin-nucleating activity affecting how cells respond to their environment and communicate with one another. Its function in actin dynamics makes it a significant player in maintaining cellular architecture and facilitating processes like endocytosis and phagocytosis.

Pathways

JMY participates in the regulation of the actin cytoskeleton and p53 pathways. In the actin cytoskeleton pathway JMY interacts with other proteins like WAVE and Arp2/3 to control actin assembly impacting cell shape and motility. In the p53 pathway JMY modulates transcriptional responses to DNA damage linking its actin-regulating functions with cellular stress responses. JMY establishes important connections between signaling pathways and structural cellular components therefore influencing a range of cellular behaviors.

Associated diseases and disorders

JMY has been implicated in cancer progression and neurodegenerative diseases. Its dysregulation can lead to altered cell migration and invasion which are characteristics of cancer metastasis. For example loss of JMY activity might relate to the ability of cancer cells to metastasize especially in breast cancer. In neurodegenerative diseases aberrant actin dynamics potentially involving JMY contribute to neuronal dysfunction and loss. Research continues to explore JMY's interaction with other proteins in disease contexts including p53 pointing to its dual regulatory role in both structural integrity and gene transcription.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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2 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-JMY antibody - C-terminal (ab157586), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-JMY antibody - C-terminal (ab157586)

    Immunofluorescent analysis of chick fibroblasts labeling JMY with ab157586 at 1/200 dilution (green); F-Actin (red).

  • Western blot - Anti-JMY antibody - C-terminal (ab157586), expandable thumbnail

    Western blot - Anti-JMY antibody - C-terminal (ab157586)

    All lanes: Western blot - Anti-JMY antibody - C-terminal (ab157586) at 1/500 dilution

    Lane 1: Rat PC12 cell lysate

    Lane 2: Jurkat cell lysate

    Lane 3: Adult Mouse heart lysate

    Predicted band size: 111 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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