JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB190212

Anti-JMY antibody - C-terminal

Be the first to review this product! Submit a review

|

(3 Publications)

Rabbit Polyclonal JMY antibody. C-terminal. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human Junction-mediating and -regulatory protein aa 900 to C-terminus.

View Alternative Names

Junction-mediating and -regulatory protein, JMY

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JMY antibody - C-terminal (AB190212)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JMY antibody - C-terminal (AB190212)

Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human brain, cortex, tissue labeling JMY using ab190212 at 10 μg/ml.

Western blot - Anti-JMY antibody - C-terminal (AB190212)
  • WB

Supplier Data

Western blot - Anti-JMY antibody - C-terminal (AB190212)

All lanes:

Western blot - Anti-JMY antibody - C-terminal (ab190212) at 1/500 dilution

Lane 1:

COLO cell lysate

Lane 2:

COLO cell lysate with Synthesized peptide

Predicted band size: 111 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human Junction-mediating and -regulatory protein aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8N9B5

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

JMY also known as Junction-mediating and -regulatory protein is a protein involved in actin nucleation. It interacts with actin to facilitate the formation of new actin filaments. JMY has a molecular mass of around 168 kDa and is highly expressed in various tissues including the brain heart and skeletal muscle. This expression pattern suggests its widespread role in cellular processes across different organ systems.
Biological function summary

JMY influences cell movement and stability by controlling actin polymerization. It contributes to the actin-related protein (Arp2/3) complex which is important for branching actin filament networks. JMY controls cell migration and morphology through its actin-nucleating activity affecting how cells respond to their environment and communicate with one another. Its function in actin dynamics makes it a significant player in maintaining cellular architecture and facilitating processes like endocytosis and phagocytosis.

Pathways

JMY participates in the regulation of the actin cytoskeleton and p53 pathways. In the actin cytoskeleton pathway JMY interacts with other proteins like WAVE and Arp2/3 to control actin assembly impacting cell shape and motility. In the p53 pathway JMY modulates transcriptional responses to DNA damage linking its actin-regulating functions with cellular stress responses. JMY establishes important connections between signaling pathways and structural cellular components therefore influencing a range of cellular behaviors.

JMY has been implicated in cancer progression and neurodegenerative diseases. Its dysregulation can lead to altered cell migration and invasion which are characteristics of cancer metastasis. For example loss of JMY activity might relate to the ability of cancer cells to metastasize especially in breast cancer. In neurodegenerative diseases aberrant actin dynamics potentially involving JMY contribute to neuronal dysfunction and loss. Research continues to explore JMY's interaction with other proteins in disease contexts including p53 pointing to its dual regulatory role in both structural integrity and gene transcription.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions (PubMed : 30420355). In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments (PubMed : 30420355). Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments (PubMed : 30420355). Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. Upon nutrient stress, directly recruited by MAP1LC3B to the phagophore membrane surfaces to promote actin assembly during autophagy (PubMed : 30420355). The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location (By similarity).
See full target information Junction-mediating and -regulatory protein
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

STAR protocols 3:101374 PubMed35586313

2022

ChipCytometry for multiplexed detection of protein and mRNA markers on human FFPE tissue samples.

Applications

Unspecified application

Species

Unspecified reactive species

Sebastian Jarosch,Jan Köhlen,Sabrina Wagner,Elvira D'Ippolito,Dirk H Busch

Orthopaedic surgery 12:964-973 PubMed32363797

2020

Role of Junction-Mediating and Regulatory Protein in the Pathogenesis of Glucocorticoid-Induced Endothelial Cell Lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zuo,Wan-Shou Guo,Hua-Chen Yu,Pei Liu,Qi-Dong Zhang

The FEBS journal 287:5478-5497 PubMed32279424

2020

JMY expression by Sertoli cells contributes to mediating spermatogenesis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Liu,Jiaying Fan,Yan Yan,Xuening Dang,Ran Zhao,Yimei Xu,Zhide Ding
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB157586

Anti-JMY antibody - C-terminal

Western blot - Anti-JMY antibody - C-terminal (AB157586)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB122572

Anti-WHAMM antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WHAMM antibody (AB122572)

  • 0
  • 0
Proteins & Peptides

AB127477

Recombinant Human JMY protein

  • 0
  • 0
Primary Antibodies

AB191536

Anti-PAG608 antibody

Western blot - Anti-PAG608 antibody (AB191536)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com