Anti-JNK1 antibody [EPR140(2)]

• WB

Lab

Western blot - Anti-JNK1 antibody [EPR140(2)] (AB110724)

All lanes:

Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724) at 1/2000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

Lane 3:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 48 kDa

Observed band size: 46 kDa,54 kDa

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• WB

Unknown

Western blot - Anti-JNK1 antibody [EPR140(2)] (AB110724)

All lanes:

Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

MCF7 cell lysate at 10 µg

Predicted band size: 48 kDa

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• WB

Lab

Western blot - Anti-JNK1 antibody [EPR140(2)] (AB110724)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : JNK1 knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : MCF7 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab110724 observed at 46 and 54 kDa. Red - loading control, ab8226, observed at 42 kDa.

ab110724 (unpurified) was shown to specifically react with JNK1 when JNK1 knockout samples were used. Wild-type and ProteinX knockout samples were subjected to SDS-PAGE. ab110724 and ab8226 (loading control to beta actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)

Predicted band size: 48 kDa

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• WB

Lab

Western blot - Anti-JNK1 antibody [EPR140(2)] (AB110724)

False colour image of Western blot : Anti-JNK1 antibody [EPR140(2)] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab110724 was shown to bind specifically to JNK1. A band was observed at 42/48 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in mapk8 knockout cell line ab277181 (knockout cell lysate ab277223). To generate this image, wild-type and mapk8 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724) at 1/1000 dilution

Lane 1:

Wild-type U-2 OS cell lysate at 20 µg

Lane 2:

MAPK8 knockout U-2 OS cell lysate at 20 µg

Observed band size: 42-48 kDa

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• WB

Lab

Western blot - Anti-JNK1 antibody [EPR140(2)] (AB110724)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : JNK1 knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : MCF7 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green).

Green - target observed at 46 and 54 kDa. Red - loading control, ab8226, observed at 42 kDa.

This western blot image is a comparison between ab110724 and a competitor's top cited mouse monoclonal antibody.

All lanes:

Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)

Predicted band size: 48 kDa

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• WB

Lab

Western blot - Anti-JNK1 antibody [EPR140(2)] (AB110724)

Western blot : Rabbit Monoclonal [EPR140(2)] to JNK1 ab110724 staining at 1/1000 dilution, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta.

A band was observed at 42 kDa in Wild-type A549 cell lysates with no signal observed at this size in MAPK8 knockout A549 cell line.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.

Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

MAPK8 knockout A549 at 20 µg

Lane 2:

Western blot - Human MAPK8 knockout A549 cell line (ab286604) at 20 µg

Lane 3:

Wild-type U-2 OS at 20 µg

Lane 4:

MAPK8 knockout U-2 OS at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 48 kDa

Observed band size: 42 kDa

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