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Knockout Tested Rabbit Recombinant Monoclonal JNK1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free (AB247935), expandable thumbnail
  • Western blot - Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free (AB247935), expandable thumbnail
  • Western blot - Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free (AB247935), expandable thumbnail
  • Western blot - Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free (AB247935), expandable thumbnail
  • Western blot - Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free (AB247935), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway (PubMed:28943315). In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity (PubMed:18307971). Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins (PubMed:21856198). Loss of this interaction abrogates the acetylation required for replication initiation (PubMed:21856198). Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1 (PubMed:21364637). In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation (PubMed:21095239). Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy (PubMed:18570871). Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons (By similarity). In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone (By similarity). Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH (PubMed:16581800, PubMed:17296730, PubMed:20027304). Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692). Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity (PubMed:10747973). Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteasomal degradation (By similarity). Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1 (PubMed:22327296). In neurons, phosphorylates SYT4 which captures neuronal dense core vesicles at synapses (By similarity). Phosphorylates EIF4ENIF1/4-ET in response to oxidative stress, promoting P-body assembly (PubMed:22966201). Phosphorylates SIRT6 in response to oxidative stress, stimulating its mono-ADP-ribosyltransferase activity (PubMed:27568560). Phosphorylates NLRP3, promoting assembly of the NLRP3 inflammasome (PubMed:28943315). Phosphorylates ALKBH5 in response to reactive oxygen species (ROS), promoting ALKBH5 sumoylation and inactivation (PubMed:34048572). JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

Alternative names

JNK1, PRKM8, SAPK1, SAPK1C, MAPK8, Mitogen-activated protein kinase 8, MAP kinase 8, MAPK 8, JNK-46, Stress-activated protein kinase 1c, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, SAPK1c

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Knockout Tested Rabbit Recombinant Monoclonal JNK1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR140(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab247935 is the carrier-free version of Anti-JNK1 antibody [EPR140(2)] ab110724.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

JNK1 also known as c-Jun N-terminal kinase 1 is a member of the mitogen-activated protein kinase (MAPK) family with a molecular weight of approximately 46 kDa. It is expressed in various tissues throughout the body including the brain heart liver and skeletal muscle. JNK1 exists in multiple isoforms due to alternative splicing. The JNK1 protein is activated by dual phosphorylation on threonine and tyrosine residues a process integral to its function as a stress-activated protein kinase. Commonly used research tools include JNK antibodies and phospho JNK antibodies which help detect the activated forms of this kinase during cellular studies.

Biological function summary

This kinase plays an important role in processes such as inflammation apoptosis and cellular stress responses. JNK1 is not just an isolated enzyme. It forms complexes with other proteins under specific conditions facilitating diverse cellular responses. For example JNK1 activation influences transcription factors like c-Jun by phosphorylating them impacting gene expression related to cell survival and death. This activity establishes JNK1 as a significant player in routine cell functioning and response to external environmental stressors.

Pathways

The kinase is part of the MAPK signaling pathways and the stress-activated protein kinase (SAPK) pathways. These pathways involve multiple signaling cascades important for transmitting extracellular signals into the cellular environment. JNK1 interacts with proteins like MKK4 and MKK7 which are upstream activators and ATF2 a downstream target. This positioning makes JNK1 an essential signaling node that translates extracellular stressors into cellular responses providing adaptability to cells amidst changing conditions.

Associated diseases and disorders

JNK1 has connections to disorders such as cancer and neurodegenerative diseases. Abnormal activation of JNK1 can lead to irregular cell proliferation making it pertinent in cancer. Similarly in neurodegenerative diseases like Alzheimer's JNK1's involvement in neuronal apoptosis turns critical. It regulates tau phosphorylation connecting JNK1 with tauopathies observed in Alzheimer's disease. Researchers often investigate these associations to understand the mechanisms that underpin these conditions and develop targeted therapies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free (ab247935), expandable thumbnail

    Western blot - Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free (ab247935)

    This data was developed using Anti-JNK1 antibody [EPR140(2)] ab110724, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 μg)

    Lane 2: JNK1 knockout HAP1 cell lysate (20 μg)

    Lane 3: HeLa cell lysate (20 μg)

    Lane 4: MCF7 cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - Anti-JNK1 antibody [EPR140(2)] ab110724 observed at 46 and 54 kDa. Red - loading control, Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226, observed at 42 kDa.

    Anti-JNK1 antibody [EPR140(2)] ab110724 (unpurified) was shown to specifically react with JNK1 when JNK1 knockout samples were used. Wild-type and ProteinX knockout samples were subjected to SDS-PAGE. Anti-JNK1 antibody [EPR140(2)] ab110724 and Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226 (loading control to beta actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-JNK1 antibody [EPR140(2)] (Anti-JNK1 antibody [EPR140(2)] ab110724)

    Predicted band size: 48 kDa

  • Western blot - Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free (ab247935), expandable thumbnail

    Western blot - Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free (ab247935)

    This data was developed using Anti-JNK1 antibody [EPR140(2)] ab110724, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-JNK1 antibody [EPR140(2)] (Anti-JNK1 antibody [EPR140(2)] ab110724) at 1/2000 dilution

    Lane 1: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 2: C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

    Lane 3: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 48 kDa

    Observed band size: 46 kDa, 54 kDa

  • Western blot - Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free (ab247935), expandable thumbnail

    Western blot - Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free (ab247935)

    This data was developed using Anti-JNK1 antibody [EPR140(2)] ab110724, the same antibody clone in a different buffer formulation.

    Lane 1 Wild-type HAP1 cell lysate (20 μg)

    Lane 2 JNK1 knockout HAP1 cell lysate (20 μg)

    Lane 3 HeLa cell lysate (20 μg)

    Lane 4 MCF7 cell lysate (20 μg)

    Lanes 1 - 4 Merged signal (red and green).

    Green - target observed at 46 and 54 kDa. Red - loading control, Anti-beta Actin antibody [mAbcam 8226] - Loading Control ab8226, observed at 42 kDa.

    This western blot image is a comparison between Anti-JNK1 antibody [EPR140(2)] ab110724 and a competitor's top cited mouse monoclonal antibody.

    All lanes: Western blot - Anti-JNK1 antibody [EPR140(2)] (Anti-JNK1 antibody [EPR140(2)] ab110724)

    Predicted band size: 48 kDa

  • Western blot - Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free (ab247935), expandable thumbnail

    Western blot - Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free (ab247935)

    This data was developed using Anti-JNK1 antibody [EPR140(2)] ab110724, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-JNK1 antibody [EPR140(2)] (Anti-JNK1 antibody [EPR140(2)] ab110724) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: 293T cell lysate at 10 µg

    Lane 3: K562 cell lysate at 10 µg

    Lane 4: MCF7 cell lysate at 10 µg

    Predicted band size: 48 kDa

  • Western blot - Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free (ab247935), expandable thumbnail

    Western blot - Anti-JNK1 antibody [EPR140(2)] - BSA and Azide free (ab247935)

    False colour image of Western blot: Anti-JNK1 antibody [EPR140(2)] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-JNK1 antibody [EPR140(2)] ab110724 was shown to bind specifically to JNK1. A band was observed at 42/48 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in mapk8 knockout cell line Human MAPK8 knockout U-2 OS cell line ab277181 (knockout cell lysate ab277223). To generate this image, wild-type and mapk8 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-JNK1 antibody [EPR140(2)] (Anti-JNK1 antibody [EPR140(2)] ab110724) at 1/1000 dilution

    Lane 1: Wild-type U-2 OS cell lysate at 20 µg

    Lane 2: MAPK8 knockout U-2 OS cell lysate at 20 µg

    Performed under reducing conditions.

    Observed band size: 42-48 kDa

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