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AB251542

Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free

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Rabbit Recombinant Monoclonal JNK1 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples.

View Alternative Names

JNK1, PRKM8, SAPK1, SAPK1C, MAPK8, Mitogen-activated protein kinase 8, MAP kinase 8, MAPK 8, JNK-46, Stress-activated protein kinase 1c, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, SAPK1c

7 Images
Western blot - Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (AB251542)
  • WB

Lab

Western blot - Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (AB251542)

This data was developed using ab213521, the same antibody clone in a different buffer formulation.

Western blot : Rabbit Monoclonal [EPR18841-99] to JNK1 ab213521 staining at 1/1000 dilution, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta.

A band was observed at 42 kDa in Wild-type A549 cell lysates with no signal observed at this size in MAPK8 knockout A549 cell line. We are unable to identify the non-specific band at 60kDa.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.

Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-JNK1 antibody [EPR18841-99] (<a href='/en-us/products/primary-antibodies/jnk1-antibody-epr18841-99-ab213521'>ab213521</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

MAPK8 knockout A549 at 20 µg

Lane 2:

Western blot - Human MAPK8 knockout A549 cell line (ab286604) at 20 µg

Lane 3:

Wild-type U-2 OS at 20 µg

Lane 4:

MAPK8 knockout U-2 OS at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 48 kDa

Observed band size: 42 kDa

false

Immunoprecipitation - Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (AB251542)
  • IP

Supplier Data

Immunoprecipitation - Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (AB251542)

This data was developed using ab213521, the same antibody clone in a different buffer formulation.

JNK1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab213521 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab213521 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1 : HeLa whole cell lysate, 10ug (Input).
Lane 2 : ab213521 IP in HeLa whole cell lysate.
Lane 3 : Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab213521 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-JNK1 antibody [EPR18841-99] (<a href='/en-us/products/primary-antibodies/jnk1-antibody-epr18841-99-ab213521'>ab213521</a>)

Predicted band size: 48 kDa

false

Western blot - Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (AB251542)
  • WB

Supplier Data

Western blot - Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (AB251542)

This data was developed using ab213521, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-JNK1 antibody [EPR18841-99] (<a href='/en-us/products/primary-antibodies/jnk1-antibody-epr18841-99-ab213521'>ab213521</a>) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg

Lane 2:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg with Human JNK1 recombinant protein fragment (immunogen).

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 48 kDa

Observed band size: 46-54 kDa

false

Exposure time: 3s

Western blot - Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (AB251542)
  • WB

Supplier Data

Western blot - Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (AB251542)

This data was developed using ab213521, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1 : 1 second; Lanes 2-3 : 3 minutes.

Human JNK1 full length recombinant protein contain aa1-427 with a His-Tag®. Human JNK2 full length recombinant protein contain aa1-424 with a His-Tag®. Human JNK3 full length recombinant protein contain aa1-464 with a His-Tag®.

All lanes:

Western blot - Anti-JNK1 antibody [EPR18841-99] (<a href='/en-us/products/primary-antibodies/jnk1-antibody-epr18841-99-ab213521'>ab213521</a>) at 1/1000 dilution

Lane 1:

Human JNK1 full length recombinant protein at 0.01 µg

Lane 2:

Human JNK2 full length recombinant protein at 0.01 µg

Lane 3:

Human JNK3 full length recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa

false

Western blot - Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (AB251542)
  • WB

Supplier Data

Western blot - Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (AB251542)

This data was developed using ab213521, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : Lanes 1-7 : 3 minutes; Lane 8-11 : 15 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID : 11313405).

All lanes:

Western blot - Anti-JNK1 antibody [EPR18841-99] (<a href='/en-us/products/primary-antibodies/jnk1-antibody-epr18841-99-ab213521'>ab213521</a>) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse kidney lysate at 10 µg

Lane 3:

Mouse spleen lysate at 10 µg

Lane 4:

Rat brain lysate at 10 µg

Lane 5:

Rat heart lysate at 10 µg

Lane 6:

Rat kidney lysate at 10 µg

Lane 7:

Rat spleen lysate at 10 µg

Lane 8:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 9:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 10:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 11:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 48 kDa

Observed band size: 46-54 kDa

false

Western blot - Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (AB251542)
  • WB

Supplier Data

Western blot - Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (AB251542)

This data was developed using ab213521, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The molecular weight bserved is consistent with what has been described in the literature (PMID : 11313405).

All lanes:

Western blot - Anti-JNK1 antibody [EPR18841-99] (<a href='/en-us/products/primary-antibodies/jnk1-antibody-epr18841-99-ab213521'>ab213521</a>) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg

Lane 4:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 48 kDa

Observed band size: 46-54 kDa

false

Exposure time: 3min

Western blot - Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (AB251542)
  • WB

Supplier Data

Western blot - Anti-JNK1 antibody [EPR18841-99] - BSA and Azide free (AB251542)

This data was developed using ab213521, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 11313405).

All lanes:

Western blot - Anti-JNK1 antibody [EPR18841-99] (<a href='/en-us/products/primary-antibodies/jnk1-antibody-epr18841-99-ab213521'>ab213521</a>) at 1/1000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

Human fetal heart tissue lysate at 10 µg

Lane 3:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 48 kDa

Observed band size: 46-54 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18841-99

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251542 is the carrier-free version of ab213521.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

JNK1 also known as c-Jun N-terminal kinase 1 is a member of the mitogen-activated protein kinase (MAPK) family with a molecular weight of approximately 46 kDa. It is expressed in various tissues throughout the body including the brain heart liver and skeletal muscle. JNK1 exists in multiple isoforms due to alternative splicing. The JNK1 protein is activated by dual phosphorylation on threonine and tyrosine residues a process integral to its function as a stress-activated protein kinase. Commonly used research tools include JNK antibodies and phospho JNK antibodies which help detect the activated forms of this kinase during cellular studies.
Biological function summary

This kinase plays an important role in processes such as inflammation apoptosis and cellular stress responses. JNK1 is not just an isolated enzyme. It forms complexes with other proteins under specific conditions facilitating diverse cellular responses. For example JNK1 activation influences transcription factors like c-Jun by phosphorylating them impacting gene expression related to cell survival and death. This activity establishes JNK1 as a significant player in routine cell functioning and response to external environmental stressors.

Pathways

The kinase is part of the MAPK signaling pathways and the stress-activated protein kinase (SAPK) pathways. These pathways involve multiple signaling cascades important for transmitting extracellular signals into the cellular environment. JNK1 interacts with proteins like MKK4 and MKK7 which are upstream activators and ATF2 a downstream target. This positioning makes JNK1 an essential signaling node that translates extracellular stressors into cellular responses providing adaptability to cells amidst changing conditions.

JNK1 has connections to disorders such as cancer and neurodegenerative diseases. Abnormal activation of JNK1 can lead to irregular cell proliferation making it pertinent in cancer. Similarly in neurodegenerative diseases like Alzheimer's JNK1's involvement in neuronal apoptosis turns critical. It regulates tau phosphorylation connecting JNK1 with tauopathies observed in Alzheimer's disease. Researchers often investigate these associations to understand the mechanisms that underpin these conditions and develop targeted therapies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway (PubMed : 28943315). In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity (PubMed : 18307971). Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins (PubMed : 21856198). Loss of this interaction abrogates the acetylation required for replication initiation (PubMed : 21856198). Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1 (PubMed : 21364637). In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation (PubMed : 21095239). Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy (PubMed : 18570871). Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons (By similarity). In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone (By similarity). Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH (PubMed : 16581800, PubMed : 17296730, PubMed : 20027304). Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed : 22441692). Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity (PubMed : 10747973). Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteasomal degradation (By similarity). Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1 (PubMed : 22327296). In neurons, phosphorylates SYT4 which captures neuronal dense core vesicles at synapses (By similarity). Phosphorylates EIF4ENIF1/4-ET in response to oxidative stress, promoting P-body assembly (PubMed : 22966201). Phosphorylates SIRT6 in response to oxidative stress, stimulating its mono-ADP-ribosyltransferase activity (PubMed : 27568560). Phosphorylates NLRP3, promoting assembly of the NLRP3 inflammasome (PubMed : 28943315). Phosphorylates ALKBH5 in response to reactive oxygen species (ROS), promoting ALKBH5 sumoylation and inactivation (PubMed : 34048572).. JNK1 isoforms display different binding patterns : beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
See full target information MAPK8
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