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Rabbit Recombinant Monoclonal JNK1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, African green monkey, Zebrafish, Xenopus tropicalis, Chicken, Dog, Cow, Recombinant full length protein - Human samples. Cited in 224 publications.


Images

Immunocytochemistry/ Immunofluorescence - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461), expandable thumbnail
  • Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461), expandable thumbnail
  • Immunoprecipitation - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Tested
Expected
Rat
Expected
Tested
Expected
Expected
African green monkey
Expected
Tested
Expected
Expected
Chicken
Expected
Tested
Expected
Expected
Cow
Expected
Tested
Expected
Expected
Dog
Expected
Tested
Expected
Expected
Monkey
Predicted
Predicted
Predicted
Predicted
Recombinant full length protein - Human
Not recommended
Tested
Not recommended
Not recommended
Xenopus tropicalis
Expected
Tested
Expected
Expected
Zebrafish
Expected
Tested
Expected
Expected

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Expected
Expected

Species

Mouse, Rat, African green monkey, Zebrafish, Xenopus tropicalis, Chicken, Dog, Cow

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Monkey

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant full length protein - Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

African green monkey

Dilution info

1/1000

Notes

-

Species

Zebrafish

Dilution info

1/1000

Notes

-

Species

Xenopus tropicalis

Dilution info

1/1000

Notes

-

Species

Chicken

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Species

Dog

Dilution info

1/1000

Notes

-

Species

Cow

Dilution info

1/1000

Notes

-

Predicted
Predicted

Species

Monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/250

Notes

-

Species

Human

Dilution info

1/250

Notes

-

Expected
Expected

Species

Rat, African green monkey, Zebrafish, Xenopus tropicalis, Chicken, Dog, Cow

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Monkey

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/180

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse, Rat, African green monkey, Zebrafish, Xenopus tropicalis, Chicken, Dog, Cow

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Monkey

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity (PubMed:18307971). Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins (PubMed:21856198). Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1 (PubMed:21364637). In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation (PubMed:21095239). Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy (PubMed:18570871). Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH (PubMed:20027304, PubMed:17296730, PubMed:16581800). Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692). Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity (PubMed:10747973). Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteosomal degradation (By similarity). Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1 (PubMed:22327296). In neurons, phosphorylates SYT4 which captures neuronal dense core vesicles at synapses (By similarity). Phosphorylates EIF4ENIF1/4-ET in response to oxidative stress, promoting P-body assembly (PubMed:22966201).JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

Targets

Mitogen-activated protein kinase 9, MAPK10

Alternative names

Mitogen-activated protein kinase 8, MAP kinase 8, MAPK 8, JNK-46, Stress-activated protein kinase 1c, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, SAPK1c, SAPK1, SAPK1C, PRKM8, JNK1, MAPK8

Recommended products

Rabbit Recombinant Monoclonal JNK1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, African green monkey, Zebrafish, Xenopus tropicalis, Chicken, Dog, Cow, Recombinant full length protein - Human samples. Cited in 224 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR16797-211

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

JNK1 JNK2 and JNK3 collectively known as c-Jun N-terminal kinases are important members of the mitogen-activated protein kinase (MAPK) family. JNK1 JNK2 and JNK3 have molecular weights of approximately 46 to 55 kDa with JNK1 being around 46 kDa JNK2 about 54 kDa and JNK3 also around 54 kDa. They are expressed differently across tissues: JNK1 and JNK2 are widely present in many tissues while JNK3 is mostly in brain heart and testis. These kinases primarily phosphorylate serine and threonine residues acting on various transcription factors including c-Jun to regulate cellular processes.

Biological function summary

These kinases function as significant regulators of cellular responses to stress and cytokines. They do not act alone but often form part of larger signaling complexes that include other MAPKs and various scaffold proteins. Their roles are substantial in cell differentiation proliferation apoptosis and migration. Precise regulation by JNK molecules influences these critical cellular events which play a role in maintaining homeostasis and response to external stresses.

Pathways

JNK proteins are central to the MAPK signaling pathway and are tightly linked to the MAPK/ERK pathway. Activation of the JNK pathways leads to the regulation of various other proteins including those in the apoptosis pathway such as Bcl-2 and Bax. These interactions focus on cellular stress response regulation and contribute to processes like inflammation stress-induced apoptosis and some metabolic processes.

Associated diseases and disorders

JNKs have important roles in inflammatory diseases and neurodegenerative disorders such as Alzheimer's disease and Parkinson’s disease. Their activity links to the regulation of pro-inflammatory cytokines and neuronal apoptosis. JNK1 and JNK2 are particularly connected to inflammatory responses whereas JNK3 with its expression in the brain has associations with neuronal death in neurodegenerative diseases. Modulating JNK activity may offer therapeutic approaches for these conditions targeting pathways involving proteins like TNF-alpha and Aβ-amyloid.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling JNK1+JNK2+JNK3 with ab179461 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing both cytoplasmic and nuclear staining on HeLa cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:-
    -ve control 1: - ab179461 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: - Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

  • Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461), expandable thumbnail

    Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    JNKs originate from three genes that yield ten isoforms through alternative mRNA splicing, including JNK1α1, JNK1β1, JNK2α1, JNK2β1 and JNK3α1, which represent the p46 isoforms, and JNK1α2, JNK1β2, JNK2α2, JNK2β2 and JNK3β2, which represent the p54 isoforms.

    All lanes: Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461) at 1/20000 dilution

    Lane 1: K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysates at 20 µg

    Lane 2: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 20 µg

    Lane 3: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa

    Observed band size: 46 kDa, 54 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461)

    Formaldehyde-fixed, NP40 permeabilized Mouse Vascular smooth muscle cells stained for JNK1+JNK2+JNK3 (Green) using ab179461 at 1/200 dilution followed by a Donkey anti-rabbit Alex Fluor® 488 antibody at 1/500 dilution. The nuclear counterstain was DAPI (Blue).

  • Immunoprecipitation - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461), expandable thumbnail

    Immunoprecipitation - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461)

    JNK1+JNK2+JNK3 were immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extract with ab179461 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab179461 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: Jurkat whole cell extract. Lane 2: PBS instead of Jurkat whole cell extract.


    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    JNKs originate from three genes that yield ten isoforms through alternative mRNA splicing, including JNK1α1, JNK1β1, JNK2α1, JNK2β1 and JNK3α1, which represent the p46 isoforms, and JNK1α2, JNK1β2, JNK2α2, JNK2β2 and JNK3β2, which represent the p54 isoforms.

    All lanes: Immunoprecipitation - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461)

    Predicted band size: 48 kDa

  • Flow Cytometry (Intracellular) - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling JNK1+JNK2+JNK3 with purified ab179461 at 1/180 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461), expandable thumbnail

    Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461)

    Blocking/dilution buffer: 5% NFDM/TBST.

    JNKs originate from three genes that yield ten isoforms through alternative mRNA splicing, including JNK1α1, JNK1β1, JNK2α1, JNK2β1 and JNK3α1, which represent the p46 isoforms, and JNK1α2, JNK1β2, JNK2α2, JNK2β2 and JNK3β2, which represent the p54 isoforms.

    All lanes: Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461) at 1/5000 dilution

    Lane 1: Neuro-2a (Mouse neuroblastoma cells) whole cell lysates at 10 µg

    Lane 2: UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates at 10 µg

    Lane 3: MDCK (Canine kidney cell line) whole cell lysates at 10 µg

    Lane 4: MDBK (Bovine kidney cell line) whole cell lysates at 10 µg

    Lane 5: COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa

    Observed band size: 46 kDa, 54 kDa

  • Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461), expandable thumbnail

    Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461)

    Human JNK1, JNK2 and JNK3 full length recombinant proteins are from commercial sources. JNK1 and JNK2 have a proprietary tag, JNK3 has a His tag.

    Blocking/dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461) at 1/20000 dilution

    Lane 1: Human JNK3 full length recombinant protein containing a proprietary tag. at 0.01 µg

    Lane 2: Human JNK2 full length recombinant protein containing a proprietary tag. at 0.01 µg

    Lane 3: Human JNK1 full length recombinant protein containing a His tag. at 0.01 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa

    Observed band size: 48 kDa, 71 kDa

  • Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461), expandable thumbnail

    Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461)

    Blocking/dilution buffer: 5% NFDM/TBST.

    Zebrafish has only one JNK isoform, JNK1 with a MW of 44kDa. So there is only one band in Zebrafish.

    All lanes: Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461) at 1/1000 dilution

    Lane 1: Zebrafish lysate at 10 µg

    Lane 2: X. tropicalis lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa

    Observed band size: 46 kDa, 54 kDa

  • Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461), expandable thumbnail

    Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461)

    Blocking/dilution buffer: 5% NFDM/TBST.

    JNKs originate from three genes that yield ten isoforms through alternative mRNA splicing, including JNK1α1, JNK1β1, JNK2α1, JNK2β1 and JNK3α1, which represent the p46 isoforms, and JNK1α2, JNK1β2, JNK2α2, JNK2β2 and JNK3β2, which represent the p54 isoforms.

    All lanes: Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461) at 1/5000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: Rat brain lysate at 10 µg

    Lane 3: Rat heart lysate at 10 µg

    Lane 4: RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) lysate at 10 µg

    Lane 5: PC-12 (Rat adrenal gland pheochromocytoma) lysate at 10 µg

    Lane 6: NIH/3T3 (Mouse embyro fibroblast cells) lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa

    Observed band size: 46 kDa, 54 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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