AB288739

Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] - BSA and Azide free (Capture)

Recombinant
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Rabbit Recombinant Monoclonal JNK2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

JNK2, PRKM9, SAPK1A, MAPK9, Mitogen-activated protein kinase 9, MAP kinase 9, MAPK 9, JNK-55, Stress-activated protein kinase 1a, Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2, SAPK1a

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Sandwich ELISA - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] - BSA and Azide free (Capture) (AB288739)

sELISA

Supplier Data

Sandwich ELISA - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] - BSA and Azide free (Capture) (AB288739)

Sandwich ELISA of ab308540 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16797-211

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Rat, Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Anti-JNK 1/3 (Total) antibody [YCA-R16797-211 H1L1] - BSA and Azide free (Capture) (ab288739) is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify JNK1 + JNK2 + JNK3.

The recommended pair for sandwich ELISA is:

Capture: ab288739, JNK1 + JNK2 + JNK3 Capture Antibody (unconjugated)

Detector: ab308541, JNK1 + JNK2 + JNK3 Detector Antibody (unconjugated)

The reference range value is 0.39-50 ng/ml

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: 100% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The JNK protein family also known as c-Jun N-terminal kinases consists of JNK1 JNK2 and JNK3. These proteins are mitogen-activated protein kinases (MAPKs) that play a role in transmitting extracellular signals to the cell nucleus. JNK1 has a mass of approximately 54 kDa and is ubiquitously expressed in various tissues. JNK2 is similar in mass and expression while JNK3 is primarily found in the brain heart and testes. These proteins can phosphorylate transcription factors such as c-Jun impacting gene expression processes.

Biological function summary

The JNK proteins are involved in controlling cellular events like proliferation differentiation and apoptosis. They act as part of the MAPK signaling complex and involve scaffolding proteins that help organize these signaling pathways. In the cellular context JNK proteins are activated in response to stress signals like UV irradiation inflammatory cytokines and growth factors. This activation leads to diverse cellular outcomes depending on the specific JNK isoform and cell type.

Pathways

The JNK proteins are important in the MAPK signaling pathways and play a role in the AP-1 transcription factor regulation. They interact with proteins such as MEK4 and MEK7 which are upstream kinases that activate JNK through phosphorylation. The JNK signaling arm is distinct from the ERK and p38 MAPK pathways each having specific roles in cellular response modulation.

The JNK proteins are implicated in conditions like neurodegenerative diseases and cancer. In Alzheimer's disease for example JNK3 contributes to neuronal cell death while JNK inhibitors like SP600125 have been explored for therapeutic potential. In cancer JNK proteins can influence tumor progression through the regulation of apoptosis and their activity may intersect with other kinases like p53 further complicating disease pathways. JNK's role in diseases makes it a critical target for developing inhibitors reflected in studies around compounds like JNK inhibitor 8 and related molecules.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death (PubMed : 10376527, PubMed : 15805466, PubMed : 17525747, PubMed : 19675674, PubMed : 20595622, PubMed : 21364637, PubMed : 22441692, PubMed : 34048572). Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2 (PubMed : 10376527, PubMed : 15805466, PubMed : 17525747, PubMed : 19675674, PubMed : 20595622, PubMed : 21364637, PubMed : 22441692, PubMed : 34048572). In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity (PubMed : 10376527). In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3 (PubMed : 15805466). Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1 (PubMed : 17525747, PubMed : 21364637). In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells (PubMed : 19290929). Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels (PubMed : 19290929). Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption (PubMed : 20595622). When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway (PubMed : 19675674). Participates also in neurite growth in spiral ganglion neurons (By similarity). Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed : 22441692). Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteasomal degradation (By similarity). Phosphorylates ALKBH5 in response to reactive oxygen species (ROS), promoting ALKBH5 sumoylation and inactivation (PubMed : 34048572).. MAPK9 isoforms display different binding patterns : alpha-1 and alpha-2 preferentially bind to JUN, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it.

See full target information MAPK9

Additional targets

MAPK8,MAPK10

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] - BSA and Azide free (Capture)

Sandwich ELISA - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] - BSA and Azide free (Capture) (AB288739)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise