AB124956

Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693]

Name: Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (ab124956) | Abcam
Brand: Abcam Limited
SKU: AB124956
Price: 395 USD
Availability: InStock
Rating: 4 (8 reviews)

RabMAb
Recombinant
What is this?

(8 Reviews)

(320 Publications)

Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (ab124956) is a rabbit monoclonal antibody detecting JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) in Western Blot, Flow Cytometry (Intra), IP, IHC-P, ICC/IF, Dot Blot. Suitable for Human, Mouse.

- Biophysical QC for unrivalled batch-batch consistency
- Over 240 publications

View Alternative Names

JNK1, PRKM8, SAPK1, SAPK1C, MAPK8, Mitogen-activated protein kinase 8, MAP kinase 8, MAPK 8, JNK-46, Stress-activated protein kinase 1c, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, SAPK1c, JNK3, JNK3A, PRKM10, SAPK1B, MAPK10, Mitogen-activated protein kinase 10, MAP kinase 10, MAPK 10, MAP kinase p49 3F12, Stress-activated protein kinase 1b, Stress-activated protein kinase JNK3, c-Jun N-terminal kinase 3, SAPK1b, JNK2, PRKM9, SAPK1A, MAPK9, Mitogen-activated protein kinase 9, MAP kinase 9, MAPK 9, JNK-55, Stress-activated protein kinase 1a, Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2, SAPK1a

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (AB124956)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (AB124956)

ab124956, at 1/100 dilution staining JNK1+JNK2+JNK3 in paraffin-embedded Human brain tissue, by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (AB124956)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (AB124956)

Overlay histogram showing HeLa cells stained with ab124956 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124956, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was goat anti-rabbit Alexa Fluorr^® 488 IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Lab

Immunoprecipitation - Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (AB124956)

Purified ab124956 at 1/70 dilution (2μg) immunoprecipitating JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) in HeLa treated with 25ug/mL anisomycin for 30min whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 25ug/mL anisomycin for 30min whole cell lysate 10μg
Lane 2 (+) : ab124956 + HeLa treated with 25ug/mL anisomycin for 30min whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab124956 in HeLa treated with 25ug/mL anisomycin for 30min whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/5000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 46, 54 kDa

All lanes:

Immunoprecipitation - Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (ab124956)

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Immunocytochemistry/ Immunofluorescence - Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (AB124956)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (AB124956)

Immunocytochemistry/Immunofluorescence analysis of untreated, Anisomycin treated and Anisomycin + LP treated NIH/3T3 cells labelling JNK1 + JNK2 + JNK3 (phospho T183 + T183 + T221) with ab124956 at a dilution of 1/100 (left) and JNK1 + JNK2 + JNK3 with ab179461 at a dilution of 1/250 (right).

Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

The image shows increased nuclear staining after Anisomycin (250ng/ml, 30min) treatment on NIH3T3 cells. The LP treatment decreased the increased nuclear staining caused by Anisomycin.

ab179461 was used as a Pan control for ab124956. The results showed cytoplasmic staining on untreated, Anisomycin and Anisomycin + LP treated NIH3T3 cells.

Lab

Western blot - Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (AB124956)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (ab124956) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) Whole cell lysates at 15 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysates at 15 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysates. Then the membrane was incubated with alkaline phosphatase at 15 µg

Lane 4:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysates. Then the membrane was incubated with lambda phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 46 kDa,54 kDa

false

Exposure time: 30s

Unknown

Western blot - Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (AB124956)

Secondary antibody - goat anti-rabbit HRP (ab6721)

All lanes:

Western blot - Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (ab124956) at 1/1000 dilution

Lane 1:

NIH 3T3 cell lysate, untreated at 10 µg

Lane 2:

NIH 3T3 cell lysate, treated with Anisomycin at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

false

OI-RD Scanning - Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (AB124956)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (AB124956)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Lab

Dot Blot - Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (AB124956)

Dot blot analysis of JNK1/2/3 (pT183 + pT183 + pT221) peptide (Lane 1) and JNK1/2/3 non-phospho peptide (Lane 2) labelling JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) with ab124956 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Carrier free

Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] - BSA and Azide free
578 PE

PE Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693]
660 APC

APC Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5693

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, Dot, IHC-P, IP, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody will detect will detect JNK1 (pT183), JNK2 (pT183) and JNK3 (pT221).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "(Heat to 98°C, allow to cool for 10-20 minutes) Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Synthetic peptide - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (ab124956) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), Dot Blot in Human, Mouse samples.

Trusted by the scientific community
Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) [EPR5693] (ab124956) was first used in a scientific publication in 2012 and has been cited over 240 times in peer-reviewed journals.

Reviewed by scientists
Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) [EPR5693] (ab124956) has over 5 independent reviews from customers.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR5693] also available for your convenience: ab124956, Alexa Fluor® 488 - ab201862, Alexa Fluor® 647 - ab201864, PE - ab208843, Carrier free - ab219584, APC - ab310817, Alexa Fluor® 594 - ab311653, Alexa Fluor® 568 - ab312926, Alexa Fluor® 555 - ab313139, Alexa Fluor® 750 - ab320994

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

JNK1 JNK2 and JNK3 are c-Jun N-terminal kinases also known as stress-activated protein kinases (SAPKs). These kinases play significant roles in cellular stress responses. Commonly referred to by their shorter names JNK1 JNK2 and JNK3 they possess molecular weights of approximately 46 kDa to 55 kDa depending on their isoforms. These kinases are widely expressed across various tissues with JNK1 and JNK2 seen in most tissues whereas JNK3 shows more expression in neural tissues. Activation of JNKs occurs through phosphorylation at threonine 183 (T183) for JNK1 and JNK2 and threonine 221 (T221) for JNK3.

Biological function summary

C-Jun N-terminal kinases play important roles in mediating responses to stress stimuli including cytokines and ultraviolet irradiation. They are known to be part of the MAPK signaling complex and have direct involvement in the regulation of genes connected to apoptosis and cellular proliferation. JNK1 and JNK2 in particular have broad cellular roles and influence the activity of transcription factors such as c-Jun impacting gene expression significantly. JNK3 meanwhile contributes more to neuronal apoptosis given its expression pattern.

Pathways

JNK1 JNK2 and JNK3 actively engage in the MAPK signaling pathway connecting with several upstream and downstream proteins like MKK4 and MKK7 which serve as upstream kinases and c-Jun which acts downstream. The MAPK pathway is critical for translating extracellular signals into a wide range of cellular processes. The involvement of JNKs in this pathway highlights their contribution to balancing cell survival and death signals placing them alongside related proteins like ERK and p38MAPK within the signaling hierarchy.

The activation and regulation of JNK1 JNK2 and JNK3 have essential implications in neurodegenerative diseases and cancer. JNK3 in particular is associated with neurodegenerative conditions such as Alzheimer's disease due to its role in neuronal stress-induced apoptosis. Similarly aberrant JNK1 and JNK2 activation connects to various cancers through their influence on genes governing cell cycle and apoptosis often in tandem with oncogenes or tumor suppressors like p53 and Bcl-2. These associations highlight the therapeutic potential of targeting these kinases in disease treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway (PubMed : 28943315). In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity (PubMed : 18307971). Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins (PubMed : 21856198). Loss of this interaction abrogates the acetylation required for replication initiation (PubMed : 21856198). Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1 (PubMed : 21364637). In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation (PubMed : 21095239). Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy (PubMed : 18570871). Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons (By similarity). In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone (By similarity). Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH (PubMed : 16581800, PubMed : 17296730, PubMed : 20027304). Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed : 22441692). Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity (PubMed : 10747973). Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteasomal degradation (By similarity). Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1 (PubMed : 22327296). In neurons, phosphorylates SYT4 which captures neuronal dense core vesicles at synapses (By similarity). Phosphorylates EIF4ENIF1/4-ET in response to oxidative stress, promoting P-body assembly (PubMed : 22966201). Phosphorylates SIRT6 in response to oxidative stress, stimulating its mono-ADP-ribosyltransferase activity (PubMed : 27568560). Phosphorylates NLRP3, promoting assembly of the NLRP3 inflammasome (PubMed : 28943315). Phosphorylates ALKBH5 in response to reactive oxygen species (ROS), promoting ALKBH5 sumoylation and inactivation (PubMed : 34048572).. JNK1 isoforms display different binding patterns : beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

See full target information MAPK8 phospho T183

Additional targets

MAPK9 phospho T183,MAPK10 phospho T221

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Publications (320)

Recent publications for all applications. Explore the full list and refine your search

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology : PubMed40947964

2025

Eupatilin alleviates right ventricular fibrosis in rats with pulmonary hypertension induced by monocrotaline.

Applications

Unspecified application

Species

Unspecified reactive species

Tonggang Zhu,Xue Xiao,Xue Li,Zhenkun Liu

Mediators of inflammation 2025:4932970 PubMed40918406

2025

Electroacupuncture Attenuates Hepatic Ischemia-Reperfusion Injury by Modulating the Esr1/TAK1-JNK/p38 Signaling Pathway in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofang Fan,Wei Guo,Xiaodan Yang,Hao Zhang,Bruno Fink,Lingyu Hu,Xiaoguang Wang

Cellular and molecular life sciences : CMLS 82:269 PubMed40610735

2025

Cell division cycle protein 42-driven activation of the MKK3/6-p38 signaling pathway participates in cardiac remodeling in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Wen,Lin Xie,Quan-Wen Liu,Guan-Hui Yu,Xu-Hui Qiao,Yu-Chun Huang,Lu Wang,Xin Li,Li-Dan Wen,Xiao-Lei Wang,Jing He,Xin-Yu Xiao,Xiao-Xiao Zhao,Ling-Fang Wang,Hong-Bo Xin,Ke-Yu Deng

Molecular therapy. Nucleic acids 36:102555 PubMed40487353

2025

CircITSN1/EIF4A3/Itsn1 axis mediates postoperative cognitive dysfunction in aged mice: A novel mechanism and therapeutic target.

Applications

Unspecified application

Species

Unspecified reactive species

Changteng Zhang,Xiaoyu Zhu,Rui Gao,Hai Chen,Caiyi Yan,Wangyang Liu,Lina Yang,Xianzheng Zeng,Haoran Yang,Jin Liu,Qi Li,Daqing Ma,Tao Zhu,Chan Chen

American journal of cancer research 15:1759-1776 PubMed40371147

2025

Prognostic value, biological role, and mechanisms of LCN2 in childhood acute lymphoblastic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Tang,Yuan-Yuan Li,Lin-Jun Tan,Ju Gao,Zhi-Gui Ma,Xia Guo,Ling Gu,Han-Min Liu

RSC medicinal chemistry : PubMed40236619

2025

Rutaecarpine derivatives synthesized skeletal reorganization alleviate inflammation-associated oxidative damage by inhibiting the MAPK/NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Nan-Ying Chen,Cai-Neng Zhang,Xiu-Yun Guo,Liu-Song Lan,Yi-Fan Geng,Jin-Hui Peng,Cheng-Xue Pan,Yan Huang,Gui-Fa Su

BMC medicine 23:209 PubMed40189495

2025

Double-negative T cells in combination with ursodeoxycholic acid ameliorates immune-mediated cholangitis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Chunpan Zhang,Guangyong Sun,Hua Jin,Yunxiong Wei,Shimeng Zheng,Xiyu Wang,Xinyan Zhao,Dong Zhang,Jidong Jia

Cell reports. Medicine 6:102000 PubMed40056904

2025

LTA4H improves the tumor microenvironment and prevents HCC progression via targeting the HNRNPA1/LTBP1/TGF-β axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Yang,Xinyao Qiu,Yingcheng Yang,Jing Wu,Shan Wang,Bo Zheng,Jianmin Wu,Tao Zhou,Yangqianwen Zhang,Mixue Bai,Shuowu Liu,Zihan Zhao,Yani Zhang,Yixian Wang,Jinxia Bao,Mengye Wu,Dongdong Xue,Meiyu Bao,Ji Hu,Siyun Shen,Hongyang Wang,Lei Chen

CNS neuroscience & therapeutics 30:e70190 PubMed39722194

2024

The JNK Signaling Pathway Regulates Seizures Through ENT1 in Pilocarpine-Induced Epilepsy Rat Model.

Applications

Unspecified application

Species

Unspecified reactive species

Shun Liu,Zhong Luo,Fangjing Li,Lijia Zhang,Mingxiang Xie,Juan Yang,Zucai Xu

Molecular and cellular biochemistry 480:3147-3160 PubMed39690293

2024

Interleukin-22 promotes endometrial carcinoma cell proliferation and cycle progression via ERK1/2 and p38 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Shiqi Liu,Ruqian Zhao,Yuqin Zang,Pengzhu Huang,Qiaoling Zhang,Xiangqin Fan,Junyi Bai,Xingyu Zheng,Shuangshuang Zhao,Dan Kuai,Chao Gao,Yingmei Wang,Fengxia Xue

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (AB124956)

Flow Cytometry (Intracellular) - Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (AB124956)

Immunoprecipitation - Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (AB124956)

Immunocytochemistry/ Immunofluorescence - Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (AB124956)

Western blot - Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (AB124956)

Western blot - Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (AB124956)

OI-RD Scanning - Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (AB124956)

Dot Blot - Anti-JNK1 + JNK2 + JNK3 (phospho T183+T183+T221) antibody [EPR5693] (AB124956)

Related conjugates and formulations (9)

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